Induction of cytosolic Glutathione S-transferases from Atlantic eel (Anguilla anguilla) after intraperitoneal treatment with polychlorinated biphenyls

López, Marcos Pérez; Valiñas, M.C Nóvoa; Riol, María Julia Melgar

doi:10.1016/s0048-9697(02)00130-4

Cited by 17 publications

(4 citation statements)

References 18 publications

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“…Several studies have been conducted to study the effect of Aroclor 1254 on vertebrates stress response enzymes. An induction of total glutathione S‐transferase (GST) activity against PCB and Aroclor 1254 was shown in the liver of European eel, Anguilla anguilla and rainbow trout, Oncorhynchus mykiss (Perez‐Lopez et al 2002a, 2002b). Additionally, Lee and Opanashuk (2004) described that the CuZn–SOD protein level increased in MN9D cells treated with Aroclor 1254 and the activation of oxidative stress‐related pathway by PCB.…”

Section: Discussionmentioning

confidence: 99%

Disk Abalone, Haliotis discus discus, CuZn–Superoxide Dismutase cDNA and its Transcriptional Induction by Aroclor 1254

Zoysa¹,

Ekanayake²,

Kang³

et al. 2009

J World Aquaculture Soc

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CuZn–superoxide dismutase (CuZn–SOD) is a key antioxidant enzyme playing a first line protective role against reactive oxygen species (ROS) by converting superoxide () into H2O2. The CuZn–SOD gene was isolated from a whole abalone cDNA library and denoted as aCuZn–SOD. The full‐length cDNA of aCuZn–SOD was 1021 bp, which contained 465‐bp open reading frame (ORF) coding 154 amino acids. It contained highly conserved CuZn–SOD signature motif 1 (45GFHVHQFGDNT55) and motif 2 (139GNAGGRQACGVI150). Also, amino acid residues identified as Cu (His47, His49, His64, and His121) and Zn (His64, His72, His81, and ASP84) metal‐binding sites were completely conserved in the aCuZn–SOD. The reverse transcription polymerase chain reaction (RT‐PCR) results showed that aCuZn–SOD mRNA was expressed constitutively in gill, mantle, gonad, abductor muscle, digestive tract, and hemocytes in a tissue‐specific manner. The aCuZn–SOD mRNA was significantly up‐regulated (P < 0.05) in gill and digestive tract tissues after Aroclor 1254 induction compared to untreated and methanol‐injected abalone groups, suggesting that abalone has a potential use in assessing the impact of marine pollutants with the application of gene expression concept. In addition, purified recombinant aCuZn–SOD fusion protein was shown to reduceradical generated by xanthine oxidase assay, showing CuZn–SOD is a functionally active antioxidant enzyme in disk abalone.

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Section: Discussionmentioning

confidence: 99%

Disk Abalone, Haliotis discus discus, CuZn–Superoxide Dismutase cDNA and its Transcriptional Induction by Aroclor 1254

Zoysa¹,

Ekanayake²,

Kang³

et al. 2009

J World Aquaculture Soc

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“…At present, no study detecting GST activity in subcellular fractions of bivalves has been found. According to Pérez López et al (2002), GST activity was determined in cytoplasm of Atlantic eel ( Anguilla anguilla ) liver. Gallagher et al (1991) reported that GST activity was detectable in liver and gill subcellular fractions of channel catfish ( Ictalurus punctatus ).…”

Section: Discussionmentioning

confidence: 99%

“…Currently, subcellular fractions of some aquatic animal tissues, for instance, microsomal, mitochondrial, and nuclear fractions, have been obtained by the methods of differential centrifugation (Barst et al, 2018; Kavun et al, 2020). Many important biochemical metabolic enzymes have been detected in subcellular fractions (Pérez López et al, 2002; Siebert et al, 2017), so subcellular fractions can participate in a variety of metabolic activities after PAH invasion. Furthermore, the structural damage of subcellular fractions would also be engendered during the process of PAH exposure, which is regarded as the effect of PAHs on biological macromolecular damage (Jin et al, 2016; Pangrekar et al, 1995; Yang et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Bioaccumulation, Detoxification, and Biological Macromolecular Damage of Benzo[a]pyrene in Exposure in Tissues and Subcellular Fractions of Scallop Chlamys farreri

