Induction of cartilage growth in a rabbit ear model: a pilot study

Ad‐El, Dean; Selah, Jonah; Goshen, Gal; Dano, Izhac

doi:10.1007/s00238-006-0048-z

Cited by 5 publications

(8 citation statements)

References 15 publications

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“…We selected the rabbit ear as a model to test the effectiveness of the microbeads in regenerating cartilage based on published reports using this method (Ad‐El, Selah, Goshen, & Dano, ; Mirzadeh et al, ) We were able to define criteria for a critical size defect in the rabbit ear cartilage. Based on our findings, the 3‐mm defect was chosen due to its size for microbead implantation and ease of manipulation during surgery.…”

Section: Discussionmentioning

confidence: 99%

Microencapsulated rabbit adipose stem cells initiate tissue regeneration in a rabbit ear defect model

Leslie

Cohen

Hyzy

et al. 2018

J Tissue Eng Regen Med

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Cell-based tissue engineering can promote cartilage tissue regeneration, but cell retention in the implant site post-delivery is problematic. Alginate microbeads containing adipose stem cells (ASCs) pretreated with chondrogenic media have been used successfully to regenerate hyaline cartilage in critical size defects in rat xiphoid suggesting that they may be used to treat defects in elastic cartilages such as the ear. To test this, we used microbeads made with low viscosity, high mannuronate medical grade alginate using a high electrostatic potential, and a calcium cross linking solution containing glucose. Microbeads containing rabbit ASCs (rbASCs) were implanted bilaterally in 3 mm critical size midcartilage ear defects of six skeletally mature male New Zealand White rabbits (empty defect; microbeads without cells; microbeads with cells; degradable microbeads with cells; and autograft). Twelve weeks post-implantation, regeneration was assessed by microCT and histology. Microencapsulated rbASCs cultured in chondrogenic media expressed mRNAs for aggrecan, Type II collagen, and Type X collagen. Histologically, empty defects contained fibrous tissue; microbeads without cells were still present in defects and were surrounded by fibrous tissue; nondegradable beads with rbASCs initiated cartilage regeneration; degradable microbeads with cells produced immature bone-like tissue, also demonstrated by microCT; and autografts appeared as normal auricular cartilage but were not fully integrated with the tissue surrounding the defect. Elastin, the hallmark of auricular cartilage, was not evident in the neocartilage. This delivery system offers the potential for regeneration of auricular cartilage, but vascularity of the treatment site and use of factors that induce elastin must be considered.

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Section: Discussionmentioning

confidence: 99%

Microencapsulated rabbit adipose stem cells initiate tissue regeneration in a rabbit ear defect model

Leslie

Cohen

Hyzy

et al. 2018

J Tissue Eng Regen Med

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“…Each ear received two 2-mmdiameter wounds on the medial surface with removal of medial skin and cartilage. One wound was created with the residual of the lateral skin together with one side of perichondrium, and the other wound was the lateral skin without perichondrium (Ulutaş et al, 2005;Ad-El et al, 2006). A schematic drawing of the wound model is shown in Figure 1 to illustrate the two regions of interest (ROI1 and ROI2).…”

Section: Animal Models and Tissue Specimensmentioning

confidence: 99%

“…and two-photon excited fluorescence (TPEF). Elastic cartilage contains chondrocytes and extracellular matrix including collagen and elastin fibres (Ad-El et al, 2006). In newly generated cartilage, collagen and elastic fibres are the principal determinants of the macromolecular structure and are associated with the mechanical properties that are central to its biomechanical function.…”

Section: Introductionmentioning

confidence: 99%

Differentiating the extent of cartilage repair in rabbit ears using nonlinear optical microscopy

Zhu

Liao

et al. 2015

Journal of Microscopy

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Nonlinear optical microscopy (NLOM) was used as a noninvasive and label-free tool to detect and quantify the extent of the cartilage recovery. Two cartilage injury models were established in the outer ears of rabbits that created a different extent of cartilage recovery based on the presence or absence of the perichondrium. High-resolution NLOM images were used to measure cartilage repair, specifically through spectral analysis and image texture. In contrast to a wound lacking a perichondrium, wounds with intact perichondria demonstrated significantly larger TPEF signals from cells and matrix, coarser texture indicating the more deposition of type I collagen. Spectral analysis of cells and matrix can reveal the matrix properties and cell growth. In addition, texture analysis of NLOM images showed significant differences in the distribution of cells and matrix of repaired tissues with or without perichondrium. Specifically, the decay length of autocorrelation coefficient based on TPEF images is 11.2 ± 1.1 in Wound 2 (with perichondrium) and 7.5 ± 2.0 in Wound 1 (without perichondrium), indicating coarser image texture and faster growth of cells in repaired tissues with perichondrium (p < 0.05). Moreover, the decay length of autocorrelation coefficient based on collagen SHG images also showed significant difference between Wound 2 and 1 (16.2 ± 1.2 vs. 12.2 ± 2.1, p < 0.05), indicating coarser image texture and faster deposition of collagen in repaired tissues with perichondrium (Wound 2). These findings suggest that NLOM is an ideal tool for studying cartilage repair, with potential applications in clinical medicine. NLOM can capture macromolecular details and distinguish between different extents of cartilage repair without the need for labelling agents.

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“…Previous studies have claimed that perichondrium of the adult ear is a useful source of cells that can self‐renew and can contribute to tissue reconstruction. They demonstrated that auricular cartilage with perichondrium can self‐regenerate, but differing from the original cartilage in its cell morphology, and the amount and distribution of intercellular matrix (Ad‐El et al ., ; Kobayashi et al ., ; Mo et al ., ). During the regeneration, cells in the perichondrium promote the deposition of extracellular matrix (ECM) including collagen and elastic fibers, and the newly synthesized ECM proteins can inversely support cells to live, proliferate, and function within it (Mansfi et al ., ; Kobayashi et al ., ).…”

Section: Introductionmentioning

confidence: 98%

Stereoscopic visualization and quantification of auricular cartilage regeneration in rabbits using multiphoton microscopy

Chen

Zhu

et al. 2014

Scanning

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Multiphoton microscopy (MPM) was applied for imaging and quantifying the elastic cartilage regeneration tissue in a rabbit ear model without using labeling agents. Morphology of cells and collagen matrix were analysis, showing significant difference between regenerated and intact cartilage in cellular size and collagen distribution. The results demonstrate that high resolution images provide by MPM are consistent with the histological results, and show additional biological behavior which is not visible in standard histology. Advantages in instrumentation may lead to the application of MPM for intravital detection and treatment.

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Induction of cartilage growth in a rabbit ear model: a pilot study

Cited by 5 publications

References 15 publications

Microencapsulated rabbit adipose stem cells initiate tissue regeneration in a rabbit ear defect model

Microencapsulated rabbit adipose stem cells initiate tissue regeneration in a rabbit ear defect model

Differentiating the extent of cartilage repair in rabbit ears using nonlinear optical microscopy

Stereoscopic visualization and quantification of auricular cartilage regeneration in rabbits using multiphoton microscopy

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