Induction of Cytokines in Human Peripheral Blood Mononuclear Cells by Mycoplasmas

Kita, Masakazu; Ohmoto, Yasukazu; Hirai, Yoshikatsu; Yamaguchi, Naohito; Imanishi, Jirô

doi:10.1111/j.1348-0421.1992.tb02048.x

Cited by 118 publications

(80 citation statements)

References 23 publications

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“…These inflammatory substances include interleukin-8, tumor necrosis factor-, macrophage inflammatory peptide-1 [Hardy et al, 2001], intercellular adhesion molecule-1 [Krunkosky et al, 2007], and regulated upon activation, normal T cells expression and secreted [Dakhama et al, 2003], among others. Tumor necrosis factor-has been observed to be induced by M. pneumoniae in vitro from an early period of investigation [Arai et al, 1990;Kita et al, 1992]. This cytokine, along with interleukin-8, must play a pivotal role in eliciting vasculitic/thrombotic vascular occlusion.…”

Section: Direct Mechanisms Of Vasculitic/thrombotic Vascular Occlusionmentioning

confidence: 99%

Mycoplasma pneumoniae as an Under- Recognized Agent of Vasculitic Disorders

Narita¹

2011

Advances in the Etiology, Pathogenesis and Pathology of Vasculitis

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Section: Direct Mechanisms Of Vasculitic/thrombotic Vascular Occlusionmentioning

confidence: 99%

Mycoplasma pneumoniae as an Under- Recognized Agent of Vasculitic Disorders

Narita¹

2011

Advances in the Etiology, Pathogenesis and Pathology of Vasculitis

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“…IL-8 levels in cell-free culture supernatants from transfected TMK-1 cells and homogenates of xenografts were assayed by enzymelinked immunosorbent assay (ELISA) as described previously (Kita et al, 1992;Kitadai et al, 1998b) using an IL-8 monoclonal antibody. The detection limit of the assay was 20 µg ml -1 , and inter-and intraassay variations were 4.1-4.5% and 7.9-9.3%, respectively.…”

Section: Enzyme-linked Immunosorbent Assay For Il-8 Proteinmentioning

confidence: 99%

Transfection of interleukin-8 increases angiogenesis and tumorigenesis of human gastric carcinoma cells in nude mice

et al. 1999

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SummaryThe growth and spread of tumour cells depends on adequate vasculature. We have previously reported that the expression of interleukin-8 (IL-8) directly correlates with the vascularity of human gastric carcinomas. To provide evidence for a causal role of IL-8 in angiogenesis and tumorigenicity of human gastric cancer, we used the lipofectin method to stably transfect the human TMK-1 gastric carcinoma cells (low endogenous IL-8) with an IL-8 expression vector or control vector. Transfection with IL-8 did not affect the proliferation of cultured cells, yet the culture supernatants of the transfected (but not control) cells stimulated proliferation of human umbilical vein endothelial cells. The IL-8-transfected and control cells were injected into the gastric wall of nude mice. IL-8-transfected cells produced rapidly growing, highly vascular neoplasms as compared to control cells. These results provide direct evidence for the role of IL-8 in the angiogenesis and tumorigenicity of human gastric carcinomas.

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“…Human airway cells co-cultured with the organism produce RANTES and TGFβ1 [32]. Peripheral blood mononuclear cells incubated with the organism released IL-2, TNFα, and IL-6 [33][34]. M. pneumoniae co-culture induced IL-8 and IL-1 production by human lung epithelial and monocytoid tumor lines [35][36].…”

Section: Discussionmentioning

confidence: 99%

Inhibition of message for FcεRI α chain blocks mast cell IL-4 production induced by co-culture with Mycoplasma pneumoniae

Luo

Dai

Duffy

et al. 2008

Microbial Pathogenesis

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We have previously described the activation of RBL-2H3 mast cells for IL-4 production by Mycoplasma pneumoniae but the mechanism remains unclear. M. pneumoniae binds eukaryotic cells primarily through sialoglycoproteins on the target cell surface. This study was undertaken to determine whether the sialated FcεRIα chain on RBL cells is important for M. pneumoniae-induced IL-4 production. We found that IgE-mediated IL-4 release by a series of RBL sublines correlated with the release induced by M. pneumoniae. Further, aggregation of FcγRII (CD32) in RBL cells using a monoclonal antibody inhibited both IgE-mediated and mycoplasma-induced IL-4 production, providing further evidence for an Fc receptor-mediated mechanism of activation. To examine the role of FcεRI in mycoplasma-induced IL-4 release, we created stably transfected RBL sublines using a vector expressing a short hairpin sequence designed to inhibit message for the FcεRI α chain. IgEinduced IL-4 production by the transfected sublines was reduced in similar proportion to the degree of message suppression. M. pneumoniae-induced IL-4 production in the four transfected sublines was completely blocked in contrast to results with the controls or parent RBL cells. We conclude that the heavily glycosylated FcεRI α chain is required for activation of mast cells for IL-4 production by M. pneumoniae.

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Induction of Cytokines in Human Peripheral Blood Mononuclear Cells by Mycoplasmas

Abstract: Various species of mycoplasmas were tested for their ability to induce cytokine production in human peripheral blood mononuclear cells (PBMC

Cited by 118 publications

References 23 publications

Mycoplasma pneumoniae as an Under- Recognized Agent of Vasculitic Disorders

Mycoplasma pneumoniae as an Under- Recognized Agent of Vasculitic Disorders

Transfection of interleukin-8 increases angiogenesis and tumorigenesis of human gastric carcinoma cells in nude mice

Inhibition of message for FcεRI α chain blocks mast cell IL-4 production induced by co-culture with Mycoplasma pneumoniae

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