Induction of Decidualization in Rats by the Intrauterine Infusion of Prostaglandins1

Kennedy, T. G.; Lukash, L.

doi:10.1095/biolreprod27.1.253

Cited by 91 publications

(60 citation statements)

References 30 publications

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“…We also found that the size of the implantation sites and decidualization were severely affected by nimesulide, although nimesulide had no effect on the number of implantation sites (J. Cong et al, unpublished data). When infused into the lumen of sensitized rat uteri in which endogenous PG synthesis is inhibited, PGE2 is able to restore the endometrial vascular permeability response and decidualization (Kennedy & Lukash 1982, Keys & Kennedy 1990, Hamilton & Kennedy 1994. In steroid-injected ovariectomized rats, following unilateral intrauterine instillation of sesame oil, administration of indomethacin inhibited the onset of the decidual cell reaction and had deleterious effects on the luminal epithelium.…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown that prostaglandin E2 (PGE2) is able to restore the endometrial vascular permeability response and decidualization (Kennedy & Lukash 1982, Keys & Kennedy 1990, Hamilton & Kennedy 1994. Additionally, PGE2 plays a major role in the hatching of mouse blastocysts (Baskar et al 1981), is able to induce implantation of mouse blastocysts (Holmes & Gordashko 1980) and may also have immunomodulatory roles at the implantation site (Lala 1989).…”

Section: Introductionmentioning

confidence: 99%

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Differential expression and regulation of cylooxygenases, prostaglandin E synthases and prostacyclin synthase in rat uterus during the peri-implantation period

Cong¹,

Diao²,

Zhao³

et al. 2006

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It has been shown that both prostaglandin I2 (PGI2) and PGE2 are essential for mouse implantation, whereas only PGE2 is required for hamster implantation. To date, the expression and regulation of cyclooxygenase (COX) and prostaglandin E synthase (PGES), which are responsible for PGE2 production, have not been reported in the rat. The aim of this study was to examine the expression pattern and regulation of COX-1, COX-2, membrane-associated PGES-1 (mPGES-1), mPGES-2 and cytosolic PGES (cPGES) in rat uterus during early pregnancy and pseudopregnancy, and under delayed implantation. At implantation site on day 6 of pregnancy, COX-1 immunostaining was highly visible in the luminal epithelium, and COX-2 immunostaining was clearly observed in the subluminal stroma. Both mPGES-1 mRNA and protein were only observed in the subluminal stroma surrounding the implanting blastocyst at the implantation site on day 6 of pregancy , but were not seen in the inter-implantation site on day 6 of pregnancy and on day 6 of pseudopregnancy. Our data suggest that the presence of an active blastocyst is required for mPGES-1 expression at the implantation site. When pregnant rats on day 5 were treated with nimesulide for 24 h, mPGES-1 protein expression was completely inhibited. cPGES immunostaining was clearly observed in the luminal epithelium and subluminal stromal cells immediately surrounding the implanting blastocyst on day 6 of pregnancy. mPGES-2 immunostaining was clearly seen in the luminal epithelium at the implantation site. Additionally, immunostaining for prostaglandin I synthase (PGIS) was also strongly detected at the implantation site. In conclusion, our results indicate that PGE2 and PGI2 should have a very important role in rat implantation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Differential expression and regulation of cylooxygenases, prostaglandin E synthases and prostacyclin synthase in rat uterus during the peri-implantation period

Cong¹,

Diao²,

Zhao³

et al. 2006

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“…PGE 2 was infused in the same vehicle as the compounds to which it was being compared. Preparation of the pumps and the technique for inserting them have been described previously (Kennedy & Lukash 1982). In summary, one uterine horn of each animal was infused from the uterotubal end towards the cervical end.…”

Section: Intrauterine Infusionsmentioning

confidence: 99%

Expression patterns and role of prostaglandin-endoperoxide synthases, prostaglandin E synthases, prostacyclin synthase, prostacyclin receptor, peroxisome proliferator-activated receptor delta and retinoid x receptor alpha in rat endometrium during artificially-induced decidualization

