Induction of Class-1 Non-symbiotic Hemoglobin Genes by Nitrate, Nitrite and Nitric Oxide in Cultured Rice Cells

Ohwaki, Yoshinari; Kawagishi-Kobayashi, Makiko; Wakasa, K.; Fujihara, Shinsuke; Yoneyama, T.

doi:10.1093/pcp/pci030

Cited by 82 publications

(59 citation statements)

References 44 publications

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“…Hb induction is observed in response to nitrate (Nie and Hill, 1997), nitrite and NO treatment (Ohwaki et al, 2005). It is known that in hypoxic conditions, nitrate reductase is usually induced and nitrite is accumulated (Morard et al, 2004).…”

Section: I T O C Ho N D R I a U N D E R H Y P O X I Amentioning

confidence: 99%

The Haemoglobin/Nitric Oxide Cycle: Involvement in Flooding Stress and Effects on Hormone Signalling

Igamberdiev¹,

Baron²,

Manac'h-Little³

et al. 2005

Annals of Botany

135

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Background Class 1 haemoglobins (Hbs) are induced in plant cells under hypoxic conditions. They have a high affinity for oxygen, which is two orders of magnitude lower than that of cytochrome oxidase, permitting the utilization of oxygen by the molecule at extremely low oxygen concentrations. Their presence reduces the levels of nitric oxide (NO) that is produced from nitrate ion during hypoxia and improves the redox and energy status of the hypoxic cell.Scope The mechanism by which Hb interacts with NO under hypoxic conditions in plants is examined, and the effects of Hb expression on metabolism and signal transduction are discussed.Conclusions The accumulated evidence suggests that a metabolic pathway involving NO and Hb provides an alternative type of respiration to mitochondrial electron transport under limited oxygen. Hb in hypoxic plants acts as part of a soluble, terminal, NO dioxygenase system, yielding nitrate ion from the reaction of oxyHb with NO. NO is mainly formed due to anaerobic accumulation of nitrite. The overall reaction sequence, referred to as the Hb/NO cycle, consumes NADH and maintains ATP levels via an as yet unknown mechanism. Hb gene expression appears to influence signal transduction pathways, possibly through its effect on NO, as evidenced by phenotypic changes in normoxic Hb-varying transgenic plants. Ethylene levels are elevated when Hb gene expression is suppressed, which could be a factor leading to root aerenchyma formation during hypoxic stress.

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Section: I T O C Ho N D R I a U N D E R H Y P O X I Amentioning

confidence: 99%

The Haemoglobin/Nitric Oxide Cycle: Involvement in Flooding Stress and Effects on Hormone Signalling

Igamberdiev¹,

Baron²,

Manac'h-Little³

et al. 2005

Annals of Botany

135

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“…Table 1 shows that Hb transcripts coexist in rice growing under cold, drought and salt stress conditions. Also, Ohwaki and co-workers 83 reported that nshb1 and nshb2 are induced by nitrate, nitrite and NO in cultured rice cells. These observations indicate that rice hb genes response to a variety of stress conditions.…”

