Induction of hepatitis B virus-specific cytotoxic T lymphocytes response in vivo by filamentous phage display vaccine

Wan, Ying; Wu, Yuzhang; Bian, Jiang; Xiangzhi, Wang; Zhou, Weiping; Jia, Zhengcai; Tan, Yang; Zhou, Liyun

doi:10.1016/s0264-410x(00)00561-2

Cited by 57 publications

(44 citation statements)

References 30 publications

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“…It has been shown that filamentous phage particles can prime MHC class I-restricted cytotoxic T lymphocyte (CTL) responses in vivo [16][17][18]. Using MHC class I-peptide complex-specific antibodies, our data directly indicate that MHC class Ipeptide complexes are generated in MHC class IIpositive endocytic compartments.…”

Section: Introductionmentioning

confidence: 68%

Cross-presentation of phage particle antigen in MHC class II and endoplasmic reticulum marker-positive compartments

Wan

Zhou

et al. 2005

Eur. J. Immunol.

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It has been shown that exogenous antigens can access the MHC class I pathway of professional antigen-processing cells. However, details as to how the MHC class Ipeptide complex forms in the presentation pathway are still poorly understood. Here we used MHC class I-peptide-specific antibodies to investigate the formation and intracellular location of class I-peptide complexes in macrophages. We observed that the formation of class I-peptide complexes occurs within a few hours and lasts for another few hours on the cell surface of macrophages following loading with filamentous phage particles. The class I-peptide complexes in the process were colocalized with MHC class II molecules and endocytic system markers. Moreover, endosomal compartments containing class I-peptide complexes were found within intracellular organelles stained by DiOC6 and calnexin. In addition, the crosspresentation of phage particles was transporter associated with antigen processing (TAP)-dependent and sensitive to proteasome inhibitors and NH 4 Cl. These data suggest that endocytosed phage particles may be processed and cross-presented in organelles positive for phagosome and endoplasmic reticulum (ER) markers via a classical ER MHC class I loading mechanism.

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Section: Introductionmentioning

confidence: 68%

Cross-presentation of phage particle antigen in MHC class II and endoplasmic reticulum marker-positive compartments

Wan

Zhou

et al. 2005

Eur. J. Immunol.

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“…A disadvantage of using phage is the relatively weak T-cell help they provide. This issue may be addressed by modifying phage to include immunodominant foreign TCEs with known properties [39,40,59], either by direct fusion to the phage coat proteins or chemical conjugation to pVIII (our unpublished data).…”

Section: Modification Of Phage Carriers For Optimizing Immune Responsesmentioning

confidence: 99%

Filamentous phage as an immunogenic carrier to elicit focused antibody responses against a synthetic peptide

Houten

Zwick

Menéndez

et al. 2006

Vaccine

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Filamentous bacteriophage are widely used as immunogenic carriers for "phage-displayed" recombinant peptides. Here we report that they are an effective immunogenic carrier for synthetic peptides. The f1.K phage was engineered to have an additional Lys residue near the N-terminus of the major coat protein, pVIII, so as to enhance access to chemical cross-linking agents. The dimeric synthetic peptide, B2

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“…Low HBV-specific CTL responses in chronic HBV infection may favor the persistence of virus, whereas stimulation and expansion of HBV-specific CTL activity may assist elimination of HBV infection [1][2][3][4][5][6][7][8] . Natural HBV antigens contain some inappropriate epitopes and conserved amino acid sequences which might induce inappropriate immune responses and result in hepatic pathology and lesions.…”

Section: Hbv-specific Cd8mentioning

confidence: 99%

“…As in other infections with noncytopathic viruses, an MHC class I-restricted cytotoxic T lymphocytes (CTLs) response to endogenous HBV antigens is believed to be the major determinant for infected cell clearance, and HBV-specific CTL-mediated cytotoxicity plays the key role in controlling HBV infection and in the clearance of infected cells [1][2][3][4][5][6][7][8] . Natural HBV antigens contain generally inappropriate epitopes which could elicit T H1 /T H2 disequilibrium, immune deviation or immune deficiency, and the conserved amino acid sequences might also interfere with intercellular communication.…”

Section: Introductionmentioning

confidence: 99%

Therapeutic polypeptides based on HBcAg_18-27CTL epitope can induce antigen-specific CD₈⁺CTL-mediated cytotoxicity in HLA-A2 transgenic mice

Shi¹,

Wu²,

Jia³

et al. 2004

WJG

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Induction of hepatitis B virus-specific cytotoxic T lymphocytes response in vivo by filamentous phage display vaccine

Cited by 57 publications

References 30 publications

Cross-presentation of phage particle antigen in MHC class II and endoplasmic reticulum marker-positive compartments

Cross-presentation of phage particle antigen in MHC class II and endoplasmic reticulum marker-positive compartments

Filamentous phage as an immunogenic carrier to elicit focused antibody responses against a synthetic peptide

Therapeutic polypeptides based on HBcAg_18-27CTL epitope can induce antigen-specific CD₈⁺CTL-mediated cytotoxicity in HLA-A2 transgenic mice

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Induction of hepatitis B virus-specific cytotoxic T lymphocytes response in vivo by filamentous phage display vaccine

Cited by 57 publications

References 30 publications

Cross-presentation of phage particle antigen in MHC class II and endoplasmic reticulum marker-positive compartments

Cross-presentation of phage particle antigen in MHC class II and endoplasmic reticulum marker-positive compartments

Filamentous phage as an immunogenic carrier to elicit focused antibody responses against a synthetic peptide

Therapeutic polypeptides based on HBcAg18-27CTL epitope can induce antigen-specific CD8+CTL-mediated cytotoxicity in HLA-A2 transgenic mice

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Therapeutic polypeptides based on HBcAg_18-27CTL epitope can induce antigen-specific CD₈⁺CTL-mediated cytotoxicity in HLA-A2 transgenic mice