Induction of developmental hematopoiesis mediated by transcription factors and the hematopoietic microenvironment

Daniel, Michael G.; Rapp, Katrina; Schaniel, Christoph; Moore, Kateri

doi:10.1111/nyas.14246

Cited by 11 publications

(9 citation statements)

References 141 publications

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“…To clarify this controversial issue, delicate in vivo fate mapping studies are required. In parallel, although multiple signaling pathways including Hedgehog, Wnt, Vegf, Notch, and BMP are reported critical for HEC and cEC fate specification ( 18 – 21 ), it seems that these signaling pathways eventually converge on the ECs-autonomous requirement of Notch signaling to activate key downstream transcriptional factors, including Gata2, Runx1, and Gfi1 ( 22 – 24 ). Besides, these key factors are also regulated by the upstream factors, including Etv2 and Scl ( 25 – 27 ).…”

mentioning

confidence: 99%

Hemogenic and aortic endothelium arise from a common hemogenic angioblast precursor and are specified by the Etv2 dosage

Zhao

Feng

Tian

et al. 2022

Proc. Natl. Acad. Sci. U.S.A.

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Significance Hematopoietic stem cells (HSCs) are generated from specialized endothelial cells, called hemogenic endothelial cells (HECs). It has been debated whether HECs and non–HSC-forming conventional endothelial cells (cECs) arise from a common precursor or represent distinct lineages. Moreover, the molecular basis underlying their distinct fate determination is poorly understood. We use photoconvertible labeling, time-lapse imaging, and single-cell RNA-sequencing analysis to trace the lineage of HECs. We discovered that HECs and cECs arise from a common hemogenic angioblast precursor, and their distinct fate is determined by high or low dosage of Etv2, respectively. Our results illuminate the lineage origin and a mechanism on the fate determination of HECs, which may enhance the understanding on the ontogeny of HECs in vertebrates.

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mentioning

confidence: 99%

Hemogenic and aortic endothelium arise from a common hemogenic angioblast precursor and are specified by the Etv2 dosage

Zhao

Feng

Tian

et al. 2022

Proc. Natl. Acad. Sci. U.S.A.

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“…Haematopoietic cells can emerge from a common mesodermal progenitor or VE-Cadherin + ECs. They appear in the yolk sac and the dorsal aorta, but with the onset of circulation, they are able to migrate to and interact with multiple niches [77,78].…”

Section: Discussionmentioning

confidence: 99%

Haematopoietic stem cell reprogramming and the hope for a universal blood product

2019

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Edited by Catherine RobinHaematopoietic stem cells (HSCs) are the only adult stem cells with a demonstrated clinical use, even though a tractable method to maintain and expand human HSCs in vitro has not yet been found. Owing to the introduction of transplantation strategies for the treatment of haematological malignancies and, more recently, the promise of gene therapy, the need to improve the generation, manipulation and scalability of autologous or allogeneic HSCs has risen steeply over the past decade. In that context, reprogramming strategies based on the expression of exogenous transcription factors have emerged as a means to produce functional HSCs in vitro. These approaches largely stem from the assumption that key master transcription factors direct the expression of downstream target genes thereby triggering haematopoiesis. Both somatic and pluripotent cells have been used to this end, yielding variable results in terms of haematopoietic phenotype and functionality. Here, we present an overview of the haematopoietic reprogramming methods reported to date, provide the appropriate historical context and offer some critical insight about where the field stands at present.

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“…red blood cells, platelets, megakaryocytes, Tcells) from pluripotent stem cells or somatic cells, through reprogramming or transgene free protocols, is achievable (159). Reprogramming by (transient) expression of transcription factors is also a very promising strategy to generate HSC-like cells in vitro since a decade (11,(160)(161)(162)(163)(164)(165)(166). However, these HSC-like cells are produced at a very low yield and remain limited in their capacities to self-renew and/or to replenish all blood lineages, which is an absolute requirement for therapeutic use.…”

Section: Recapitulating the Endogenous Hsc Niche In 3d-culture Systems To Produce Bona Fide Hscs And Other Blood Forming Cellsmentioning

confidence: 99%

Recent Advances in Developmental Hematopoiesis: Diving Deeper With New Technologies

2021

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The journey of a hematopoietic stem cell (HSC) involves the passage through successive anatomical sites where HSCs are in direct contact with their surrounding microenvironment, also known as niche. These spatial and temporal cellular interactions throughout development are required for the acquisition of stem cell properties, and for maintaining the HSC pool through balancing self-renewal, quiescence and lineage commitment. Understanding the context and consequences of these interactions will be imperative for our understanding of HSC biology and will lead to the improvement of in vitro production of HSCs for clinical purposes. The aorta-gonad-mesonephros (AGM) region is in this light of particular interest since this is the cradle of HSC emergence during the embryonic development of all vertebrate species. In this review, we will focus on the developmental origin of HSCs and will discuss the novel technological approaches and recent progress made to identify the cellular composition of the HSC supportive niche and the underlying molecular events occurring in the AGM region.

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Induction of developmental hematopoiesis mediated by transcription factors and the hematopoietic microenvironment

Cited by 11 publications

References 141 publications

Hemogenic and aortic endothelium arise from a common hemogenic angioblast precursor and are specified by the Etv2 dosage

Hemogenic and aortic endothelium arise from a common hemogenic angioblast precursor and are specified by the Etv2 dosage

Haematopoietic stem cell reprogramming and the hope for a universal blood product

Recent Advances in Developmental Hematopoiesis: Diving Deeper With New Technologies

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