Induction of cyclooxygenase‐2 mRNA and protein expression by dentin bonding agents in human gingival fibroblasts

Abstract

An ideal dentin bonding agent should be nonirritating to surrounding tissues. Unfortunately, all histological investigations have demonstrated that dentin bonding agents can induce mild to severe inflammatory alterations. However, there is little information on the precise mechanisms about dentin bonding agents-induced inflammatory reaction. Cyclooxygenase-2 (COX-2) is an inducible enzyme believed to be responsible for prostaglandin synthesis at the site of inflammation. The aim of the present study was to investigate the effects of three dentin bonding agents, Clearfil SE Bond, Prime & Bond NT, and Single Bond on the expression of COX-2 mRNA gene and protein in cultured human gingival fibroblasts. The exposure of quiescent human gingival fibroblasts to dentin bonding agents resulted in the induction of COX-2 mRNA expression. The investigations of the time-dependent on COX-2 mRNA expression in dentin bonding agent-treated human gingival fibroblasts revealed different patterns. The influence of COX-2 mRNA depended on the tested materials. In addition, all dentin bonding agents also induced COX-2 protein expression in human gingival fibroblasts. Taken together, the activation of COX-2 expression may be one of the potential mechanisms of dentin bonding agent-induced gingival inflammation.

Induction of heme oxygenase‐1 expression by root canal sealers in human gingival fibroblasts is augmented by oxidative stress

“…HGFs were cultured using an explant technique as described previously 16, 17. Gingival connective tissues from crown‐lengthening surgery were used to culture gingival fibroblasts.…”

“…HGFs were cultured using an explant technique as described previously. 16,17 Gingival connective tissues from crownlengthening surgery were used to culture gingival fibroblasts. All patients were duly informed of the nature and extent of the study, and their informed consent was obtained according to the Helsinki Declaration.…”

The regulation of cytotoxicity and cyclooxygenase‐2 expression by 2‐hydroxy‐ethyl methacrylate in human osteoblasts are related to intracellular glutathione levels

“…Previous studies have shown that COX‐2, a key enzyme leading to the inflammatory reaction of tissues, was induced by resin‐based dental materials (Huang et al . , Huang & Chang , ). These results may provide a molecular basis for what was observed in locally inflamed tissues after the application of resin‐based root canal sealers and dentine bonding agents, although it is uncertain whether the resin monomers amongst the ingredients of the commercial products were major causative agents that provoked inflammation.…”

The importance of cyclooxigenase in dentistry

“…TEGDMA and BisGMA, both monomers of composite resin, induce the overexpression of COX-2 and PGE2 in dental pulp cells 101,102 . Dental bonding agents induce COX-2 overexpression in gingival fibroblasts 103 . Tannin fluoride suppresses COX-2 and PGE2 expression induced by glass ionomer cement in dental pulp cells 104 .…”