Induction of esterase activity during the degradation of high concentrations of the contaminant di(2-ethylhexyl) phthalate by Fusarium culmorum under liquid fermentation conditions

González-Marquez, Ángel; Loera-Corral, Octavio; Viniegra‐González, Gustavo; Sánchez, Carmen

doi:10.1007/s13205-020-02476-y

Cited by 9 publications

(6 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These microorganisms may enhance pollutant degradation in phthalate-contaminated environments. Several investigations have revealed that fungal species such as Polyporus brumalis , Ganoderma lucidum , Trametes versicolor , Phlebia tremellosa , Neurospora sithopila , P. ostreatus , Phoma sp., Stropharia rugosoannulata , Ascocoryn e sp., Puccinia arenariae are phthalate-degrading organisms (Hwang et al, 2008 ; Kim et al, 2008 ; Lee et al, 2007 ; Kim & Song, 2009 ; Ahuactzin-Pérez et al., 2018a , 2018b ; Carstens et al, 2020 ; González-Márquez et al, 2019 ; González-Márquez et al, 2020 ; Hwang et al, 2012 ; Liao et al, 2012 ; Luo et al, 2012 ; Sánchez, 2021 ; Sánchez-Sánchez & Sánchez, 2019 ). In particular, fungi such as Fusarium species have been reported to degrade phthalates due to their esterase production.…”

Section: Introductionmentioning

confidence: 99%

Enhanced esterase activity during the degradation of dibutyl phthalate by Fusarium species in liquid fermentation

González-Marquez

Volke-Sepúlveda

Díaz

et al. 2021

Journal of Industrial Microbiology and Biotechnology

Self Cite

View full text Add to dashboard Cite

Dibutyl phthalate (DBP) is one of the most abundantly produced and used plasticizers and is incorporated into plastic to make it more flexible and malleable. DBP has been found to be an environmental contaminant and reported as an endocrine disruptor. Therefore, it is crucial to develop ecofriendly alternatives to eliminate phthalate pollution. In the present research, the growth of F. culmorum and F. oxysporum in the presence of DBP was studied in liquid fermentation. The esterase activity, specific growth rate, and growth and enzymatic yield parameters were determined in DBP-supplemented media (1500 or 2000 mg/L) and in control medium (lacking DBP). These results show that in general, for both Fusarium species, the highest esterase activities, specific growth rates, and yield parameters were observed in media supplemented with DBP. It was observed that 1500 and 2000 mg of DBP/L did not inhibit F. culmorum or F. oxysporum growth and that DBP induced esterase production in both fungi. These organisms have much to offer in the mitigation of environmental pollution caused by the endocrine disruptor DBP. This study reports, for the first time, esterase production during the degradation of high concentrations (i.e.1500 and 2000 mg/L) of DBP by F. culmorum F. oxysporum.

show abstract

Section: Introductionmentioning

confidence: 99%

Enhanced esterase activity during the degradation of dibutyl phthalate by Fusarium species in liquid fermentation

González-Marquez

Volke-Sepúlveda

Díaz

et al. 2021

Journal of Industrial Microbiology and Biotechnology

Self Cite

View full text Add to dashboard Cite

show abstract

“…The pH value in the cultures is an indicator of the substrate degradation process. Acid molecules were released into the media during the first 24 h of fermentation, decreasing the pH value, whereas amino compounds were formed after 32 h, which increased the pH value (González-Márquez et al 2020;González-Márquez and Sánchez 2022). Gonzalez-Márquez et al (2019a) studied the growth of the fungus F. culmorum using different apple cutin concentrations (0.2, 2, and 20 g/L) under submerged fermentation conditions and found that the pH of the cultures increased from the initial pH 6.5 to pH 7.5 after 168 h. The pH variation of the fungal cultures depends on the substrate composition and fermentation system.…”

Section: Time Of Fermentation (H)mentioning

confidence: 99%

“…The production of cutinases is regulated by microbial growth conditions, carbon source, and fermentation system (Liang and Zou 2023). The enzymatic activity of cutinases is induced by the presence of cutin and cutin monomers in the culture medium (Degani 2015;González-Márquez et al 2019a;González-Márquez and Sánchez 2022). Cutin is the main component of the plant cell wall.…”

