Induction of chronic relapsing experimental allergic encephalomyelitis in Biozzi mice

Baker, David; O'Neill, J.K.; Gschmeissner, Steve; Wilcox, C.E.; Butter, C.; Turk, J.L.

doi:10.1016/0165-5728(90)90019-j

Cited by 189 publications

(182 citation statements)

References 17 publications

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“…Immunocytochemical studies of CR-EAE in the guinea pig have demonstrated T cells in the spinal cord (Traugott et al, 1982;Antoniou et al, 1987a;Butter et al, 1989). In mice, immunocytochemical studies demonstrated CD4 + T cells in the CNS in relapses of CR-EAE (Traugott et al, 1986;Baker et al, 1990) and radio-labelling has demonstrated passively transferred cells in the nervous system, especially in perivascular cuffs, in CR-EAE in SJL/J mice (Cross et al, 1990). These studies, together with the present study which clearly demonstrates an influx of CD4 + T cells into the CNS in the second episode of CR-EAE, suggest that CD4 + T cells, the effector cells in acute EAE (Pettinelli and McFarlin, 1981;Zamvil et al, 1985), continue to be important in relapses of CR-EAE.…”

Section: Discussionmentioning

confidence: 99%

“…In the guinea pig (Traugott et al, 1982;Butter et al, 1989) and mouse (Traugott et al, 1986;Baker et al, 1990) models of CR-EAE, immunocytochemical studies have demonstrated T cells and macrophages in the central nervous system (CNS). In CR-EAE induced in the Lewis rat by treatment with low dose CsA, Polman et al (1988) found T cells and macrophages in the CNS 50 days after inoculation.…”

Section: Introductionmentioning

confidence: 99%

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Inflammatory cells, microglia and MHC class II antigen-positive cells in the spinal cord of Lewis rats with acute and chronic relapsing experimental autoimmune encephalomyelitis

McCombe

Jersey

Pender

1994

Journal of Neuroimmunology

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Chronic relapsing experimental autoimmune encephalomyelitis (CR-EAE) was induced in Lewis rats by inoculation with guinea pig spinal cord and adjuvants and treatment with low dose cyclosporin A (CsA). Acute EAE was induced by the same method without CsA treatment. Immunocytochemistry and flow cytometry were used to assess inflammatory cells and MHC class II (Ia) antigen expression in the central nervous system of these rats. The inflammatory infiltrate was composed mainly of CD4 + T cells and macrophages, and αβ T cells constituted about 65% of the CD2 + T cells. After recovery from acute EAE and during the first remission of CR-EAE, the number of T cells was significantly less than in the preceding episodes. The number of T cells was higher in the second episode of CR-EAE than in the first remission. Throughout the course of CR-EAE, the majority of the CD2 + T cells were CD45RC −. The ratio of IL-2R + cells to CD2 + cells ranged from 10.5 to 24.0%. The ratio of CD4 + T cells to B cells was lower in the later episodes of CR-EAE than in the first episode. Ia antigen was expressed on infiltrating round cells at all stages of CR-EAE and on microglial cells (identified by dendritic morphology) with increasing intensity throughout the course of CR-EAE. With flow cytometry, the number of Ia + cells obtained from the spinal cord rose throughout the course of CR-EAE. The number of FSC low OX1 low cells, which we consider represent microglia, also increased during the course of CR-EAE.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Inflammatory cells, microglia and MHC class II antigen-positive cells in the spinal cord of Lewis rats with acute and chronic relapsing experimental autoimmune encephalomyelitis

McCombe

Jersey

Pender

1994

Journal of Neuroimmunology

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“…EAE was induced by immunization with spinal cord homogenate or myelin oligodendrocyte glycoprotein (MOG) [8][9][10][11][12][13][14][15][16][17][18][19][20][21] in CFA as described [27,28]. Deep and superficial CLN were also isolated from CCR7-deficient (n=4) and wild-type mice (n=4) [29] with chronic EAE, obtained from stock bred at the animal facility of the Max Delbrück Center for Molecular Medicine (Berlin, Germany).…”

