Induction of collagenase and prostaglandin synthesis in synovial fibroblasts treated with monosodium urate crystals

McMillan, R. M.; Vater, Carol A.; Hasselbacher, Peter; Hahn, J; Harris, Edward D.

doi:10.1111/j.2042-7158.1981.tb13809.x

Cited by 29 publications

(20 citation statements)

References 12 publications

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“…For example, several groups have shown that both phorbol esters (47)(48)(49) and urate crystals (48,50) induce enhanced expression of collagenase in cultured rabbit synovial fibroblasts through a mechanism(s) that leads to enhanced translatable collagenase mRNA.…”

Section: Discussionmentioning

confidence: 99%

Stimulation of in vitro human skin collagenase expression by platelet-derived growth factor.

Bauer¹,

Cooper²,

Huang³

et al. 1985

Proc. Natl. Acad. Sci. U.S.A.

190

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Platelet-derived growth factor (PDGF) is both chemoattractant and mitogenic for stromal cells. Here, we examined the effects of PDGF on collagenase expression by normal human skin fibroblasts. Culturing cells for 24 hr in the presence ofPDGF at 0-180 ng/ml resulted in a dose-dependent, saturable increase in collagenase activity in the culture medium that was paralleled by equal increases in immunoreactive collagenase protein, suggesting enhanced synthesis of a catalytically unaltered enzyme. The specificity of this effect was demonstrated by comparing the collagenase-stimulatory effect with that on total protein synthesis and DNA synthesis. Under in vitro conditions that produced a 2.5-fold increase in collagenase synthesis, there was an =20% increase in total protein synthesis and no change in DNA synthesis. In addition, platelet factor 4, another platelet-derived protein, caused a <20% increase in collagenase expression. In time-course studies, stimulation of collagenase synthesis was first observed 8-10 hr after exposure to the growth factor. Conversely, when cells were primed with PDGF for =24 hr and the stimulator was then removed, an increased rate of synthesis was seen for an additional =6 hr, after which the rate reverted to control levels. Since the kinetic data suggested a possible pretranslational effect, fibroblasts cultured with PDGF were used to prepare mRNA. In cell-free translation, total protein synthesis was essentially unaltered; however, the growth factor caused a >2-fold increase in translatable collagenase mRNA. The data suggest that PDGF specifically modulates collagenase synthesis, possibly through a series of events that lead to increased transcription or preferential translation of collagenase mRNA.

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Section: Discussionmentioning

confidence: 99%

Stimulation of in vitro human skin collagenase expression by platelet-derived growth factor.

Bauer¹,

Cooper²,

Huang³

et al. 1985

Proc. Natl. Acad. Sci. U.S.A.

190

View full text Add to dashboard Cite

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“…A feature common to most, if not all, stimuli of collagenase synthesis is membrane perturbation, such as occurs during cell fusion (38) or phagocytosis (16) or more subtly, via specific membrane receptors (39). After this initial event, but preceding the initiation of transcription, there may be a series of changes marking an "activated" cell, as has been described for neutrophils (40).…”

Section: Discussionmentioning

confidence: 99%

“…In general these include a change in membrane potential, mobilization of cellular phospholipids, an influx of Ca2+, rearrangement of the cytoskeleton, and the generation of cAMP. Phospholipid metabolism (15,16,41), redistribution of microfilaments (42), and an increase in cAMP (15,43) have been associated with increased production of collagenase by synovial cells.…”

Section: Discussionmentioning

confidence: 99%

“…Monolayer cultures of normal rabbit synovial fibroblasts were established as described (15,19). For experiments, cells were grown to confluence in 100-mm-or 150-mm-diameter culture dishes and then placed in serumfree Dulbecco's modified Eagle's medium/0.2% lactalbumin hydrolysate in the presence or absence of crystals of monosodium urate monohydrate at 350 pug/ml (16,19). At appropriate times, medium was harvested and assayed for collagenase enzyme activity or for detection of collagenase protein by immunoblotting techniques (see below).…”

Section: Methodsmentioning

confidence: 99%

“…To study the mechanisms governing the control of collagenase synthesis by synovial cells, we have developed a model system that uses monolayer cultures of rabbit synovial cells. These cells secrete negligible quantities of collagenase unless they are stimulated with agents such as the tumor promoter phorbol myristate acetate (15) or crystals of monosodium urate monohydrate (16). Collagenase activity, measured in a fibril assay (17), is detectable in culture medium -24 hr after addition of these stimuli and by [30][31][32][33][34][35][36] hr is present in levels comparable with those achieved in primary cultures of human rheumatoid synovial cells (18).…”

mentioning

confidence: 99%

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Isolation of a collagenase cDNA clone and measurement of changing collagenase mRNA levels during induction in rabbit synovial fibroblasts.

Gross¹,

Sheldon²,

Fletcher³

et al. 1984

Proc. Natl. Acad. Sci. U.S.A.

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To facilitate our studies on the mechanisms controlling collagenase production at a molecular level in rabbit synovial fibroblasts (22) or nonimmune IgG was added to the supernatant and the mixture was incubated at 4°C overnight. The antigen-antibody complexes were isolated with protein A-Sepharose. After 1 hr, the Sepharose beads were washed twice with 1 M NaCl/5 mM EDTA/50 mM Tris HCl, pH 7.5/0.5% Triton X-100, twice with 0.1 M NaCI/1 mM EDTA/10 mM Tris HCl, pH 7.5/1% Triton X-100/0.5% deoxycholate (Sigma)/0.1% NaDodSO4, once with 0

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Proline‐rich tyrosine kinase 2 and Src kinase signaling transduce monosodium urate crystal–induced nitric oxide production and matrix metalloproteinase 3 expression in chondrocytes

Liu

Lioté

Rose

et al. 2004

Arthritis & Rheumatism

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Objective. Articular deposition of monosodium urate monohydrate (MSU) crystals may promote cartilage and bone erosion. Therefore, the aim of this study was to determine how MSU crystals stimulate chondrocytes. Conclusion. In chondrocytes, MSU crystals activate a signaling kinase cascade typically employed by adhesion receptors that involves upstream Src and FAK family activation and downstream p38 activation. In this cascade, Pyk-2, Src, and p38 kinases transduce MSU-induced NO production and MMP-3 expression. Our results identify Pyk-2 and c-Src as novel sites for potential therapeutic intervention in cartilage degradation in chronic gout.

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Induction of collagenase and prostaglandin synthesis in synovial fibroblasts treated with monosodium urate crystals

Cited by 29 publications

References 12 publications

Stimulation of in vitro human skin collagenase expression by platelet-derived growth factor.

Stimulation of in vitro human skin collagenase expression by platelet-derived growth factor.

Isolation of a collagenase cDNA clone and measurement of changing collagenase mRNA levels during induction in rabbit synovial fibroblasts.

Proline‐rich tyrosine kinase 2 and Src kinase signaling transduce monosodium urate crystal–induced nitric oxide production and matrix metalloproteinase 3 expression in chondrocytes

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