Section: Introductionmentioning

confidence: 99%

Cutinolytic esterases are induced by growth of the fungus Trichoderma harzianum on glyceryl monostearate in solid-state fermentation

Conde-Ávila,

Loera-Corral,

Díaz

et al. 2023

BioRes

View full text Add to dashboard Cite

Cutinolytic esterase are enzymes utilized in a wide variety of industrial applications, and they are capable of degrading emerging environmental pollutants. Due to the application and importance of these enzymes, it is crucial to develop an efficient method for cutinase production using a cost-effective inductor and an efficient microbial production system. In this work, the growth and cutinolytic esterase production of Trichoderma harzianum were evaluated in glucose-yeast extract media containing different glyceryl monostearate (GMS) concentrations (1, 3, and 5 g/L). It was used as inducer in solid-state fermentation. A medium lacking GMS was used as control. Biomass production and enzyme productivity were higher in inducer-added (1 g/L) medium than in the control medium. T. harzianum produced constitutive and inducible cutinolytic esterase, in which production was enhanced by GMS. In GMS-added cultures, two bands with cutinolytic esterase activity (60 and 150 kDa approximately) were observed by zymography, which were not observed in control culture. GMS represents a promising inducer for cutinolytic esterase production by fungi. This research represents the first approach for the study of cutinolytic esterase production using a synthetic molecule as an inducer.

show abstract

“…In particular, Trichoderma harzianum has been described to be able to degrade the plasticizer di(2-ethyl hexyl) phthalate, which make plastics elastic, flexible and extensible (Aguilar-Alvarado et al, 2015). Some ascomycete fungi have been reported to be highly efficient phthalate-degrading organisms due to their secretion of enzymes such as esterase (Chhaya & Gupte, 2013;Aguilar-Alvarado et al, 2015;Bouchiat et al, 2015;Ahuactzin-Pérez, et al, 2016;Ferrer-Parra et al, 2018;González-Márquez et al, 2019a,b;Rios-González et al, 2019;González-Márquez et al, 2020;González-Márquez et al, 2021). The addition of surfactants in the medium is a common practice since it makes hydrophobic substrates available to the microorganism.…”

Section: Introductionmentioning

confidence: 99%

Tween 80-induced esterase production by Trichoderma harzianum in submerged fermentation: An esterase activity assay using α-naphthyl acetate as substrate

González-Marquez¹,

Sánchez²

2022

Mexjbiotechnol

Self Cite

View full text Add to dashboard Cite

Tween 80 is a surfactant that has been used for several purposes in microbial cultures. In bioremediation studies, the addition of surfactants in the medium is a common practice, since it makes hydrophobic substrates available to the microorganism. In this work, the influence of Tween 80 (100 or 400 μL/L) on the biomass production, protein content, esterase activities and, growth and enzymatic yield parameters of Trichoderma harzianum grown in submerged fermentation was evaluated. Assessments of esterase activities through biochemical tests and zymographic assays were carried out using α-naphthyl acetate as substrate. A single band with esterase activity was observed in the different concentrations of Tween 80 tested. However, 400 μL of Tween 80 induced higher esterase production (148.2 U/L) in T. harzianum as compared to that esterase activity (79.5 U/L) shown in the medium added with 100 μL of Tween 80 per liter. These results showed that high concentrations (e.g. 400 μL/L) of Tween 80 enhanced the esterase activity in T. harzianum. The single band with esterase activity revealed on zymograms suggests that this enzyme (of 38 kDa approx.) may be present in both forms as a constitutive as well as an inducible esterase, since it was observed in both glucose- and Tween 80-supplemented media.

show abstract

Induction of esterase activity during the degradation of high concentrations of the contaminant di(2-ethylhexyl) phthalate by Fusarium culmorum under liquid fermentation conditions

Cited by 9 publications

References 23 publications

Enhanced esterase activity during the degradation of dibutyl phthalate by Fusarium species in liquid fermentation

Enhanced esterase activity during the degradation of dibutyl phthalate by Fusarium species in liquid fermentation

Cutinolytic esterases are induced by growth of the fungus Trichoderma harzianum on glyceryl monostearate in solid-state fermentation

Tween 80-induced esterase production by Trichoderma harzianum in submerged fermentation: An esterase activity assay using α-naphthyl acetate as substrate

Contact Info

Product

Resources

About