Induction of chromosomal and morphological amelioration in lentil (Lens culinaris Medik.) mutagenized population developed through chemical mutagenesis

Sharma, Nidhi; Shahwar, Durre; Choudhary, Sana

doi:10.1007/s42535-021-00319-6

Cited by 5 publications

(6 citation statements)

References 16 publications

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“…Moreover, azide anions are potent proton pump inhibitors ( Kleinhofs et al., 1978 ) altering the mitochondrial membrane potential ( Zhang et al., 2000 ), resulting in a shortage of ATP and slowing down the germination. This result is consistent with earlier studies on several crops, including Cajanus cajan ( Saroj et al., 2016 ), Coriandrum sativum ( Kolhe et al., 2020 ), Trigonella foenum-graecum ( Hassan et al., 2018 ; Naaz et al., 2020 ), and Lens culinaris ( Sharma et al., 2022 ). The survival at maturity was reduced with an increasing concentration of mutagens due to cytogenetic aberrations and physiological instabilities ( Sato and Gaul, 1967 ) and disruption in the stability among inhibitors of growth regulators and promoters ( Meherchandani, 1975 ).…”

Section: Discussionsupporting

confidence: 92%

“…The diminution in quantitative traits at higher concentrations may arise due to physiological disturbance or DNA damage triggered in the plant cells by the mutagen (Thilagavathi and Mullainathan, 2011). In this study, these observations were supported by Amin et al (2015) and Sharma et al (2022) in lentil and Hasan et al (2020) in chili.…”

Section: Discussionsupporting

confidence: 89%

“…In this study, these observations were supported by Amin et al. (2015) and Sharma et al. (2022) in lentil and Hasan et al.…”

Section: Discussionsupporting

confidence: 83%

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Frequency and spectrum of M2 mutants and genetic variability in cyto-agronomic characteristics of fenugreek induced by caffeine and sodium azide

Naaz

Choudhary²,

Sharma³

et al. 2023

Front. Plant Sci.

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Trigonella foenum graecum L. (Fenugreek) is a valuable medicinal plant cultivated for decades for its therapeutic characteristics. Still no pronounced improvement concerning wild form was accomplished as it is a self-pollinating crop. Induced mutagenesis is encouraged as a remarkable tool on this plant to circumvent the genetic bottleneck of cultivated germplasms. As a result, novel allelomorphic combinations for short-term agronomic attributes were developed. Fenugreek cultivar Pusa Early Bunching, selected for the present experiment, was mutagenized with five doses (0.2%, 0.4%, 0.6%, 0.8%, and 1.0%) of caffeine and sodium azide (SA) to evaluate its impact on the qualitative and quantitative traits of M1 and M2 generation conducted in a Complete Randomized Block Design (CRBD), replicated five times during 2019–2020 and 2020–2021, respectively. The frequency of induced phenotypic variations was assessed in M2 progenies, resulting in the identification and isolation of a broad spectrum of mutants with altered phenotypes. Mutagenic effectiveness and efficiency were found to be maximum at lower concentrations of the mutagen treatments and highest in SA, followed by caffeine. Various morphological mutants with modified characters were observed at different concentrations in M2 generation. The spectrum of mutations was wider in SA than in caffeine, as caffeine produced 51 while SA produced 54 individual mutants under seven major categories. The maximum frequency of morphological mutants was associated with leaf, followed by plant size, plant growth habit, pod, seed size, seed shape, and seed color. Morphological and structural variations in the guard cells of stomata and seeds were observed through scanning electron microscopy. The variations created in the economically important traits may enrich the genetic diversity of this plant species. Moreover, these morphological mutants may serve as a source of elite genes in further breeding programs of fenugreek.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionsupporting

confidence: 89%

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Frequency and spectrum of M2 mutants and genetic variability in cyto-agronomic characteristics of fenugreek induced by caffeine and sodium azide

Naaz

Choudhary²,

Sharma³

et al. 2023

Front. Plant Sci.

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“…A notable increase in the mean number of branches was observed at lower concentrations of mutagens, consistent with previous findings in fenugreek, urdbean, and lentil ( Naaz et al, 2020 ; Goyal et al, 2022 ; Sharma et al, 2022 ). This increase in fertile branches per plant may be associated with reduced synthesis of strigolactones, influencing branching patterns.…”

Section: Discussionsupporting

confidence: 91%

“…Laggards, observed in some instances, are attributed to abnormal spindle fibers failing to bind and transport respective chromosomes to the polar region during meiosis ( Tarar and Dnyansagar, 1980 ). These findings align with previous research on various plant species such as T. foenum-graecum ( Choudhary et al, 2012 ; Naaz et al, 2023 ), C. annum ( Hasan et al, 2022 ), and L. culinaris ( Sharma et al, 2022 ), emphasizing the significance of chromosomal studies in understanding the complex effects of mutagens on chromosome behavior and structure during meiosis ( Figure 4 ).…”

Section: Discussionsupporting

confidence: 90%

Enhancing genetic variability in Trigonella species through sodium azide induction: morpho-physiological and chromosomal amelioration

Naaz,

Choudhary,

Hasan

et al. 2024

Front. Genet.

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Plant breeding, aimed at enhancing desired traits, depends on genetic diversity. Mutation breeding is a powerful method of rapidly expanding genetic diversity, facilitating crop improvement, and ensuring food security. In a recent study, researchers evaluated the genetic variability of Trigonella species using different doses of sodium azide (SA) (0.2%, 0.4%, 0.6%, 0.8%, and 1.0%) through morphological, physiological, and cytogenetic studies. Morphological variations were observed in cotyledonary leaves, vegetative leaves, and overall plant growth and habit. Several quantitative parameters, such as plant height, fertile branches per plant, pods per plant (or clusters), seeds per pod, and seed yield, increased when treated with 0.2% and 0.4% SA compared to the control. Furthermore, the total chlorophyll content and carotenoids increased in the sample treated with 0.2% SA over the control but decreased with higher concentrations. Scanning electron microscopy revealed that stomatal aperture and seed dimensions increased at lower concentrations of sodium azide treatment. The study found a positive correlation between the different parameters studied in the Trigonella species, as indicated by high r-values. Based on their findings, it was concluded that the genotype of fenugreek can be improved by using 0.2% and 0.4% concentrations of sodium azide. However, the evaluation of observed variants in successive generations is a critical and necessary process to validate their potential as keystones for crop genetic improvements.

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Turmeric (Curcuma longa L.) tends to reduce the toxic effects of nickel (II) chloride in Allium cepa L. roots

Macar

Çavuşoğlu

et al. 2022

Environ Sci Pollut Res

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Induction of chromosomal and morphological amelioration in lentil (Lens culinaris Medik.) mutagenized population developed through chemical mutagenesis

Cited by 5 publications

References 16 publications

Frequency and spectrum of M2 mutants and genetic variability in cyto-agronomic characteristics of fenugreek induced by caffeine and sodium azide

Frequency and spectrum of M2 mutants and genetic variability in cyto-agronomic characteristics of fenugreek induced by caffeine and sodium azide

Enhancing genetic variability in Trigonella species through sodium azide induction: morpho-physiological and chromosomal amelioration

Turmeric (Curcuma longa L.) tends to reduce the toxic effects of nickel (II) chloride in Allium cepa L. roots

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