Induction of Dog Sperm Capacitation by Oviductal Fluid.

Kawakami, Eiichi; Hori, Tatsuya; Tsutsui, Toshihiko

doi:10.1292/jvms.60.197

Cited by 22 publications

(17 citation statements)

References 39 publications

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“…In addition, the sperm reservoir controls the progression of suitable spermatozoa, in very restricted numbers, to the site of fertilization of the newly ovulated oocytes [32,34,38]. Moreover, various GAGs have been found to be able to maintain sperm motility and induce capacitation in vitro in bovine [39] and other species [40][41][42].…”

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confidence: 99%

Detection of the Hyaluronan Receptor CD44 in the Bovine Oviductal Epithelium

et al. 2005

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Abstract. Hyaluronan is involved in fundamental reproductive events such as sperm storage in the female reproductive tract, fertilization, and early embryo development, these functions are presumably mediated by its major cell surface receptor, CD44. The present study was conducted to investigate the presence and localization of CD44 in the bovine oviductal epithelium, using immunohistochemical and Western blot methods on tissue sections and epithelial cell extracts collected from the uterotubal junction (UTJ), isthmus, and ampulla of animals in the oestrus or luteal phase of the oestrous cycle. While positive immunolabelling for CD44 was found on the ad-luminal surface and supra-nuclear region of epithelial cells in all tubal segments investigated, in the UTJ, there were epithelial cells in which the entire cytoplasm positively stained. We found no differences in terms of CD44-positive staining between the different stages of the oestrous cycle. Presence of CD44 was detected by Western blotting in the tubal epithelium as a single band at 200 kDa. Although it appeared in all tubal segments, the expression of CD44 protein was more accentuated in the sperm reservoir (UTJ) than in the other segments. This is the first time CD44 has been detected in the epithelium of the tubal sperm reservoir in cattle, suggesting a pathway for the action of hyaluronan in this segment.

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confidence: 99%

Detection of the Hyaluronan Receptor CD44 in the Bovine Oviductal Epithelium

et al. 2005

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“…The percentage of HA-sperm correlated with the percentage of PHA-E-unlabeled sperm. These results suggest that HA-sperm move- ment-inducing substances are present in the oviductal fluid of estrous bitches, the same as in other species [12,15,22], and that loss of PHA-E lectin-binding glycoprotein on the surface of canine sperm is associated with the start of sperm capacitation. The authors [14] previously reported that glycosaminoglycans, a class of mucopolysaccharide, in the oviductal fluid of the estrous bitches induce HA-movement of canine sperm, the same as in the cow [1,8].…”

Section: Discussionmentioning

confidence: 58%

Disappearance of the PHA-E Lectin Binding Site on the Surface of Ejaculated Sperm and Sperm Capacitation in the Dog

Kawakami

Sato

Hirano

et al. 2004

The Journal of Veterinary Medical Science

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ABSTRACT. Ejaculated semen and cross sections of the cauda epididymides collected from 3 normal dogs were smeared or stamped on glass slides, and the sperm on the slides were stained with 7 different FITC-lectins (Con A, DBA, GS-1, PHA-E, PSA, UEA-1, WGA) to examine the relation between sperm-binding glycoprotein derived from the canine prostate and sperm capacitation. The only lectin that stained the ejaculated sperm but not the cauda epididymal sperm was PHA-E. The sperm ejaculated by 5 other dogs were incub ated for 4 hr in fluid flushed from the uterine horns or oviducts of estrous bitches, and then the percentages of actively motile sperm and hyperactivated sperm (HA-sperm) were determined. The percentages of PHA-E-labeled sperm and sperm labeled with fluoresceinated Ca indicator to assess the influx of Ca into the sperm were also evaluated. The mean percentages of actively motile sperm, HA-sperm, and Ca-labeled sperm after 4 hr of incubation in the uterine flush fluid and oviductal flush fluid were significantly higher tha n in control medium (P<0.05, 0.01), but the mean percentages of PHA-E-labeled sperm were lower (both P<0.01). The percentages of PHA-E-unlabeled sperm correlated with the percentages of both HA-sperm and Ca-labeled sperm (r 2 =0.787 and 0.812, respectively). The results indicate that loss of the glycoprotein secreted by the canine prostate on the sperm surface induces the influx of Ca into the sperm, and then hyperactivation of the sperm. KEY WORDS: canine, capacitation, lectin, prostate, sperm.J. Vet. Med. Sci. 66 (5): [495][496][497][498][499][500] 2004 It has been reported that the surface of the plasma membrane of mammalian sperm becomes coated with various glycoproteins secreted by the epididymal epithelium during sperm transit through the epididymis [9,11], and some of the glycoproteins on the sperm surface are thought to induce sperm maturation in the epididymal duct [10,17,29]. It is known that the glycoproteins produced by the prostates and seminal vesicles are contained in the semen [2,5,24] and that some of them act as decapacitation factors [2,24]. The decapacitation factors on the surface of the sperm are lost as a result of the action of proteolytic enzymes in the uterus and oviduct [5,6,19], and sperm capacitation is then promoted by some components of oviductal fluid, e.g., glycosaminoglycans [11,15,22]. Sperm capacitation is induced by an influx of Ca into the cytoplasm of the sperm [23,25,27]. Techniques utilizing many different fluorescein-isothiocyanate (FITC)-labeled lectins have revealed the characteristics of the glycoproteins coating the surface of the sperm in many species [9,[16][17][18]29], but there have been few reports on the relation between sperm-binding glycoproteins and sperm capacitation in the dog [3,7]. In the present study, canine epididymal sperm and ejaculated sperm were stained with 7 different FITC-lectins to examine for the presence of sperm-binding glycoproteins derived from canine prostate in the seminal fluid and to assess the relation bet...

