Induction of hepatocyte growth factor in human skin fibroblasts by epidermal growth factor, platelet-derived growth factor and fibroblast growth factor

Gohda, Eiichi; Matsunaga, Takaharu; Kataoka, Hirotoshi; Takebe, Takahiro; Yamamoto, Ichiro

doi:10.1016/1043-4666(94)90051-5

Cited by 90 publications

(54 citation statements)

References 28 publications

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“…We have speculated that one of the most likely candidates is a growth factor such as EGF or PDGF. This notion is supported by the fact that the extract stimulated proliferation of the cells and the fact that growth factors such as EGF, PDGF and bFGF induce HGF production in fibroblasts [25]. The notion is also consistent with our results showing that the HGF production-inducing activity and the cell growth-promoting activity of the extract were not separated by gel filtration (Fig.…”

Section: Discussionsupporting

confidence: 81%

“…Therefore, the active substance in the extract seems to be a non-proteinous macromolecule. Most of the macromolecular inducers of HGF are proteins such as growth factors and cytokines [24,25,27,28,30]. Non-proteinous macromolecular HGF inducers reported so far is heparin which is effective in MRC-5 cells and others [23].…”

Section: Discussionmentioning

confidence: 99%

“…Fig. 1 Next, we compared the effect of the extract of bitter melon pulp with the effects of other agents known to induce HGF production in human dermal fibroblasts [25,26]. The effect of the extract of bitter melon pulp was stronger than the effects of growth factors such as EGF (3 ng/ml) and PDGF (10 ng/ml) but weaker than the effect of cholera toxin (1 pM) (Fig.…”

Section: Induction Of Hgf Production By the Extract Of Bitter Melon Pulpmentioning

confidence: 99%

“…HGF production is induced in response to activation of PKA-and PKC-mediated pathways and is also induced by interleukin-1, tumor necrosis factor-α, interferon-γ, oncostatin-M, heparin, norepinephrine, staurosporine, a scatter factor-inducing factor, and growth factors such as epidermal growth factor (EGF) and platelet-derived growth factor (PDGF) [21][22][23][24][25][26][27][28][29][30]. To date, however, there have been no reports on the effects of plant extracts or juices on HGF production.…”

Section: Introductionmentioning

confidence: 99%

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Induction of hepatocyte growth factor production in human dermal fibroblasts and their proliferation by the extract of bitter melon pulp

et al. 2009

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Hepatocyte growth factor (HGF) is useful as a potential therapeutic agent for hepatic and renal fibrosis and cardiovascular diseases through inducing proliferation of epithelial and endothelial cells. HGF inducers may also be useful as therapeutic agents for these diseases. However, there have been no reports on induction of HGF production by plant extracts or juices. An extract of bitter melon (Momordica charantia L.) pulp markedly induced HGF production. There was a time lag of 72 h before induction of HGF production after the extract addition. Its stimulatory effect was accompanied by upregulation of HGF gene expression. Increases in mitogen-activated protein kinases (MAPKs) were observed from 72 h after the extract addition. Inhibitors of MAPKs suppressed the extract-induced HGF production. The extract also stimulated cell proliferation.Both activities for induction of HGF production and cell proliferation were eluted together in a single peak with 14 000 Da on gel filtration. The results indicate that bitter melon pulp extract induced HGF production and cell proliferation of human dermal fibroblasts and suggest that activation of MAPKs is involved in the HGF induction. Our findings suggest potential usefulness of the extract for tissue regeneration and provide an insight into the molecular mechanism underlying the wound-healing property of bitter melon.

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Section: Discussionsupporting

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Section: Induction Of Hgf Production By the Extract Of Bitter Melon Pulpmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Induction of hepatocyte growth factor production in human dermal fibroblasts and their proliferation by the extract of bitter melon pulp

et al. 2009

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“…For example, interleukin-1, one of growth factors such as epidermal growth factor (EGF) and platelet-derived growth factor (PDGF) are known to induce HGF production. 11,12) Chicoric acid, a caffeic acid derivative, isolated from Taraxacum which has been utilized as a traditional medicine for dyspepsia, heartburn, spleen and liver complaints, hepatitis, also proved to have accelerated ability on the production of HGF in normal human dermal fibroblasts. 13,14) Although these bioactive caffeic acid derivatives may have possibility to promote HGF production, there are few reports about their promoting activity.…”

