Induction of cementogenesis and periodontal ligament regeneration by recombinant human transforming growth factor‐β3 in Matrigel with rectus abdominis responding cells

Abstract

Matrigel matrix is an optimal delivery system for the osteogenic proteins of the transforming growth factor-beta superfamily, including the mammalian transforming growth factor-beta3 isoform. The addition of minced fragments of rectus abdominis muscle provides responding stem cells for further tissue induction and morphogenesis by the transforming growth factor-beta3 protein.

Tissue engineered bone using select growth factors: A comprehensive review of animal studies and clinical translation studies in man

“…TGF-β3 is a potent chondrogenic growth factor enhancing hyaline cartilage formation in vivo (Ripamonti et al, 2009a;Tang et al, 2009). Ripamonti et al (2009b; and Teare et al (2008) investigated the direct delivery of TGF-β3 (5 to 125 µg) within adult Chacma baboons and demonstrated a 1.75-to 3-fold increase in bone formation (Supplementary Table 7). Direct TGF-β3 delivery (3 ng to 2.75 µg) within small animal models, including mouse (Kovacevic et al, 2011) and rat (Opperman et al, 2002), only showed a 1.05 fold increase in bone volume.…”

Redefining the induction of periodontal tissue regeneration in primates by the osteogenic proteins of the transforming growth factor‐β supergene family

“…A–C) is fragmented, morcellated (Fig. D) and transplanted in class II and III furcation defects of P. ursinus . Morphological analyses on undecalcified sections cut at 6 μm showed the induction of periodontal tissue regeneration with prominent angiogenesis with a capillary patterning at the cementum interface setting the repetitive insertion and alignment of newly formed Sharpey's fibers (Fig.…”

“…) vs. the transplantation of fragments of heterotopic ossicles induced by different doses of the hTGF‐β 3 protein (Fig. ) resulted in highly comparable periodontal tissue regeneration in class II furcation defects of P. ursinus . The rapid architectural sculpture of mineralized corticalized constructs in the rectus abdominis muscle of P. ursinus by the hTGF‐β 3 isoform is a novel source of developing auto‐induced bone for autogenous transplantation in preclinical and clinical contexts (Fig.…”

“…The induction of bone formation is not limited to extraskeletal rectus abdominis heterotopic sites but encompasses significant amounts of bone formation in full‐thickness segmental mandibular defects of P. ursinus and alveolar bone regeneration with embedded Sharpey's fibers uniting the alveolar bone to the newly formed cementum (Figs and ) . Two novel and provocative treatments recently performed in P. ursinus using the recombinant human TGF‐β 3 (hTGF‐β 3 ; kindly provided by Novartis AG, Zurich, Switzerland) induced substantial periodontal tissue regeneration in class II and III furcation defects of the first and second mandibular molars (Figs and ) .…”

Periodontal Ligament Stem Cells

“…Interestingly, Chen et al revealed that BMP-2-loaded pellets incorporated into glycidyl methacrylated dextran/gelatin scaffolds stimulated not only bone and cementum formation, but also PDL-like connective tissue regeneration . Implantation of TGF-β3 in lyophilized Matrigel ® scaffolds in baboon Class III furcation defects with minced fragments of rectus abdominis muscle reportedly induced responding stem cells to stimulate the formation of large amounts of alveolar bone with associated PDL fibers and newly formed cementum (Ripamonti et al, 2009). …”