Induction of EROD Activity and Genotoxic Effects by Polycyclic Aromatic Hydrocarbons and Resin Acids on the Juvenile Eel (Anguilla anguillaL.)

Pacheco, Mário; Santos, M.A.

doi:10.1006/eesa.1997.1585

Cited by 108 publications

(79 citation statements)

References 43 publications

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“…The A. anguilla genotoxic response to the contaminants tested confirmed previous investigations where BaP (Hawkins et al, 1990;Pacheco and Santos, 1997), DHAA (Pacheco and Santos, 1999), and BKPME (Das and Nanda, 1986;Al-Sabti and Ha¨rdig, 1990;Pacheco and Santos, 1999) were identified as strong fish mutagens. However, the BaP results reveal a clear doserelated disruption of ENA expression demonstrated by the inability of the highest concentration to induce a significant ENA increase as well as by the inverse doseresponse relationship observed at 30 days exposure.…”

Section: Ena Responsesupporting

confidence: 87%

“…Microsomes were obtained according to the methods of Lange et al (1993) and Monod and Vindimian (1991) as adapted by Pacheco and Santos (1997). Total EROD activity was measured as described by Burke and Mayer (1974), and the results were expressed as pmol/min/mg of microsomal protein.…”

Section: Erod Assaymentioning

confidence: 99%

“…Several fish studies have reported BaP (Wolkers et al, 1996;Pacheco and Santos, 1997), DHAA and BKPME (Martel et al, 1994;Pacheco and Santos, 1999) as EROD inducers. Considering these previous investigations, the A. anguilla biotransformation response observed in the current research work seems unexpected, since neither BaP nor BKPME exhibited considerable total EROD induction.…”

Section: Erod Responsementioning

confidence: 99%

“…BaP, a PAH commonly found in contaminated environments, is recognized as a biotransformation inducer (Wolkers et al, 1996;Pacheco and Santos, 1998) with strong genotoxic and carcinogenic potential in fish (Hendricks et al, 1985;Hawkins et al, 1990;Pacheco and Santos, 1997). Effluents from the wood processing industry are one of the most significant sources of aquatic pollution worldwide.…”

Section: Introductionmentioning

confidence: 99%

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Biotransformation, genotoxic, and histopathological effects of environmental contaminants in European eel (Anguilla anguilla L.)

Pacheco

Santos

2002

Ecotoxicology and Environmental Safety

268

125

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A prolonged toxicity study was carried out in young European eel (Anguilla anguilla L.) to evaluate the effects of environmental contaminants, namely, two individual standard compounds, benzo [a]pyrene (BaP) and dehydroabietic acid (DHAA), and a complex mixture, bleached kraft pulp mill effluent (BKPME). Fish were exposed to BaP (0.22, 0.45, and 0.9 mM) and BKPME (3.12%, 6.25%, and 12.5% (v/v)) for 3, 7, and 30 days and to DHAA (0.07, 0.15, and 0.30 mM) for 3, 7, 30, 90, and 180 days. The biomarkers include biotransformation and genotoxicity indicators, such as total ethoxyresorufin O-deethylase (EROD) activity and frequency of erythrocytic nuclear abnormalities (ENAs), respectively. Hematological dynamics was assessed as frequency of immature erythrocytes (IEs). Histopathological examinations were carried out for the highest concentrations and for 30 days and longer exposures. Total EROD increases significantly only after 180 days of DHAA exposure. However, significant ENA induction was generally observed during exposure to all contaminants tested. Nevertheless, some of the ENA results suggest an altered genotoxic response, which may arise either from short-term exposures to the highest contaminant levels or long-term exposures to the lowest contaminant levels. IE frequency decreased significantly after 30 days of exposure to 0.45 mM BaP and 180 days of exposure to the entire DHAA concentration range. Increased density of pigmented macrophage aggregates in 30-day BaP-and BKPME-exposed fish as well as in 90-and 180-day DHAA-exposed fish confirmed histopathological liver alterations. Bile accumulation in hepatocytes after BaP treatment, cytoplasmic vacuolization and cell atrophy following DHAA exposure, as well as liver loss of parenchymal cells in BKPME-exposed fish, were also detected. Dispersed necrosis and focal inflammation were observed in the livers of all treated groups. Fish exposed to DHAA and BKPME showed skin and gill disruption as well as kidney Malpighian corpuscle alterations. All 30-day-treated groups revealed intense spleen hemosiderosis, indicating increased erythrophagia. This splenic effect may be strongly correlated with the observed disappearance of ENAs. Neoplastic lesions were not found. A multibiomarker strategy, which includes EROD, ENA, and IE assays as well as histopathological studies, contributed to a better understanding of the global toxic process. r

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Section: Ena Responsesupporting

confidence: 87%

Section: Erod Assaymentioning

confidence: 99%

Section: Erod Responsementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biotransformation, genotoxic, and histopathological effects of environmental contaminants in European eel (Anguilla anguilla L.)