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“…THE ZP-binding assay was performed by the method described previously [11]. Briefly, after the oocytes were thawed at room temperature, three oocytes were placed in each of three 100-µl droplets of sperm suspension that had been incubated for 24 hr under paraffin oil in 35-mm plastic tissue culture dishes.…”

Section: Collection Of Testicular and Epididymal Spermmentioning

confidence: 99%

Lectin-Binding Characteristics and Capacitation of Canine Epididymal Spermatozoa

Kawakami

Morita

Hori

et al. 2002

The Journal of Veterinary Medical Science

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ABSTRACT. Cross sections of the testes and the caput, corpus and cauda epididymides removed from 12 dogs were stamped on glass slides, and the sperm on the slides were stained with 6 different FITC-lectins (Con A, DBA, PNA, PSA, SBA, and WGA) to examine the characteristics of the surface glycoproteins (GPs) on canine epididymal sperm. The corpus epididymal sperm were washed three times by centrifugation, and their lectin-binding characteristics were investigated. The washed sperm from the corpus and cauda epididymides were incubated for 24 hr, and the fertilizing capacity of the sperm was evaluated by calculating the percentages of actively motile sperm (%MO), hyperactivated sperm (%HA), and acrosome-reacted sperm (%AR), and the number of canine zona-pellucida (ZP)-binding sperm. The testicular sperm did not stain with SBA lectin, but the SBA lectin fluorescence was observed on the surface of the enitire heads of the caput epididymal sperm. Although all of the entire heads or acrosomal regions of the corpus epididymal sperm stained with all 6 FITC-lectins, the heads and acrosomal regions of the cauda epididymal sperm did not stain with DBA or SBA lectins. Washing the sperm from the corpus epididymis resulted in loss of the fluorescence of the FITC-DBA and -SBA lectins. The mean %MO, %HA, %AR, and ZP-binding number of the cauda epididymal sperm after 24 hr of incubation were higher than the values for the corpus epididymal sperm. All of the mean values for the washed sperm from the corpus and cauda epididymides were higher than the values for the unwashed sperm from the corpus and cauda, and with the exception of %AR, the values from the washed sperm from the corpus epididymis were significantly higher (P<0.05, 0.01). The results indicate that DBA-and SBA-lectin-binding GPs on the surface of canine epididymal sperm are associated with the fertilizing capacity and may be decapacitation factors. KEY WORDS: canine, capacitation, epididymis, lectin, sperm.J. Vet. Med. Sci. 64 (7): [543][544][545][546][547][548][549] 2002 It is well-known that the mammalian sperm plasma membrane surface is coated with various glycoproteins (GPs) [8,9,20,21,25]. Although some GPs are already present on the surface of immature sperm in the seminiferous tubules of the testis [18,19], the surface of the sperm becomes covered with a wide variety of GPs secreted by the epididymal epithelium during sperm transit through the epididymis [5,6,17,23]. Sperm surface GPs are thought to induce sperm maturation and fertilizing capacity [10,20] in the epididymal duct, and techniques utilizing fluorescein-isothiocyanate (FITC)-labeled lectins have revealed that the sperm surface GPs are biochemically modified during sperm transit through the epididymis and that there are species-dependent differences in sperm surface GPs [1,5,6,17,20,23]. Some of the GPs on the surface of epididymal sperm have been suspected of acting as decapacitation factors (DFs) [16,21], and while sperm fertilizing capacity is induced by removing DFs from the sperm surface [7,1...

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Induction of Dog Sperm Capacitation by Oviductal Fluid.

Cited by 22 publications

References 39 publications

Detection of the Hyaluronan Receptor CD44 in the Bovine Oviductal Epithelium

Detection of the Hyaluronan Receptor CD44 in the Bovine Oviductal Epithelium

Disappearance of the PHA-E Lectin Binding Site on the Surface of Ejaculated Sperm and Sperm Capacitation in the Dog

Lectin-Binding Characteristics and Capacitation of Canine Epididymal Spermatozoa

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