Section: Eae Tissuesmentioning

confidence: 99%

Brain antigens in functionally distinct antigen-presenting cell populations in cervical lymph nodes in MS and EAE

et al. 2008

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Drainage of central nervous system (CNS) antigens to the brain-draining cervical lymph nodes (CLN) is likely crucial in the initiation and control of autoimmune responses during multiple sclerosis (MS). We demonstrate neuronal antigens within CLN of MS patients. In monkeys and mice with experimental autoimmune encephalomyelitis (EAE) and in mouse models with non-inflammatory CNS damage, the type and extent of CNS damage was associated with the frequencies of CNS antigens within the cervical lymph nodes. In addition, CNS antigens drained to the spinal-cord-draining lumbar lymph nodes. In human MS CLN, neuronal antigens were present in pro-inflammatory antigen-presenting cells (APC), whereas the majority of myelin-containing cells were anti-inflammatory. This may reflect a different origin of the cells or different drainage mechanisms. Indeed, neuronal antigen-containing cells in J Mol Med (2009) human CLN did not express the lymph node homing receptor CCR7, whereas myelin antigen-containing cells in situ and in vitro did. Nevertheless, CLN from EAE-affected CCR7-deficient mice contained equal amounts of myelin and neuronal antigens as wild-type mice. We conclude that the type and frequencies of CNS antigens within the CLN are determined by the type and extent of CNS damage. Furthermore, the presence of myelin and neuronal antigens in functionally distinct APC populations within MS CLN suggests that differential immune responses can be evoked.

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“…(University College London, London, U.K.) and fed rat-mouse expanded diet and water ad libitum. Mice aged between 6 and 8 wk old were injected s.c. in the flank with 1 mg mouse spinal cord homogenate (SCH) in IFA supplemented with 60 g Mycobacterium tuberculosis H37Ra and Mycobacterium butyricum on days 0 and 7 as described previously (12). Animals were monitored daily and clinical signs scored as follows: 0, normal; 1, flaccid tail; 2, impaired righting reflex; 3, partial paralysis; 4, complete paralysis.…”

Section: Animals and Disease Inductionmentioning

confidence: 99%

Different Therapeutic Outcomes in Experimental Allergic Encephalomyelitis Dependant Upon the Mode of Delivery of IL-10: A Comparison of the Effects of Protein, Adenoviral or Retroviral IL-10 Delivery into the Central Nervous System

Croxford¹,

Feldmann

Chernajovsky

et al. 2001

The Journal of Immunology

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Experimental allergic encephalomyelitis (EAE) is a CNS autoimmune disease mediated by the action of CD4+ T cells, macrophages, and proinflammatory cytokines. IL-10 is a cytokine shown to have many anti-inflammatory properties. Studies have shown both inhibition and exacerbation of EAE after systemic IL-10 protein administration. We have compared the inhibitory effect in EAE of Il10 gene delivery in the CNS. Fibroblasts transduced with retroviral vectors expressing IL-10 could inhibit EAE. This was not associated with a prevention of cellular recruitment but an alteration in their phenotype, notably an increase in the numbers of CD8+ T and B cells. In marked contrast, CNS delivery of adenovirus coding for mouse IL-10 or IL-10 protein performed over a wide dose range failed to inhibit disease, despite producing similar or greater amounts of IL-10 protein. Thus the action of IL-10 may differ depending on the local cytokine microenvironment produced by the gene-secreting cell types.

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Induction of chronic relapsing experimental allergic encephalomyelitis in Biozzi mice

Cited by 189 publications

References 17 publications

Inflammatory cells, microglia and MHC class II antigen-positive cells in the spinal cord of Lewis rats with acute and chronic relapsing experimental autoimmune encephalomyelitis

Inflammatory cells, microglia and MHC class II antigen-positive cells in the spinal cord of Lewis rats with acute and chronic relapsing experimental autoimmune encephalomyelitis

Brain antigens in functionally distinct antigen-presenting cell populations in cervical lymph nodes in MS and EAE

Different Therapeutic Outcomes in Experimental Allergic Encephalomyelitis Dependant Upon the Mode of Delivery of IL-10: A Comparison of the Effects of Protein, Adenoviral or Retroviral IL-10 Delivery into the Central Nervous System

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