Induction of Experimental Tetany in Cattle

Bohman, V. R.; Lesperance, A. L.; Harding, G.D.; Gruñes, D. L.

doi:10.2527/jas1969.29199x

Cited by 36 publications

(23 citation statements)

References 2 publications

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“…Because high concentrations of trans-aconitate coincided with the occurrence of grass tetany, ingestion of trans-aconitate has been suggested to play a role in the grass tetany syndrome in ruminants (Burau & Stout, 1965;Bohman, Lesperance, Harding & Grunes, 1969). However, because trans-aconitic acid, but not other organic acids, e.g.…”

Section: Introductionmentioning

confidence: 99%

Transport of tricarballylate by intestinal brush‐border membrane vesicles from steers

Wolffram

Zimmermann²,

Scharrer³

1993

Experimental Physiology

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SUMMARYTricarballylic acid is a non-metabolizable rumen bacterial fermentation product of the naturally occurring tricarboxylic acid trans-aconitic acid. The aim of the present study was to investigate intestinal absorption of tricarballylate using brush-border membrane vesicles (BBMVs) isolated from the proximal jejunum of steers by a Ca2+ precipitation method with subsequent differential centrifugation. Transport of tricarballylate was investigated indirectly (influence of tricarballylate on the uptake of "4C-labelled citrate) as well as directly (uptake of 3H-labelled tricarballylate). Citrate as well as tricarballylate uptake (at a concentration of 0 05 mmol 1-1) was strongly stimulated by an inwardly directed initial Na+ gradient. Furthermore, transport of both tricarboxylates under Na+ gradient conditions was clearly enhanced by lowering the extravesicular pH from 7-8 to 5-6. The imposition of an inwardly directed H+ gradient (pHout/pH1, = 5 6/7-8) further enhanced the intravesicular accumulation of citrate as well as of tricarballylate compared with pHout/pHin = 56/5 6. Unequivocal evidence for a common transport site for tricarballylate and citrate was obtained from 'cis-inhibition' and 'trans-stimulation' of Na+-dependent citrate uptake by tricarballylate. In further experiments the influence of different substances on the uptake of 3H-labelled tricarballylate was evaluated. Unlabelled tricarballylate, citrate, succinate as well as trans-and cis-aconitate significantly inhibited the accumulation of 3H-labelled tricarballylate by BBMVs. Tricarballylate uptake as a function of the tricarballylate concentration revealed a Na+-dependent saturable component (apparent kinetic parameters: maximal transport capacity (Vmax) = 119 pmol (mg protein)-' (3 s)-1; affinity constant (Km) = 0 097 mmol 1-1) and a Na+-independent diffusional component (diffusion constant: 169 nl (mg protein)-1 (3 s)-1). It is concluded that tricarballylate and citrate are transported across the intestinal brush-border membrane by a common, Na+-dependent transport mechanism. The stimulatory influence of a low extravesicular pH most probably indicates that the protonated forms of tricarboxylates are better transported than the trivalent species.

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Section: Introductionmentioning

confidence: 99%

Transport of tricarballylate by intestinal brush‐border membrane vesicles from steers

Wolffram

Zimmermann²,

Scharrer³

1993

Experimental Physiology

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“…Stout et al (1967) suggested that this acid was forming complexes with Mg which decreased the availability of Mgt } to the animal and that 1% trans-aconitic acid (172 mequiv/kg dry matter) was potentially toxic. Additional evidence for the role of organic acids in grass tetany was provided by Bohman et al (1969). who produced toxic symptoms in cattle by oral administration of KC1 with trans-aconitic acid or citric acid.…”

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confidence: 99%

Partition column chromatography for quantitating effects of fertilization on plant acids