Pacheco

Santos

2002

Ecotoxicology and Environmental Safety

268

125

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“…Micronuclei assay detects both clastogenic and aneugenic effects and therefore can detect the genotoxicity of a wide range of compounds (Heddle et al, 1991). Nuclear abnormalities, such as micronuclei and other nuclear malformations, are considered good indicators of cytotoxicity and genotoxicity, respectively (Hose et al, 1987;Metcalfe, 1988;Pacheco & Santos, 1996, 1997Sanchez-Galan et al, 1998;Ayllon & Garcia-Vazquez, 2000;Ayllon & Garcia-Vasquez, 2001, Kirschbaum, et al, 2009.…”

Section: Introductionmentioning

confidence: 99%

Water Toxicity and Cyto-genotoxicity Biomarkers in the Fish Oreochromis niloticus (Cichlidae)

Seriani¹,

Abessa²,

Kirschbaum³

et al. 2024

Ecotoxicol. Environ. Contam.

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Micronucleus (MN) and nuclear abnormalities (NA) tests were used as biomarkers in the fish Oreochromis niloticus from a polluted pond in winter, spring and summer. Samples of water column were taken aiming to evaluate physical-chemical parameters and acute toxicity to the microcrustacean Daphnia similis. The pond waters were toxic to D. similis in spring and winter, but this toxicity was not detected in summer. The pond waters presented low levels of dissolved oxygen in spring and summer; higher ammonia concentrations in winter, and high hardness and conductivity. Fish collected at the polluted pond exhibited higher rates of DNA damage, as indicated by increased rates of MN and NA in erythrocytes when compared to the negative control, which were associated to water toxicity and physical-chemical variable. Thus, this study shows that results of water toxicity tests and genotoxic biomarkers in fish may be associated and theses approaches may be used together for the water quality assessment.Keywords: fish, cyto-genotoxicity, pollution, ecotoxicology, water quality. Toxicidade da água e biomarcadores de citogenotoxicidade em Oreochromis niloticus (CiChlidae) ResumoNo presente estudo foi utilizado o teste do micronúcleo (MN) e de anomalias na morfologia dos núcleos de eritrócitos periféricos (NA) do peixe Oreochromis niloticus para avaliar o potencial cito-genotóxico de substâncias tóxicas encontradas na água durante inverno, primavera e verão em um lago poluído. Amostras de água foram coletadas para avaliar parâmetros físico-químicos e toxicidade aguda para o microcrustáceo Daphnia similis. Os resultados indicaram baixos teores de oxigênio dissolvido na água durante a primavera e no verão, elevados níveis de amônia no inverno, além de dureza e condutividade altas e toxicidade aguda na primavera e no inverno, não assim no verão. Os peixes também apresentaram danos cito-genotóxicos, em todas as coletas, e que foram associados com a toxicidade e os parâmetros físico-químicos da água. Nesse sentido o presente estudo mostra que os resultados de testes de toxicidade aguda e de biomarcadores de cito-genotóxicidade em peixes podem estar associados e podem ser utilizados de forma conjunta para na avaliação da qualidade das águas.Palavras-chave: peixes, cito-genotoxicidade, poluição, ecotoxicologia, qualidade de água.

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PAHs: An Ecotoxicological Perspective

Douben¹

2003

120

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Induction of EROD Activity and Genotoxic Effects by Polycyclic Aromatic Hydrocarbons and Resin Acids on the Juvenile Eel (Anguilla anguillaL.)

Cited by 108 publications

References 43 publications

Biotransformation, genotoxic, and histopathological effects of environmental contaminants in European eel (Anguilla anguilla L.)

Biotransformation, genotoxic, and histopathological effects of environmental contaminants in European eel (Anguilla anguilla L.)

Water Toxicity and Cyto-genotoxicity Biomarkers in the Fish Oreochromis niloticus (Cichlidae)

PAHs: An Ecotoxicological Perspective

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