Gillio-Meina¹,

Phang²,

Mather³

et al. 2009

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To determine if changes in endometrial expression of the enzymes and receptors involved in prostaglandin (PG) synthesis and action might provide insights into the PGs involved in the initiation of decidualization, ovariectomized steroid-treated rats at the equivalent of day 5 of pseudopregnancy were given a deciduogenic stimulus and killed at various times up to 32 h thereafter. The expression of PG-endoperoxide synthases (PTGS1 and PTGS2), microsomal PGE synthases (PTGES and PTGES2), cytosolic PGE synthase (PTGES3), prostacyclin synthase (PTGIS), prostacyclin receptor, peroxisome proliferator-activated receptor d (PPARD) and retinoid x receptor a (RXRA) in endometrium was assessed by semiquantitative RT-PCR, western blot analyses and immunohistochemistry. In addition, to determine which PG is involved in mediating decidualization, we compared the ability of PGE 2 , stable analogues of PGI 2 , L165041 (an agonist of PPARD), and docasahexanoic acid (an agonist of RXRA) to increase endometrial vascular permeability (EVP, an early event in decidualization), and decidualization when infused into the uterine horns of rats sensitized for the decidual cell reaction (DCR). EVP was assessed by uterine concentrations of Evans blue 10 h after initiation of infusions. DCR was assessed by the uterine mass 5 days after the initiation of the infusions. Because enzymes associated with the synthesis of PGE 2 , including PTGS2, are up-regulated in response to a deciduogenic stimulus and because PGE 2 was more effective than the PGI 2 analogues and PPARD and RXRA agonists in increasing EVP and inducing decidualization, we suggest that PGE 2 is most likely the PG involved in the initiation of decidualization in the rat.

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“…Arachidonic acid, but not oleic, palmitic or cu-dihomo-y-linolenic acids, applied to a suitably primed uterus also induces decidualization, an action prevented by indomethacin (Sananes et al, 1981). In addition, the intraluminal administration of sesame oil to a rat uterus sensitized for decidualization increases the endometrial concentrations of PGE and PGF within 5 min (Kennedy & Lukash, 1982). All these studies suggest that, at the time of implantation in the rat, the initial increase in capillary permeability is due to an increase solely in PGE-2 production within the endometrium, while the subsequent decidualization of the endometrium may be due to the increased local production of one or more PGs of the 2-series (including TXA-2).…”

Section: Introductionmentioning

confidence: 99%

“…Decidualization induced by artificial stimuli is also inhibited or reduced by indomethacin, while PGE-2, PGF-2oc, PGI-2 and 9,ll-epoxymethano-PGH-2 (a thromboxane A-2 receptor agonist) each induce decidualization of the suitably primed rat uterus (Tobert, 1976;Sananes, Baulieu & Le Goascogne, 1976Kennedy & Lukash, 1982;Miller & Morchoe, 1982a, b). Arachidonic acid, but not oleic, palmitic or cu-dihomo-y-linolenic acids, applied to a suitably primed uterus also induces decidualization, an action prevented by indomethacin (Sananes et al, 1981).…”

Section: Introductionmentioning

confidence: 99%

Effects of age and steroid treatment on prostaglandin production by the rat uterus in relation to implantation

Brown¹,

Gosden²,

Poyser³

1984

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Induction of Decidualization in Rats by the Intrauterine Infusion of Prostaglandins1

Cited by 91 publications

References 30 publications

Differential expression and regulation of cylooxygenases, prostaglandin E synthases and prostacyclin synthase in rat uterus during the peri-implantation period

Differential expression and regulation of cylooxygenases, prostaglandin E synthases and prostacyclin synthase in rat uterus during the peri-implantation period

Expression patterns and role of prostaglandin-endoperoxide synthases, prostaglandin E synthases, prostacyclin synthase, prostacyclin receptor, peroxisome proliferator-activated receptor delta and retinoid x receptor alpha in rat endometrium during artificially-induced decidualization

Effects of age and steroid treatment on prostaglandin production by the rat uterus in relation to implantation

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