Induction of Glandular Carcinomas of the Prostate in the Mouse

Horning, E. S.

doi:10.1016/s0140-6736(46)91139-7

Cited by 28 publications

(10 citation statements)

References 3 publications

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“…Examination of Table I shows, however, that donor lung implants impregnated with a carcinogen survive more readily and in greater numbers in older hosts than would be the case if the grafts did not contain any carcinogen. These results confirm previous experience with grafts of prostatic epithelium under the same conditions (Horning, 1949 (Horning, 1949) suggest that tissues which are normally under hormonal influence are much better tolerated as grafts in host mice of the same sex and -strain than tissues which are to a greater extent independent of hormone action.…”

Section: "supporting

confidence: 90%

“…The method of tumour induction consists in isolating small strips of lung from 6-12 weeks Strain A mice, impregnating the grafts with crystals of a carcinogen (2-acetylaminofluorene, 2-aminofluorene, or 20-methylcholanthrene) prior to implanting them subcutaneously into host mice of similar age and strain in a manner which has been described elswhere (Horning, 1947). Usually 4 subcutaneous grafts were made on each side of the abdomen of a single host mouse in order to have the implants growing under identical conditions.…”

Section: Techniquementioning

confidence: 99%

“…These indirect methods are often laborious, and tumour induction may take a considerable time.Recently the author (Horning, 1947) reported the induction of pulmonary tumours after relatively short periods of treatment by the direct application of carcinogens to adult lung tissue growing as subcutaneous homologous grafts in host animals. Further experiments have shown that success with this technique depends upon several factors, and the object of this paper is to describe additional observations.…”

mentioning

confidence: 99%

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Studies on the Induction of Lung Cancer in Mice

Es¹

1950

Br J Cancer

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LUNG tissue is unsuitable for direct injection of carcinogens, consequently pulmonary tumours in laboratory animals have hitherto been induced by applying the carcinogen at some remote site, by injecting it subcutaneously, intratracheally, intravascularly, intraperitoneally, or by including it in the diet. These indirect methods are often laborious, and tumour induction may take a considerable time.Recently the author (Horning, 1947) reported the induction of pulmonary tumours after relatively short periods of treatment by the direct application of carcinogens to adult lung tissue growing as subcutaneous homologous grafts in host animals. Further experiments have shown that success with this technique depends upon several factors, and the object of this paper is to describe additional observations. TECHNIQUE.The method of tumour induction consists in isolating small strips of lung from 6-12 weeks Strain A mice, impregnating the grafts with crystals of a carcinogen (2-acetylaminofluorene, 2-aminofluorene, or 20-methylcholanthrene) prior to implanting them subcutaneously into host mice of similar age and strain in a manner which has been described elswhere (Horning, 1947). Usually 4 subcutaneous grafts were made on each side of the abdomen of a single host mouse in order to have the implants growing under identical conditions. Small palpable tumours were obtained at intervals ranging from 6-12 weeks following grafting, but in order to examine the differences between the responses to individual carcinogens, grafts were fixed before tumours developed at intervals ranging from 1-5 weeks after implantation.Lung fragments from 61 weeks old Strain A mice were grafted, in some instances without the carcinogen, into host mice of similar age, and also into mice of 15 months age which were selected from stock mice of undetermined ancestry. This was done to find out if the survival of homologous grafts was dependent on employing a closely inbred strain, or whether it might be influenced by the age of the host.In another series of experiments a number of lung grafts were treated with 20-methylcholanthrene, half being implanted into the superficial fascia, and the remainder placed as usual between the skin and the abdominal wall. The host mice were again of the same age and strain, and it was thought that some information might be obtained as to whether rapid vascularization played any role in successful survival of the grafts.

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Section: "supporting

confidence: 90%

Section: Techniquementioning

confidence: 99%

mentioning

confidence: 99%

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Studies on the Induction of Lung Cancer in Mice

Es¹

1950

Br J Cancer

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“…Even the lens of the eye, in which spontaneous malignant growth has not been described, has been shown to be susceptible to neoplastic transformation (21). Recent evidence (22) suggests that embryonic tissues may be more susceptible to carcinogenic transformation than adult tissues (23). When embryonic tissues are" exposed to methylcholanthrene and then transplanted into animals of the same strain, neoplasms are observed within 20 to 40 days, a consider ably shorter period than is seen for tumors induced with this carcinogen in adult animals.…”

Section: Carcinogenesismentioning

confidence: 99%

Neoplastic Diseases

Shimkin¹

1950

Annu. Rev. Med.

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“…When such tissues as the lung, stomach, intes tine, skin, muscle and cartilage were treated in this way before inoculation some tumors were observed in the very brief latent period of from 20 to 40 days. Neoplasms of the prostate or lung were also obtained with adult tissues by the same technique (52,53) .…”

Section: The Induction Of Tumorsmentioning

confidence: 99%