Induction of CCK and GLP-1 release in enteroendocrine cells by egg white peptides generated during gastrointestinal digestion

Santos-Hernández, Marta; Amigo, Lourdes; Recio, Isidra

doi:10.1016/j.foodchem.2020.127188

Cited by 27 publications

(37 citation statements)

References 28 publications

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“…In accordance, in a recent work, two peptides able to stimulate CCK secretion in STC-1 cells, VLLPDEVSGL and VLLPD, were identified from an egg white SGID intestinal digest. They both did not contain aromatic amino acid residues but are characterized by a high rate of aliphatic ones [48].…”

Section: Discussionmentioning

confidence: 99%

“…Nevertheless, different signaling pathways, involving G protein-coupled receptors (GPCRs) like GPR93, GPRC6A and the calcium-sensing receptor (CaSR) but also the cotransporter PepT-1, have been evidenced in the food protein-derived peptide intestinal sensing leading to CCK and GLP-1 secretion [39][40][41]. CCK releasing food-derived peptides were identified from soybean β-conglycinin, bovine hemoglobin, lactoglobulin, bovine whey, casein, and egg white protein [42][43][44][45][46][47][48]. To our knowledge, it is the first time from fish source.…”

Section: Discussionmentioning

confidence: 99%

“…However, the K residue position in the sequence does not seem crucial for the bioactivity as ASDKPYIL is also active [51]. The RVASMASEKM peptide, recently identified from egg white protein digest as GLP-1 secretagogue, also possesses a K residue but in C-terminal position [48]. Nevertheless, results obtained here showed that ELLK and EAPLNPK did not lead to GLP-1 secretion, and other works identified peptides able to stimulate GLP-1 secretion without having K or T residues in their sequences, such as GGGG, AAAA, GWGG [52], GPVRGPFPIIV [53], LGG and GF [54] and, PFL [48].…”

Section: Discussionmentioning

confidence: 99%

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New Bioactive Peptides Identified from a Tilapia Byproduct Hydrolysate Exerting Effects on DPP-IV Activity and Intestinal Hormones Regulation after Canine Gastrointestinal Simulated Digestion

et al. 2020

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Like their owners, dogs and cats are more and more affected by overweight and obesity-related problems and interest in functional pet foods is growing sharply. Through numerous studies, fish protein hydrolysates have proved their worth to prevent and manage obesity-related comorbidities like diabetes. In this work, a human in vitro static simulated gastrointestinal digestion model was adapted to the dog which allowed us to demonstrate the promising effects of a tilapia byproduct hydrolysate on the regulation of food intake and glucose metabolism. Promising effects on intestinal hormones secretion and dipeptidyl peptidase IV (DPP-IV) inhibitory activity were evidenced. We identify new bioactive peptides able to stimulate cholecystokinin (CCK) and glucagon-like peptide 1 (GLP-1) secretions, and to inhibit the DPP-IV activity after a transport study through a Caco-2 cell monolayer.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

New Bioactive Peptides Identified from a Tilapia Byproduct Hydrolysate Exerting Effects on DPP-IV Activity and Intestinal Hormones Regulation after Canine Gastrointestinal Simulated Digestion

et al. 2020

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“…Selected tetrapeptides were identified to induce GLP-1 release from the human enteroendocrine cell line NCI-H716 [ 59 ]. A recent study demonstrated that a hydrophobic, positively charged peptide from egg white protein possesses potent GLP-1 releasing activity [ 60 ].…”

Section: Dietary Proteins/peptides/amino Acids and Glp-1mentioning

confidence: 99%

“…On the other hand, some small active peptides might be liberated after sufficient digestion in the small intestine, but they are immediately absorbed into epithelial cells before acting on GLP-1-producing cells. Despite these limitations, INFOGEST [ 62 ], a static in vitro simulation of gastrointestinal food digestion, helps identify and estimate peptide fragments after luminal digestion [ 60 ].…”

