Induction of CYP1A by carbofuran in primary culture of fish hepatocytes

“…Our laboratory reported that cytochrome P4501a might be involved in degradation of this pesticide in hepatocytes [11,12,13]. The mechanism of degradation is also associated with the induction of several critical pathways including, PI3 kinase, PKC, and HSP70 [12].…”

Section: Introductionmentioning

confidence: 97%

Carbofuran Abrogates The Membrane Ca++-ATPase Activity In Liver: The Role Of Cholesterol

Ghosh¹,

Shukla²,

Ray³

2014

JPGR

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Carbofuran is a broad spectrum pesticide used in agricultural fields and domestic places throughout the world. It is one of the deadly toxic carbamate pesticide that kills the pest by inhibiting the crucial enzyme of nervous system known as acetyl cholinesterase. In the present study, we report how carbofuran increases the different spectrum of cholesterols, including free cholesterol and esterified cholesterol in the fish hepatocytes. It is observed that induced-cholesterol can inhibit the enzymatic activity such as Ca++-ATPase, which is a critical protein for maintaining the calcium homoeostasis in the cellular microenvironment. Carbofuran integrates into human body through foods and drinks. As trace of carbofuran is identified in our daily food and drinks, we examined the homology of Ca++-ATPase between the fish and human, so our data can illuminate the effects of carbofuran on this crucial enzyme. While studying the homology with the help of bioinformatics, we recognized that there is around 70% homology in the protein sequence of Ca++-ATPase between fish Heteropneustes fossilis and human (Homo sapiens), which appears as sufficient to simulate our fish-model data in human. This study demonstrates that carbofuran affects our day-to-day life by inhibiting Ca++-ATPase through modulation of lipid synthesis, a critical regulatory system that controls overall homeostasis in our body.

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“…Isolation of hepatocytes from catfish liver and their culture were done following the methods routinely used in our laboratory (Ghosh et al 2000).…”

Section: Culture Of Catfish Hepatocytesmentioning

confidence: 99%

“…The procedure of EROD activity assay was followed as described before (Ghosh et al 2000). Table 1 Composition and amount of reagents used to assay the PKC.…”

Section: Assay Of Ethoxyresorufin-o-deethylase Activity (Erod) Activitymentioning

confidence: 99%

Regulation of cytochrome P4501A by protein kinase C: the role of heat shock protein70

Ghosh

Ray

2011

J. Cell Commun. Signal.

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Carbofuran is a pesticide, which is used throughout the world as a nematicide and an acaricide. This pesticide integrates into living organisms through aquatic ecosystem. In earlier report, we had demonstrated that cytochrome P4501A was induced in cultured catfish hepatocytes in response to carbofuran, which might be responsible for the detoxification of this pesticide. As the underlying signaling mechanism associated with induction and regulation of cytochrome P4501A has not yet been well defined, we therefore in the present study have investigated to identify the regulatory network of cytochrome P4501A in catfish liver or cultured hepatocytes by targeting several key signaling molecules such as phosphatidyl inositol (PI) or protein kinase C (PKC), which are critical molecules for many important pathways. PKC and heat shock protein70 (HSP70) have been shown to be induced in response to carbofuran in catfish hepatocytes. Results also indicate that induction of CYP1A is modulated by HSP70 and PKC in fish hepatocytes. Thus our data shed light on the regulation of EROD activity, which has been used as a bio-monitoring tool for measuring aquatic pollution.

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Induction of CYP1A by carbofuran in primary culture of fish hepatocytes

Cited by 19 publications

References 21 publications

Affinity membrane chromatography: relationship of dye-ligand type to surface polarity and their effect on lysozyme separation and purification

Affinity membrane chromatography: relationship of dye-ligand type to surface polarity and their effect on lysozyme separation and purification

Carbofuran Abrogates The Membrane Ca++-ATPase Activity In Liver: The Role Of Cholesterol

Regulation of cytochrome P4501A by protein kinase C: the role of heat shock protein70

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