Prior¹,

Gruñes²,

Patterson³

et al. 1973

J. Agric. Food Chem.

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A method for determining organic acid levels in biological materials by silica gel partition column chromatography (pcc) is described. It uses tetrabromophenolphthalein ethyl ester, which has E. high molar absorbency, for quantitating the acids in the column effluent. The concentrations of several organic acids were determined in two species of crested wheatgrass, Nordan (Agropyron ciesertorum) and Fairway (A. cristatum), grown. under different conditions of K fertilization. For comparison, aconitic, citric, and malic acids were also determined by polarography, spectrophotometry, and fluorometry, respectively. Results obtained by these methods agreed with the data obtained by pcc. The concentration of trans-aconitic acid averaged about 96 and 21 mequiv/kg of dry matter in Nordan and Fairway, respectively, when grown in the greenhouse without K. K fertilization (312 kg/ha) approximately doubled the trans-aconitic acid concentration in both species. Fairway contained 363 mequiv/kg of malic acid, while Nordan contained 280 mequiv/kg. K fertilization increased these concentrations to 611 and 446 mequiv/kg, respectively. Citric acid was increased by K fertilization, but there was no significant difference between species and in no case was the citric acid concentration greater than 80 mequiv/kg of dry matter.

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“…Sufficient quantities of cholesteryl esters could be isolated from liver, adrenal, testis, spleen, kidney cortex, and lipid droplets for GLC analysis (Table II). Unlike rat inner medulla, no cholesteryl esters could be detected in total lipid extracts of rabbit inner medullas (12,13), and only a small percentage of lipids isolated from lipid droplets were cholesteryl esters (Table IV). Polyunsaturation of cholesteryl esters found in most tissues was low and confined to, linoleic acid (18:2n-6).…”

Section: Resultsmentioning

confidence: 99%

“…During hydronephrosis or after administration of indometha-1presented in part at the Biochemistry-Biophysics Meeting, Minneapolis, 1974, and AOCS 48th Annual Fall Meeting, Philadelphia, 1974. cin, a prostaglandin synthetase and phosphodiesterase inhibitor, the number of lipid droplets and their trigly6eride content were observed to increase significantly (11). Extracts of inner medullas and lipid droplets, isolated by sucrose density centrifugation from rat and rabbit inner medullary homogenates, have been reported in preliminary communications to contain significant amounts of arachidonic acid, the precursor for prostaglandins E 2 and F2a (11)(12)(13)(14).…”

Section: Introductionmentioning

confidence: 94%

Analyses of renal medullary lipid droplets from normal, hydronephrotic, and indomethacin treated rabbits

Comai

Farber

Paulsrud

1975

Lipids

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Lipid droplets isolated from rabbit renal medullary tissue were analyzed and found to be composed of triglyceride and free fatty acids in a ratio of 2.9:1. These triglycerides were unique when compared to triglycerides of other rabbit tissues examined, in that they contained high percentages of octadecanoic acid (stearic acid, 9.8%), 5,8,11,14-eicosatetraenoic acid (arachidonic acid, 6.8%), and 7,10,13,16-docosatetraenoic acid (adrenic acid, 10%). Lipid droplet triglycerides were found to increase during experimental hydronephrosis and after administration of indomethacin, a prostaglandin synthetase and phosphodiesterase inhibitor. From gas liquid chromatography of fatty acid methyl esters of these triglycerides, it was determined that they were enriched further in their percent composition of 9,12-octadecadienoic acid (linoleic acid) and arachidonic acid, a prostaglandin precursor. The inverse relationship between lipid droplets and prostaglandin content in the inner medulla suggested a significant role of lipid droplet triglycerides as storage pools for prostaglandin precursors.

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Induction of Experimental Tetany in Cattle

Cited by 36 publications

References 2 publications

Transport of tricarballylate by intestinal brush‐border membrane vesicles from steers

Transport of tricarballylate by intestinal brush‐border membrane vesicles from steers

Partition column chromatography for quantitating effects of fertilization on plant acids

Analyses of renal medullary lipid droplets from normal, hydronephrotic, and indomethacin treated rabbits

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