Section: Dietary Proteins/peptides/amino Acids and Glp-1mentioning

confidence: 99%

Improvement of Glucose Tolerance by Food Factors Having Glucagon-Like Peptide-1 Releasing Activity

Hira

Trakooncharoenvit

Taguchi

et al. 2021

IJMS

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Glucagon-like peptide-1 (GLP-1) is a gastrointestinal hormone released from enteroendocrine L cells in response to meal ingestion. GLP-1 receptor agonists and GLP-1 enhancers have been clinically employed to treat diabetes owing to their glucose-dependent insulin-releasing activity. The release of GLP-1 is primarily stimulated by macronutrients such as glucose and fatty acids, which are nutritionally indispensable; however, excessive intake of sugar and fat is responsible for the development of obesity and diabetes. Therefore, GLP-1 releasing food factors, such as dietary peptides and non-nutrients, are deemed desirable for improving glucose tolerance. Human and animal studies have revealed that dietary proteins/peptides have a potent effect on stimulating GLP-1 secretion. Studies in enteroendocrine cell models have shown that dietary peptides, amino acids, and phytochemicals, such as quercetin, can directly stimulate GLP-1 secretion. In our animal experiments, these food factors improved glucose metabolism and increased GLP-1 secretion. Furthermore, some dietary peptides not only stimulated GLP-1 secretion but also reduced plasma peptidase activity, which is responsible for GLP-1 inactivation. Herein, we review the relationship between GLP-1 and food factors, especially dietary peptides and flavonoids. Accordingly, utilization of food factors with GLP-1-releasing/enhancing activity is a promising strategy for preventing and treating obesity and diabetes.

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In vitro gastrointestinal digestion of highland barley protein: identification and characterization of novel bioactive peptides involved in gut cholecystokinin secretion

et al. 2023

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BACKGROUNDThe satiety hormone cholecystokinin (CCK) plays an important role in food intake inhibition. Its secretion is regulated by dietary components. The search for bioactive compounds that induce CCK secretion is currently an active area of research. The objective of this study was to evaluate the ability of highland barley protein digest (HBPD) to stimulate CCK secretion in vitro and in vivo and identify the responsible peptide sequences.RESULTSHBPD was prepared by in vitro gastrointestinal digestion model. Peptides of <1000 Da in HBPD accounted for 82%. HBPD was rich in essential amino acids Leu, Phe and Val, but lack in sulfur amino acids Met and Cys. HBPD treatment at a concentration of 5 mg mL−1 significantly stimulated CCK secretion from enteroendocrine STC‐1 cells (P < 0.05). Moreover, oral gavage with HBPD in mice significantly increased plasma CCK level. Chromatographic separation was performed to isolate peptide fractions involved in CCK secretion and peptide sequence was determined by ultra‐performance liquid chromatography–tandem mass spectrometry. Two novel CCK‐releasing peptides, PDLP and YRIVPL, were pointed out for their outstanding CCK secretagogue activity.CONCLUSIONThis study demonstrated for the first time that HBPD had an ability to stimulate CCK secretion in vitro and in vivo and determined the bioactive peptides exerting CCK secretagogue activity in HBPD. © 2023 Society of Chemical Industry.

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Induction of CCK and GLP-1 release in enteroendocrine cells by egg white peptides generated during gastrointestinal digestion

Cited by 27 publications

References 28 publications

New Bioactive Peptides Identified from a Tilapia Byproduct Hydrolysate Exerting Effects on DPP-IV Activity and Intestinal Hormones Regulation after Canine Gastrointestinal Simulated Digestion

New Bioactive Peptides Identified from a Tilapia Byproduct Hydrolysate Exerting Effects on DPP-IV Activity and Intestinal Hormones Regulation after Canine Gastrointestinal Simulated Digestion

Improvement of Glucose Tolerance by Food Factors Having Glucagon-Like Peptide-1 Releasing Activity

In vitro gastrointestinal digestion of highland barley protein: identification and characterization of novel bioactive peptides involved in gut cholecystokinin secretion

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