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confidence: 99%

Induction of Hepatocyte Growth Factor Production in Human Dermal Fibroblasts by Caffeic Acid Derivatives

Kurisu

Nakasone

Miyamae

et al. 2013

Biological & Pharmaceutical Bulletin

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Hepatocyte growth factor (HGF) has mitogenic, motogenic, and morphogenic activities in epithelial cells. Induction of HGF production may be involved in organ regeneration, wound healing and embryogenesis. In this study, we examined the effects of caffeic acid derivatives including 4,5-di-O-caffeoylquinic acid (1) and acteoside (2) on HGF production in Neonatal Normal Human Dermal Fibroblasts (NHDF). Both 4,5-di-O-caffeoylquinic acid (1) and acteoside (2) significantly induced HGF production dose-dependent manner. To know the important substructure for HGF production activity, we next investigated the effect of the partial structure of these caffeic acid derivatives. From the results, caffeic acid (3) showed strong activity on the promotion of HGF production, while hydroxytyrosol (4) and quinic acid (5) didn't show any activity. Our findings suggest that the caffeoyl moiety of caffeic acid derivatives is essential for accelerated production of HGF. The compound which has the caffeoyl moiety may be useful for the treatment of some intractable organ disease.

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Levels of vascular endothelial growth factor, hepatocyte growth factor/scatter factor and basic fibroblast growth factor in human gliomas and their relation to angiogenesis

et al. 1999

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Angiogenesis is a possible target in the treatment of human gliomas. To evaluate the role of 3 growth factors, vascular endothelial growth factor (VEGF), hepatocyte growth factor/scatter factor (HGF/SF) and basic fibroblast growth factor (bFGF), in the angiogenic cascade, we determined their levels in extracts of 71 gliomas by enzyme‐linked immunosorbent assay (ELISA). The levels of bFGF were only marginally different between gliomas of World Health Organization (WHO) grade II (low grade) and grades III and IV (high grade). In contrast, the mean concentrations of VEGF were 11‐fold higher in high‐grade tumors and those of HGF/SF 7‐fold, respectively. Both were highly significantly correlated with microvessel density (p < 0.001) as determined by immunostaining for factor VIII‐related antigen. In addition, VEGF and HGF/SF appeared to be independent predictive parameters for glioma microvessel density as determined by multiple regression analysis. We measured the capacity of all 3 factors to induce endothelial tube formation in a collagen gel. In this assay, bFGF was found to be an essential cofactor with which VEGF as well as HGF/SF were able to synergize independently. According to the concentrations of angiogenic factors, extracts from high‐grade tumors were significantly more potent in the tube formation assay than the low‐grade extracts (p = 0.02). Adding neutralizing antibodies to bFGF, VEGF and HGF/SF together with the extracts, tube formation was inhibited by up to 98%, 62% and 54%, respectively. Our findings suggest that bFGF is an essential cofactor for angiogenesis in gliomas, but in itself is insufficient as it is present already in the sparsely vascularized low‐grade tumors. Upon induction of angiogenesis in high‐grade tumors, bFGF may synergize with rising levels of not only VEGF but possibly also with HGF/SF, which appears here to be an independent angiogenic factor. Int. J. Cancer (Pred. Oncol.) 84:10–18, 1999. © 1999 Wiley‐Liss, Inc.

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Induction of hepatocyte growth factor in human skin fibroblasts by epidermal growth factor, platelet-derived growth factor and fibroblast growth factor

Cited by 90 publications

References 28 publications

Induction of hepatocyte growth factor production in human dermal fibroblasts and their proliferation by the extract of bitter melon pulp

Induction of hepatocyte growth factor production in human dermal fibroblasts and their proliferation by the extract of bitter melon pulp

Induction of Hepatocyte Growth Factor Production in Human Dermal Fibroblasts by Caffeic Acid Derivatives

Levels of vascular endothelial growth factor, hepatocyte growth factor/scatter factor and basic fibroblast growth factor in human gliomas and their relation to angiogenesis

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