Induction of genuine autophagy by cationic lipids in mammalian cells

Man, Na; Chen, Yong; Zheng, Fang; Zhou, Wei; Wen, Longping

doi:10.4161/auto.6.4.11612

Cited by 63 publications

(46 citation statements)

References 34 publications

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“…In addition, Lipofectamine 2000 alone also exhibited higher LC3-II levels (Fig. 1b, Lane 2) compared to the non-treated control, which agreed with a recently published report about autophagy induction by cationic lipids [22]. However, the treatment with 200 nM Lipofectamine 2000/NC siRNA 1 complex resulted in a higher level of LC3-II than the treatment with the same amount of Lipofectamine 2000 or NC siRNA alone (Fig.…”

Section: Discussionsupporting

confidence: 91%

Effects of Lipofectamine 2000/siRNA Complexes on Autophagy in Hepatoma Cells

Zaro

et al. 2011

Mol Biotechnol

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Lipofectamine 2000 is commonly used for siRNA transfections. However, few studies have examined cellular responses to this delivery system. The purpose of this study is to evaluate the effect of siRNA transfection using Lipofectamine 2000 on cellular autophagy. Huh7.5 cells, stably-transfected to express GFP-LC3, were treated with Lipofectamine 2000/negative control siRNA (NC siRNA) complexes. At different time points after treatment, cells were lysed and analyzed by immunoblotting and fluorescence spectroscopy. Cells were also observed using confocal microscopy. An increase of endogenous LC3 lipidation, GFP-LC3 fluorescence, and autophagosomal puncta was observed in cells treated with Lipofectamine 2000/NC siRNA complexes. The kinetics of the increase of GFP-LC3 fluorescence correlated with the concentration of NC siRNA transfected, where 50, 100, and 200 nM NC siRNA caused a significant increase at 72, 48 and 24 hours, respectively, after transfection. A similar effect on the GFP-LC3 signal was also observed for cells treated with Lipofectamine 2000 complexed with two other NC siRNAs. Lipofectamine 2000-mediated transport of NC siRNAs led to an increase of autophagosomes in a dose- and time-dependent manner. Thus, this effect on cells should be taken into consideration when using this approach for intracellular delivery of siRNA.

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Section: Discussionsupporting

confidence: 91%

Effects of Lipofectamine 2000/siRNA Complexes on Autophagy in Hepatoma Cells

Zaro

et al. 2011

Mol Biotechnol

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“…8 Also, extensive LC3 dot formation, as a genuine autophagic response to stress, was induced in HeLa cell lines by treatment with cationic lipids. 9 The above work should be confirmed by large independent studies.…”

Section: Expansion Of the Studymentioning

confidence: 59%

The "stone-like" pattern of autophagy in human epithelial tumors and tumor-like lesions: An approach to the clinical outcome

Sivridis

Giatromanolaki²,

Zois³

et al. 2010

Autophagy

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“…Another common transfection carrier, cationic liposomes, is also known to cause autophagy (30,31), which was recently shown to be dependent on endocytosis as well (31).…”

Section: Cpp Treatment Causes Endosome Damage-here We Havementioning

confidence: 99%