Section: Molecular Biology Of Rice Hemoglobinsmentioning

confidence: 97%

Rice (Oryza) hemoglobins

2014

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Hemoglobins (Hbs) corresponding to non-symbiotic (nsHb) and truncated (tHb) Hbs have been identified in rice ( Oryza). This review discusses the major findings from the current studies on rice Hbs. At the molecular level, a family of the nshb genes, consisting of hb1, hb2, hb3, hb4 and hb5, and a single copy of the thb gene exist in Oryza sativa var. indica and O. sativa var. japonica, Hb transcripts coexist in rice organs and Hb polypeptides exist in rice embryonic and vegetative organs and in the cytoplasm of differentiating cells. At the structural level, the crystal structure of rice Hb1 has been elucidated, and the structures of the other rice Hbs have been modeled. Kinetic analysis indicated that rice Hb1 and 2, and possibly rice Hb3 and 4, exhibit an extremely high affinity for O 2, whereas rice Hb5 and tHb possibly exhibit a low to moderate affinity for O 2. Based on the accumulated information on the properties of rice Hbs and data from the analysis of other plant and non-plant Hbs, it is likely that Hbs play a variety of roles in rice organs, including O 2-transport, O 2-sensing, NO-scavenging and redox-signaling. From an evolutionary perspective, an outline for the evolution of rice Hbs is available. Rice nshb and thb genes vertically evolved through different lineages, rice nsHbs evolved into clade I and clade II lineages and rice nshbs and thbs evolved under the effect of neutral selection. This review also reveals lacunae in our ability to completely understand rice Hbs. Primary lacunae are the absence of experimental information about the precise functions of rice Hbs, the properties of modeled rice Hbs and the cis-elements and trans-acting factors that regulate the expression of rice hb genes, and the partial understanding of the evolution of rice Hbs.

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“…It has been reported that nsHb1 genes are induced by osmotic stress and high salinity (Taylor et al, 1994;Trevaskis et al, 1997;Lira-Ruan et al, 2001;Zhao et al, 2008;Almada et al, 2013), by treatments with nitrate, nitrite, nitric oxide, salicylic acid, methyl jasmonic acid, and H 2 O 2 (Wang et al, 2000;Sakamoto et al, 2004;Ohwaki et al, 2005;Qu et al, 2006;Sasakura et al, 2006), as well as by deficiencies in minerals such as P, K, and Fe (Wang et al, 2003). ZmHb mRNA levels in maize seedlings are induced by high-salt and osmotic stresses in addition to hypoxic stress (Zhao et al, 2008).…”

Section: Regulation By Osmotic Stress and Mineralsmentioning

confidence: 99%

“…Hemoglobin is also induced by nitrate ions in aleurone layers and in Arabidopsis roots (Nie and Hill, 1997;Wang et al, 2000). Two nsHb1 genes, ORYsa GLB1a and ORYsa GLB1b, were strongly induced by nitrate, nitrite, and nitric oxide (Ohwaki et al, 2005). The induction of the Hb gene by hypoxia is linked to a decrease in ATP levels and is mediated by Ca 2+ (Hebelstrup et al, 2007).…”

Section: Regulation By Osmotic Stress and Mineralsmentioning

confidence: 99%

Non-symbiotic hemoglobin and its relation with hypoxic stress

Riquelme¹,

Hinrichsen

2015

Chilean J. Agric. Res.

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Today we know that several types of hemoglobins exist in plants. The symbiotic hemoglobins were discovered in 1939 and are only found in nodules of plants capable of symbiotically fixing atmospheric N. Another class, called non-symbiotic hemoglobin, was discovered 32 yr ago and is now thought to exist throughout the plant kingdom, being expressed in different organs and tissues. Recently the existence of another type of hemoglobin, called truncated hemoglobin, was demonstrated in plants. Although the presence of hemoglobins is widespread in the plant kingdom, their role has not yet been fully elucidated. This review discusses recent findings regarding the role of plant hemoglobins, with special emphasis on their relationship to plants adaptation to hypoxia. It also discusses the role of nitric oxide in plant cells under hypoxic conditions, since one of the functions of hemoglobin appears to be modulating nitric oxide levels in the cells.

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Induction of Class-1 Non-symbiotic Hemoglobin Genes by Nitrate, Nitrite and Nitric Oxide in Cultured Rice Cells

Cited by 82 publications

References 44 publications

The Haemoglobin/Nitric Oxide Cycle: Involvement in Flooding Stress and Effects on Hormone Signalling

The Haemoglobin/Nitric Oxide Cycle: Involvement in Flooding Stress and Effects on Hormone Signalling

Rice (Oryza) hemoglobins

Non-symbiotic hemoglobin and its relation with hypoxic stress

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