Induction of chromosomal and DNA damage and histological alterations by graphene oxide nanoparticles in Swiss mice

Mohamed, Hanan R. H.; Welson, Mary; Yaseen, Ahmed E.; El-Ghor, Akmal A.

doi:10.1080/01480545.2019.1643876

Cited by 26 publications

(20 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This could lead to DNA damage and probably to the accumulation of DNA mutations 74 . Preliminary evidence already exists for DNA damage generated by different GRMs 75 – 77 and it is worth highlighting the relevance of lateral size, oxidation, dose, etc. in DNA damage.…”

Section: Discussionmentioning

confidence: 99%

Sublethal exposure of small few-layer graphene promotes metabolic alterations in human skin cells

Frontiñán-Rubio

Gómez

González

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Small few-layer graphene (sFLG), a novel small-sized graphene-related material (GRM), can be considered as an intermediate degradation product of graphene. GRMs have a promising present and future in the field of biomedicine. However, safety issues must be carefully addressed to facilitate their implementation. In the work described here, the effect of sub-lethal doses of sFLG on the biology of human HaCaT keratinocytes was examined. A one-week treatment of HaCaTs with sub-lethal doses of sFLG resulted in metabolome remodeling, dampening of the mitochondrial function and a shift in the redox state to pro-oxidant conditions. sFLG raises reactive oxygen species and calcium from 24 h to one week after the treatment and this involves the activation of NADPH oxidase 1. Likewise, sFLG seems to induce a shift from oxidative phosphorylation to glycolysis and promotes the use of glutamine as an alternative source of energy. When sub-toxic sFLG exposure was sustained for 30 days, an increase in cell proliferation and mitochondrial damage were observed. Further research is required to unveil the safety of GRMs and degradation-derived products before their use in the workplace and in practical applications.

show abstract

Section: Discussionmentioning

confidence: 99%

Sublethal exposure of small few-layer graphene promotes metabolic alterations in human skin cells

Frontiñán-Rubio

Gómez

González

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…Depending on the dose of GO nanoparticle, a significant increase in micronuclei and DNA damage was reported in the study group mice compared to the control group mice. In addition, it has been reported that apoptosis, necrosis, inflammations, and cell degeneration in the liver and brain tissue sections, including histological lesions [25]. Overall these studies indicate that high concentration of GBMs, extended incubation times, dose-depended and ultra-sharp edges cause genotoxicity in vivo.…”

Section: Introductionmentioning

confidence: 67%

A closer look at the genotoxicity of graphene based materials

et al. 2019

View full text Add to dashboard Cite

Graphene-based materials (GBMs) have attracted many scientists because of their optical, thermal, mechanical and electronic properties. Their good dispersibility in different type of solvents including water, the possibility to formulate them according to desired function, and the wide surface area, which can allow various chemical modifications, expanded the use of these materials in biological systems. For these reasons, GBMs have been extensively studied in vitro and in vivo in the biomedical field. However, the toxicity and genotoxicity of GBMs must be thoroughly investigated before they can be translated into clinical settings. The main mechanism of graphene toxicity is thought to be caused by reactive oxygen species produced in cells, which in turn interact with various biomolecules including DNA. In this review we aimed to discuss different genotoxicity studies performed with GBMs with specific focus on the different cell types and conditions. By comparing and discussing such reports, scientists will be able to engineer non toxic GBMs for future preclinical and/or clinical studies. In order to allow a safer and faster transition to clinic, future studies should involve state-ofthe-art technologies such as systems biology approaches or three-dimensional microfluidic systems, which can better predict the normal physiological scenario.

show abstract

“…Thirteen studies, published in seven papers, assessed the in vivo genotoxicity of graphene ( Table 7 ). Among them, ten studied the effect of GO [ 60 , 96 , 97 , 98 , 99 ], two of rGO [ 97 , 100 ] and one of exfoliated graphene [ 91 ]. Regarding graphene material characterization, it was not found in two of the publications; in the others, the amount of information was not the same among different studies but, in general, lateral size, z-potential, and hydrodynamic diameter are provided.…”

Section: Resultsmentioning

confidence: 99%

Genotoxicity of Graphene-Based Materials

et al. 2022

View full text Add to dashboard Cite

Graphene-based materials (GBMs) are a broad family of novel carbon-based nanomaterials with many nanotechnology applications. The increasing market of GBMs raises concerns on their possible impact on human health. Here, we review the existing literature on the genotoxic potential of GBMs over the last ten years. A total of 50 articles including in vitro, in vivo, in silico, and human biomonitoring studies were selected. Graphene oxides were the most analyzed materials, followed by reduced graphene oxides. Most of the evaluations were performed in vitro using the comet assay (detecting DNA damage). The micronucleus assay (detecting chromosome damage) was the most used validated assay, whereas only two publications reported results on mammalian gene mutations. The same material was rarely assessed with more than one assay. Despite inhalation being the main exposure route in occupational settings, only one in vivo study used intratracheal instillation, and another one reported human biomonitoring data. Based on the studies, some GBMs have the potential to induce genetic damage, although the type of damage depends on the material. The broad variability of GBMs, cellular systems and methods used in the studies precludes the identification of physico-chemical properties that could drive the genotoxicity response to GBMs.

show abstract

Induction of chromosomal and DNA damage and histological alterations by graphene oxide nanoparticles in Swiss mice

Cited by 26 publications

References 38 publications

Sublethal exposure of small few-layer graphene promotes metabolic alterations in human skin cells

Sublethal exposure of small few-layer graphene promotes metabolic alterations in human skin cells

A closer look at the genotoxicity of graphene based materials

Genotoxicity of Graphene-Based Materials

Contact Info

Product

Resources

About