Induction of fibronectin gene expression by inhibitors of protein phosphatase type 2B in normal and transformed fibroblasts

Rhew, Jung Hwa; Shin, Young Ah; Lee, Byung Heon; Park, Rang Woon; Kim, In San

doi:10.1038/emm.1999.12

Cited by 2 publications

(2 citation statements)

References 24 publications

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“…Those Ca 2+ -channel blockers presumably interfere with one or more Ca 2+ -dependent signalling events. In that context, it is worth noting that the calcineurin inhibitors cyclosporin A or tacrolimus inhibit matrix protein deposition by fibroblasts activated by TGFβ [136][137][138][139][140][141] or by basic fibroblast growth factor. One of these studies directly linked the beneficial effect of Ca 2+channel blockade to interference with signalling through the calcineurin-NFAT pathway 133 .…”

Section: Signaling Events?mentioning

confidence: 99%

Calcium Homeostasis and Ionic Mechanisms in Pulmonary Fibroblasts

Janssen

Mukherjee

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2015

Am J Respir Cell Mol Biol

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Fibroblasts are key cellular mediators of many chronic interstitial lung diseases, including idiopathic pulmonary fibrosis, scleroderma, sarcoidosis, drug-induced interstitial lung disease, and interstitial lung disease in connective tissue disease. A great deal of effort has been expended to understand the signaling mechanisms underlying the various cellular functions of fibroblasts. Recently, it has been shown that Ca(2+) oscillations play a central role in the regulation of gene expression in human pulmonary fibroblasts. However, the mechanisms whereby cytosolic [Ca(2+)] are regulated and [Ca(2+)] oscillations transduced are both poorly understood. In this review, we present the general concepts of [Ca(2+)] homeostasis, of ionic mechanisms responsible for various Ca(2+) fluxes, and of regulation of gene expression by [Ca(2+)]. In each case, we then also summarize the original findings that pertain specifically to pulmonary fibroblasts. From these data, we propose an overall signaling cascade by which excitation of the fibroblasts triggers pulsatile release of internally sequestered Ca(2+), which, in turn, activates membrane conductances, including voltage-dependent Ca(2+) influx pathways. Collectively, these events produce recurring Ca(2+) oscillations, the frequency of which is transduced by Ca(2+)-dependent transcription factors, which, in turn, orchestrate a variety of cellular events, including proliferation, synthesis/secretion of extracellular matrix proteins, autoactivation (production of transforming growth factor-β), and transformation into myofibroblasts. That unifying hypothesis, in turn, allows us to highlight several specific cellular targets and therapeutic intervention strategies aimed at controlling unwanted pulmonary fibrosis. The relationships between Ca(2+) signaling events and the unfolded protein response and apoptosis are also explored.

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Section: Signaling Events?mentioning

confidence: 99%

Calcium Homeostasis and Ionic Mechanisms in Pulmonary Fibroblasts

Janssen

Mukherjee

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2015

Am J Respir Cell Mol Biol

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“…The culture dishes were sterilized by UV exposure prior to use. Cells were cultured as previously described (Rhew et al, 1999). Approximately 1×10 4 human melanoma A375 cells (ATCC, Rockville, MD, USA) were seeded on the 25-mm glass coverslip of the custom culture dish and grown in 0.8-1.0 ml of RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 1% penicillinstreptomycin (Life Technologies, Gaithersburg, MD, USA).…”

Section: Cell Culturementioning

confidence: 99%

Monitoring transfected cells without selection agents by using the dual-cassette expression EGFP vectors

Kang

Lee²,

Schaus³

et al. 2001

Exp Mol Med

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Conventional methods of selecting gene transfected cells by toxic agents may yield ambiguous results.It is difficult to determine whether cell death is due to selection agents or gene transfection, owing to the substantial overlap of the time-courses for both effects. Therefore, to determine transfection-induced cell toxicity, the mammalian expression vector pEGFP-N1 (CLONTECH Lab., Palo Alto, CA, USA) has been modified to the dual-cassette expression vectors named pEGFP-Ks by the relocation of its EGFP expression cassette. We have precisely monitored the cells transfected with this vector on our custom culture dishes, thereby bypassing the need for selection agent or fluorescent cell sorting. This is a useful method to screen genes encoding potential toxic or useful proteins without performing undesirable selection agent and also can be used to monitor the transfected cells for various purposes, either the inhibition or proliferation of mammalian cells for applications in biotechnology.

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Induction of fibronectin gene expression by inhibitors of protein phosphatase type 2B in normal and transformed fibroblasts

Cited by 2 publications

References 24 publications

Calcium Homeostasis and Ionic Mechanisms in Pulmonary Fibroblasts

Calcium Homeostasis and Ionic Mechanisms in Pulmonary Fibroblasts

Monitoring transfected cells without selection agents by using the dual-cassette expression EGFP vectors

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