Induction of differentiation in psoriatic keratinocytes by propylthiouracil and fructose

Arul, Santhosh; Dayalan, Haripriya; Jegadeesan, Muhilan; Damodharan, Prabhavathy

doi:10.1016/j.bbacli.2016.06.002

Cited by 13 publications

(13 citation statements)

References 25 publications

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“…The expression of 9 type I and 9 type II keratins were detected in the volar forearm skin (Table I). These proteins have various functions, including stabilizing cytoskeletal elements, modulating cellular metabolism, regulating cellular differentiation and proliferation, as well as mediating inflammatory pathways (5)(6)(7)25). The keratin pair K5/K14 is expressed in the basal layer, and mutations in K5 or K14 cause 75% of the basal subtypes of epidermolysis bullosa simplex (EBS) (14,26,27) and a rare keratin disease known as dowling-degos disease, as well as its variant named Galli-Galli disease (14,28).…”

Section: Discussionmentioning

confidence: 99%

Non‑invasive proteome‑wide quantification of skin barrier‑related proteins using label‑free LC‑MS/MS analysis

et al. 2020

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a number of epidermal proteins are closely related to skin barrier function, the abnormalities of which can lead to specific skin diseases. These proteins must be quantified to further investigate the changes in the skin barrier between healthy and disease states. However, the non-invasive and proteome-wide quantification of skin proteins without any labelling steps remains a challenge. In this study, 3M medical adhesive tapes were used to obtain skin samples from volunteers. Proteins were extracted from fresh skin samples and digested with trypsin. Each tryptic peptide was analysed in three replicates using liquid chromatography with tandem mass spectrometry analysis and label-free quantification. The data were searched against the Human Universal Protein Resource (UniProt) to match with known proteins. Using this method, 1,157 skin proteins recorded in the uniProt were quantified. A total of 50 identical proteins were identified in the three replicate analyses of all samples with no significant differences in abundance. The results provided an objective metric for further study of skin ageing and various skin diseases. Specifically, the non-invasive proteome-wide method used in this study can be applied to future studies of skin diseases related to barrier destruction by monitoring the changes in the levels of epidermal proteins.

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Section: Discussionmentioning

confidence: 99%

Non‑invasive proteome‑wide quantification of skin barrier‑related proteins using label‑free LC‑MS/MS analysis

et al. 2020

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“…Psoriasis is an inflammatory skin disease and usually identified by erythematous, raised, scaly skin lesions. Uncontrolled proliferation and poor differentiation of keratinocytes is characterized in lesional psoriatic skin . There are three different processes of cellular alteration in psoriatic skin: abnormal differentiation of keratinocytes, hyperproliferation of keratinocytes and infiltration of immune cells into the dermis and epidermis …”

Section: Discussionmentioning

confidence: 99%

“…Uncontrolled proliferation and poor differentiation of keratinocytes is characterized in lesional psoriatic skin. [17] There are three different processes of cellular alteration in psoriatic skin: abnormal differentiation of keratinocytes, hyperproliferation of keratinocytes and infiltration of immune cells into the dermis and epidermis. [19] Multiple mechanisms have been elucidated to regulate proliferation and differentiation of keratinocytes in psoriasis.…”

Section: Discussionmentioning

confidence: 99%

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MiR‐744‐3p regulates keratinocyte proliferation and differentiation via targeting KLLN in psoriasis

Wang

Zong

et al. 2019

Experimental Dermatology

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Psoriasis is a chronic inflammatory disease, and microRNAs have been reported to regulate the pathogenesis of psoriasis. Up‐regulated miR‐744‐3p level was identified to associate with psoriasis while the precise functions of miR‐744‐3p in psoriasis were not well‐elucidated. We first confirmed the up‐regulation of miR‐744‐3p in psoriasis by measuring its expression level in psoriatic samples. We explored the roles of miR‐744‐3p on keratinocytes proliferation and differentiation. We searched the targets of miR‐744‐3p and evaluated the roles of one target, KLLN on keratinocytes proliferation and differentiation. We confirmed the up‐regulation of miR‐744‐3p in psoriatic samples. MiR‐744‐3p promoted keratinocytes proliferation while inhibited differentiation. MiR‐744‐3p targeted KLLN and overexpression of miR‐744‐3p resulted in decreased expression of KLLN. Overexpression of KLLN prevented the effects of miR‐744‐3p on keratinocytes proliferation and differentiation. MiR‐744‐3p regulated the proliferation and differentiation of keratinocytes through targeting KLLN in psoriasis.

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“…Its concentration in the extracellular space varies in the individual layers of skin, and it is around four times higher in the upper parts of the epidermis (between 1.2 and 1.8 mM) compared to the basal layer (from 0.05 to 1.0 mM). The calcium gradient is sufficient to induce cell proliferation, but further transformation requires higher concentrations both in the extracellular space and in the cytosol [80][81][82]. The presence of the cell nuclei together with the increased proliferation rate of epidermal cells occurs in the course of psoriasis.…”

Section: Keratinocytes' Differentiationmentioning

confidence: 99%

Unbalanced Sphingolipid Metabolism and Its Implications for the Pathogenesis of Psoriasis

Bocheńska

Gabig-Cimińska

2020

Molecules

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Sphingolipids (SLs), which have structural and biological responsibilities in the human epidermis, are importantly involved in the maintenance of the skin barrier and regulate cellular processes, such as the proliferation, differentiation and apoptosis of keratinocytes (KCs). As many dermatologic diseases, including psoriasis (PsO), intricately characterized by perturbations in these cellular processes, are associated with altered composition and unbalanced metabolism of epidermal SLs, more education to precisely determine the role of SLs, especially in the pathogenesis of skin disorders, is needed. PsO is caused by a complex interplay between skin barrier disruption, immune dysregulation, host genetics and environmental triggers. The contribution of particular cellular compartments and organelles in SL metabolism, a process related to dysfunction of lysosomes in PsO, seems to have a significant impact on lysosomal signalling linked to a modulation of the immune-mediated inflammation accompanying this dermatosis and is not fully understood. It is also worth noting that a prominent skin disorder, such as PsO, has diminished levels of the main epidermal SL ceramide (Cer), reflecting altered SL metabolism, that may contribute not only to pathogenesis but also to disease severity and/or progression. This review provides a brief synopsis of the implications of SLs in PsO, aims to elucidate the roles of these molecules in complex cellular processes deregulated in diseased skin tissue and highlights the need for increased research in the field. The significance of SLs as structural and signalling molecules and their actions in inflammation, in which these components are factors responsible for vascular endothelium abnormalities in the development of PsO, are discussed.

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Induction of differentiation in psoriatic keratinocytes by propylthiouracil and fructose

Cited by 13 publications

References 25 publications

Non‑invasive proteome‑wide quantification of skin barrier‑related proteins using label‑free LC‑MS/MS analysis

Non‑invasive proteome‑wide quantification of skin barrier‑related proteins using label‑free LC‑MS/MS analysis

MiR‐744‐3p regulates keratinocyte proliferation and differentiation via targeting KLLN in psoriasis

Unbalanced Sphingolipid Metabolism and Its Implications for the Pathogenesis of Psoriasis

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