Chen

Miao

et al. 2022

Enviro Toxic and Chemistry

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Because of the persistence and high toxicity of benzo[a]pyrene (B[a]P), the bioaccumulation and detoxification mechanisms of B[a]P have been studied extensively at the tissue level; but the data at the subcellular level in bivalves have not been reported. The present study was conducted to investigate the effects of B[a]P exposure on bioaccumulation, detoxification, and biomacromolecular damage in gills, digestive glands, and their subcellular fractions of the scallop Chlamys farreri. The subcellular fraction contains cytoplasm, mitochondria, microsome, nucleus, cell membrane, and overall organelle. The results demonstrated that B[a]P accumulation showed a clear time–dose effect. Based on the time‐dependent accumulation of B[a]P in subcellular fractions, we speculated that the intracellular migration order of B[a]P was cell membrane, organelle, and nucleus in turn. Considering the difference of B[a]P accumulation may be related to B[a]P metabolism, we have further confirmed that the activities of B[a]P metabolizing enzymes in scallop tissues and subcellular fractions were significantly tempted by B[a]P (p < 0.05), including 7‐ethoxyresorufin O‐deethylase (increased), glutathione‐S‐transferase (GST; decreased), and superoxide dismutase (increased). First, GST was detected in bivalve cytoplasm and microsome. Second, B[a]P exposure also caused biomacromolecules damage. The results demonstrated that mitochondria and microsome were more vulnerable to lipid peroxidation than cell membrane and nucleus. Taken together, the present study fills some of the gaps in our knowledge of the bioaccumulation and detoxification mechanisms of C. farreri exposed to B[a]P in subcellular fractions and deeply explores the transportation and the main metabolic and damage sites of polycyclic aromatic hydrocarbons (PAHs) in cells, which helped us to comprehensively understand the toxic mechanism of PAHs on bivalves. Environ Toxicol Chem 2022;41:2353–2364. © 2022 SETAC

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“…One detoxification route of NPs from biological systems is by elimination via conjugation to glutathione (GSH) catalyzed by the glutathione‐S‐transferases (GSTs; Arukwe et al, 2000; Sturve et al, 2006). GST is a well‐known biomarker of exposure to stress (Förlin et al, 1995) and has been analyzed inmammals, birds, molluscs, and fish of contaminated areasand in laboratory bioassays (Pérez‐López et al, 2002; Berglund et al, 2007). The effectiveness of GST activity as a biomarker of stress exposure has also been shown in nereidid worms, such as in Laeonereis acuta (Geracitano et al, 2002); Nereis diversicolor (Moreira et al, 2006; Dorou et al, 2007), and Nereis succinea (Rhee et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Glutathione‐S‐transferase in Nereis succinea (Polychaeta) and its induction by xeno‐estrogen

Ayoola

García‐Alonso

Hardege

2011

Environmental Toxicology

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The need to replace or at least to reduce the use of vertebrates in toxicity tests is a timely major concern in research and industry but to date, efforts made to minimize their use are still far from complete. Increasing demands for toxicity tests put considerable pressures upon the development of future fast and efficient test methods using invertebrates. In fact, to date, few studies provide links between biochemical and cellular effects of xeno-estrogens in aquatic invertebrates. Glutathione-S-transferase (GST) activity, as a biomarker of stress exposure, was measured in the population of clamworms (Nereis succinea) from Cardiff Bay. In addition, we examined the effect of single exposure to nonylphenol (NP) on this enzymatic activity. Field study results showed a relationship between the worm's size, reproductive status, and GST activity from the field population. In addition, we show a significant increase in the GST activity at 100 μg/L NP with sex-specific responses. The xeno-estrogens, which could affect reproduction of nereid by interfering in normal endocrinological pathways, are eliminated through GST by conjugation with glutathione. This work shows for the first time that GST activity depends on sex and stage of the clamworms and also that the xeno-estrogen NP induces its activity. This study supports the use of this species as a bioindicator of aquatic pollution and lays the foundation to causally link toxic exposure with reproductive output.

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Induction of cytosolic Glutathione S-transferases from Atlantic eel (Anguilla anguilla) after intraperitoneal treatment with polychlorinated biphenyls

Cited by 17 publications

References 18 publications

Disk Abalone, Haliotis discus discus, CuZn–Superoxide Dismutase cDNA and its Transcriptional Induction by Aroclor 1254

Disk Abalone, Haliotis discus discus, CuZn–Superoxide Dismutase cDNA and its Transcriptional Induction by Aroclor 1254

Bioaccumulation, Detoxification, and Biological Macromolecular Damage of Benzo[a]pyrene in Exposure in Tissues and Subcellular Fractions of Scallop Chlamys farreri

Glutathione‐S‐transferase in Nereis succinea (Polychaeta) and its induction by xeno‐estrogen

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