Induction of Cytochrome P450 1A (CYPl A) in Fish: A Biomarker for Environmental Pollution

Nilsen, Bente M.; Berg, Karin; GoksoØr, Anders

doi:10.1385/0-89603-519-0:423

Cited by 45 publications

(43 citation statements)

References 13 publications

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“…Quantification was assessed by enzyme-linked immunosorbent assay (ELISA) in the microssomal fraction of liver homogenates, according to Nilsen et al (1998) using a polyclonal antibody (CP226) against fish CYP1A (BIOSENSE). CYP1A extraction and quantification was performed in triplicate to determine the error of procedure.…”

Section: Biomarker Analysesmentioning

confidence: 99%

Biochemical endpoints on juvenile Solea senegalensis exposed to estuarine sediments: the effect of contaminant mixtures on metallothionein and CYP1A induction

et al. 2009

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Juvenile Solea senegalensis were exposed to fresh sediments from three stations of the Sado estuary (Portugal) in 28-day laboratory assays. Sediments revealed distinct levels of total organic matter, fine fraction, redox potential, trace elements (arsenic, cadmium, chromium, copper, nickel, lead and zinc) and organic contaminants (polycyclic aromatic hydrocarbons, polychlorinated biphenyls and a pesticide: dichloro diphenyl trichloroethane). Organisms were surveyed for contaminant bioaccumulation and induction of two hepatic biochemical biomarkers: metallothionein (MT) and cytochrome P450 (CYP1A), as potential indicators of exposure to metallic and organic contaminants, respectively. Using an integrative approach it was established that, although bioaccumulation is in general accordance with sediment contamination, lethality and biomarker responses are not linearly dependent of the cumulative concentrations of sediment contaminants but rather of their bioavailability and synergistic effects in organisms. It is concluded that metals and organic contaminants modulate both MT and CYP1A induction and it is suggested that reactive oxygen species may be the link between responses and effects of toxicity.

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Section: Biomarker Analysesmentioning

confidence: 99%

Biochemical endpoints on juvenile Solea senegalensis exposed to estuarine sediments: the effect of contaminant mixtures on metallothionein and CYP1A induction

et al. 2009

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“…Another fraction of the liver samples was stored at -80°C for subsequent analysis of the CYP1A contents in the liver using a method described elsewhere by Nielsen et al (1998).…”

Section: Somatic Indicesmentioning

confidence: 99%

“…CYP1A) based mixed-function oxygenases (MFOs) have a key role in the biotransformation of many xenobiotics in vertebrates and can be readily measured in livers of fish (Chen et al 2001). CYP1A enzyme induction is generally used as a useful and sensitive biomarker of contaminant exposure, and have been used to assess the impacts of pulp mill effluents in fish species (Nielsen et al 1998). Changes in steroid concentrations have been used as biomarker to assess exposure effects to environmental contamination (Ishibashi et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Effects of ECF-Kraft pulp mill effluent treated with fungi (Rhizopus oryzae) on reproductive steroids and liver CYP1A of exposed goldfish (Carassius auratus)

et al. 2009

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The toxicity of bleached Kraft pulp mill effluents (BKME) is usually attributed to chemical compounds which are produced and released throughout various stages of pulp and paper production. The main objective of the present work was to detect sub-lethal responses of goldfish (Carassius auratus) to secondary treated BKME which was treated with Rhizopus oryzae. A total of 96 carps (C. auratus; 11 +/- 3 g) were exposed to different concentrations of the post-treated effluent (0, 1, 10, 25, 50, and 100%), in 28 days semi-static tests. Several biomarkers were then evaluated to assess the toxicological effects: induction of CYP1A (metabolic processes of organic compounds in liver), change in steroid profiles (11-Ketotestosterone, 17beta-estradiol), histopathology of liver and gonads and somatic indices (GSI, HSI) for endocrine disruption and other physiological disturbances. The most significant results show an induction of CYP1A in both sexes and a decrease of 17beta-estradiol concentrations in females. Histopathological changes such as liver tissue degeneration were observed in fish exposed to 50 and 100% of the BKME. Although the BKME was biologically treated there are some chemical compounds in the effluent that are capable to affect fish physiology, however, a clear evidence for endocrine disruption was not found.

