Induction of dendritic cell maturation by β-glucan isolated from Sparassis crispa

Kim, Hyungsook; Kim, Jee Youn; Ryu, Hwa Sun; Park, Hyuk-Gu; Kim, Yong Oock; Kang, Jong Soon; Kim, Hwan Mook; Hong, Jin Tae; Kim, Youngsoo; Han, Sang‐Bae

doi:10.1016/j.intimp.2010.07.012

Cited by 59 publications

(34 citation statements)

References 45 publications

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“…21,22 The expressions of human airway mucins are induced in response to a wide variety of stimuli through a process involving MAPKs or NF-κB activation. 5,13,[23][24][25] Therefore, this study also focused on whether the activation of MAPKs and NF-κB might be associated with the upregulation of MUC4 and MUC5B expression by β-glucan.…”

Section: Discussionmentioning

confidence: 99%

Effect of β‐glucan on MUC4 and MUC5B expression in human airway epithelial cells

Kim

Bae

Song

et al. 2015

Int Forum Allergy Rhinol

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Section: Discussionmentioning

confidence: 99%

Effect of β‐glucan on MUC4 and MUC5B expression in human airway epithelial cells

Kim

Bae

Song

et al. 2015

Int Forum Allergy Rhinol

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“…The amount of incorporated [ 3 H]-thymidine was measured using a Microbeta scintillation counter (Wallac, Turku, Finland) (Kim et al 2010). …”

Section: Proliferation Assaymentioning

confidence: 99%

“…Lysates were prepared from total cells or nuclear fractions as previously described (Kim et al 2010). Detergentinsoluble materials were removed by centrifugation, and equal amounts of protein were fractionated by 10 % sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes.…”

Section: Western Blotsmentioning

confidence: 99%

Validation of cyclooxygenase-2 as a direct anti-inflammatory target of 4-O-methylhonokiol in zymosan-induced animal models

et al. 2014

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4-O-methylhonokiol (MH) is known to inhibit inflammation by partially understood mechanisms. Here, the anti-inflammatory mechanisms of MH were examined using enzymatic, cellular, and animal assays. In enzymatic assays, MH inhibited COX-2 activity with an IC50 of 0.062 μM, and also COX-1 with an IC50 of 2.4 μM. In cellular assays, MH was immunotoxic above 10 μM. At non-toxic concentrations (below 3 μM), MH strongly inhibited COX-2-mediated prostaglandin production with an IC50 of 0.1 μM, whereas did not or slightly affect other functions of B cells, T cells, dendritic cells, and macrophages. In an animal model, MH inhibited the increase in footpad thickness and popliteal lymph node weight in zymosan-injected mice. When analyzed the draining pLNs of zymosan-injected mice on day 5, MH inhibited the overall inflammatory responses. However, MH inhibited cyclooxygenase (COX)-2-mediated prostaglandin production without affecting tumor necrosis factor-α production in inflamed tissues within 6 h after zymosan injection. In summary, our data suggest that COX-2 may be a direct anti-inflammatory target of MH in vitro and in vivo.

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“…Recently, several studies have reported DC interactions with β-glucan. β-Glucan isolated from Umbilicaria esculenta induced expression of the DC maturation markers CD80, CD86, and MHC class I and II (13). This compound was also reported to increase production of the pro-inflammation cytokines IL-12, IL-6, and IL-8 (13).…”

Section: Maturation Of Dendritic Cells Via Cell Surface Markersmentioning

confidence: 92%

“…β-Glucan isolated from Umbilicaria esculenta induced expression of the DC maturation markers CD80, CD86, and MHC class I and II (13). This compound was also reported to increase production of the pro-inflammation cytokines IL-12, IL-6, and IL-8 (13). Moreover, β-glucan isolated from Sparassis crispa was demonstrated to be a strong inducer of DC maturation via the NF-κB and MAPK signaling pathways and proliferation of T cells for expression of a Th1 pattern cytokine response (11).…”

Section: Maturation Of Dendritic Cells Via Cell Surface Markersmentioning

confidence: 99%

β-(1,3)-glucan isolated from Agrobacterium species induces maturation of bone marrow-derived dendritic cells and drives Th1 immune responses

Byun

Sung

Park

et al. 2015

Food Sci Biotechnol

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The β-(1,3)-glucan originally isolated from Agrobacterium species was investigated for prompting maturation of dendritic cells (DCs) and driving Th1 immune responses. Bone-marrow derived DCs separated from mice were analyzed for augmentation of cell surface molecule (CD80, CD86, and major histo-compatability complex (MHC) class I/II) expression and pro-inflammatory cytokine production (tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6). β-Glucan functionally induced DCs activation via augmentation of CD80, CD86, and MHC class I/II expression and cytokine production (TNF-α, IL-1β, and IL-6). β-Glucan induced secretion of IL-12p70, but not IL-10. Both mitogen-activated protein kinases and nuclear factor (NF)-κB signaling mediated production of pro-inflammatory cytokine induced by β-glucan. β-Glucan-treated DCs exhibited accelerated proliferation of murine splenocytes with increased levels of interferon (IFN)-γ. β-Glucan regulates innate and adaptive immunity via DCs activation and Th1 polarization of immune responses.

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Induction of dendritic cell maturation by β-glucan isolated from Sparassis crispa

Cited by 59 publications

References 45 publications

Effect of β‐glucan on MUC4 and MUC5B expression in human airway epithelial cells

Effect of β‐glucan on MUC4 and MUC5B expression in human airway epithelial cells

Validation of cyclooxygenase-2 as a direct anti-inflammatory target of 4-O-methylhonokiol in zymosan-induced animal models

β-(1,3)-glucan isolated from Agrobacterium species induces maturation of bone marrow-derived dendritic cells and drives Th1 immune responses

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