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“…Fish cytochrome P450-dependent monooxygenases play a crucial role in the metabolism of numerous endogenous and exogenous compounds. Especially, the CYP1A subfamily has a very important role in evaluating the consequences of environmental pollution created by carcinogenic and mutagenic PAHs and polychlorinated hydrocarbons as a biomonitoring tool for biological interpretation (Nilsen et al 1998;Sandvik et al 1997;Bucheli and Fent 1995;Fent 2004). Increased CYP1A and associated 7-ethoxyresorufin O-deethylase (EROD) activity in fish, especially in areas polluted by petroleum hydrocarbons, paper bleaching, and other industrial and city sewerage systems, are among the biological biomarkers most commonly used to observe environmental contamination occurring in the areas mentioned (Julkunen et al 1986;Stegeman 1987;Kirby et al 1999).…”

Section: Introductionmentioning

confidence: 99%

“…Increased CYP1A and associated 7-ethoxyresorufin O-deethylase (EROD) activity in fish, especially in areas polluted by petroleum hydrocarbons, paper bleaching, and other industrial and city sewerage systems, are among the biological biomarkers most commonly used to observe environmental contamination occurring in the areas mentioned (Julkunen et al 1986;Stegeman 1987;Kirby et al 1999). The measurement of EROD activity appears to be the most sensitive and the most widely used catalytic probe for determining the induction response of CYP1A in fish (Julkunen et al 1986;Stegeman and Lecht 1991;Bucheli and Fent 1995;Sandvik et al 1997;Nilsen et al 1998;Pacheco and Santos 1998;Kirby et al 1999;Arinc et al 2000;Arinc and Sen 1999;Fent 2004).…”

Section: Introductionmentioning

confidence: 99%

Determination of 7-ethoxyresorufin-o-deethylase (EROD) induction in leaping mullet (Liza saliens) from the highly contaminated Aliaga Bay, Turkey

Sen

Ulutaş

Tutuncu

et al. 2009

Environ Monit Assess

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Pollution of the aquatic environment is a global concern owing to the devastating effects of contaminants whose levels are growing at an alarming rate, and it has become a major threat for marine organisms, as well as to humans as consumers. This study has been carried out on leaping mullet obtained from Aliaga Bay, which is located on the west coast of the Aegean Sea near Izmir and hosts the world's fifth largest shipyard, plus a broad range of industrial activities, including an oil refinery and a paper factory. The waste from these industries, combined with municipal sewer discharges, is the main cause of pollution in this region. There is no national documentation or research on the determination of pollution resulting from the industrial activities in this area. In the present study, the degree of induction of CYP4501A-associated 7-ethoxyresorufin O-deethylase (EROD) activity and immunochemical detection of CYP1A1 in the liver of leaping mullet (Lisa saliens) were used as biomarkers for the assessment of polycyclic aromatic hydrocarbon (PAH)-type organic pollutants in Aliaga Bay. Mullet caught from different locations of the bay had approximately 52 times more EROD activity than the feral fish sampled from a clean reference site near Foca, Izmir. The results of this study indicate that Aliaga Bay is highly contaminated with PAH-type organic pollutants.

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Induction of Cytochrome P450 1A (CYPl A) in Fish: A Biomarker for Environmental Pollution

Cited by 45 publications

References 13 publications

Biochemical endpoints on juvenile Solea senegalensis exposed to estuarine sediments: the effect of contaminant mixtures on metallothionein and CYP1A induction

Biochemical endpoints on juvenile Solea senegalensis exposed to estuarine sediments: the effect of contaminant mixtures on metallothionein and CYP1A induction

Effects of ECF-Kraft pulp mill effluent treated with fungi (Rhizopus oryzae) on reproductive steroids and liver CYP1A of exposed goldfish (Carassius auratus)

Determination of 7-ethoxyresorufin-o-deethylase (EROD) induction in leaping mullet (Liza saliens) from the highly contaminated Aliaga Bay, Turkey

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