Induction of heterozygous and homozygous diploid gynogenesis in Betta splendens (Regan) using hydrostatic pressure

Kavumpurath, Soosamma; Pandian, T. J.

doi:10.1111/j.1365-2109.1994.tb00569.x

Cited by 12 publications

(6 citation statements)

References 28 publications

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“…This species has an XX/XY type of sex determination mechanism, on the basis of apparent female homogamety. This conclusion is further supported by the evidence from hormally feminized fish (Kavumpurath & Pandian 1993) and gynogenesis (Kavumpurath & Pandian 1994).…”

Section: Discussionmentioning

confidence: 64%

Masculinization of fighting fish, Betta splendens Regan, using synthetic or natural androgens

Kavumpurath

Pandian

1994

Aquaculture Res

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The effects of two synthetic androgens, 17a-methyltestosterone (5-50mg/kg food), 19-nor-ethynyltestosterone (2-50 mg/kg food) and two natural androgens, 11 ketotestosterone (10-60mg/kg food) and androstenedione (20-100mg/kg food) were investigated in the fighting fish, Betta splendens Regan. Androgens were administered for 40 days from the first day of feeding. Masculinization occurred in 100% of individuals fed 8, 15, 60, 90 mg/kg food 19-nor-ET, 17a-MT, 11-KT and AT respectively. Mortality due to treatment of natural steroids was significantly less than that with the synthetic steroids. Sex-reversed males were sexually functional and their genotype was identified by progeny testing. Sex-reversed males produced 100% female monosex when mated with normal females, indicating that the mechanism of sex determination in this fish is homogametic female and heterogametic male.

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Section: Discussionmentioning

confidence: 64%

Masculinization of fighting fish, Betta splendens Regan, using synthetic or natural androgens

Kavumpurath

Pandian

1994

Aquaculture Res

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“…Therefore, to ensure that paternal chromosomes are not transmitted to the next generation, sperm from a different fish species, given that no viable hybrids are produced, was used (Chourrout, 1986;Varadaraj, 1990;Sugama et al, 1990;Kavumpurath & Pandian, 1994;Smoker et al, 1995;Linhart et al, 1995;Mims et al, 1997;Fujioka, 1998;Gomelsky et al, 1998;Kawamura, 1998). Thus, individual embryos arising accidentally from intact sperm will be eliminated by lethal hybridization (Chourrout, 1986).…”

Section: Introductionmentioning

confidence: 99%

Induction of gynogenesis and gonad development in the muskellunge

Rlnchard

Garcia-Abiado

Dąbrowski

et al. 2002

Journal of Fish Biology

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Muskellunge Esox masquinongy eggs activated with UV-irradiated yellow perch Perca flavescens sperm were subjected to heat shock at 31 1 C for 6 min, 20 min after fertilization in three experiments. Survival at eyed stage was 1·7 1·6, 6·8 4·8 and 2·3 0·5% in experiments I, II and III, respectively. After rearing the gynogenetic muskellunge in troughs and then in ponds, the sex ratio of gynogens in experiment I did not significantly (P>0·05) differ from the expected ratio 0 : 1 (male : female), however, one male and one intersex were observed. In experiments II and III, the sex ratio of gynogens differed significantly from expected (0 : 1). Three months after hatching, the growth of females did not significantly (P>0·05) differ among control and gynogenetic groups, whereas male growth was significantly (P<0·01) higher in the control v. gynogenetic group. The histological structures of the gynogenetic fish gonads in both sexes were similar to those described in the gonads of control muskellunge. After the overwintering period, signs of active spermatogenesis were observed in the testis of 1+ year old gynogenetic fish, whereas ovaries contained only oocytes at the perinucleolar stage. At this stage, plasma sex steroids testosterone (T) and oestradiol-17 (E2) cannot be used to discriminate the sex of gynogenetic muskellunge, as intersex, male and female fish had similar levels of T and E2.

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“…Artificial gynogenesis has been induced in a number of fish species, e.g. rainbow trout [8], tilapia [9], fighting fish [10], white bass [11], red crucian carp [12], and Japanese crucian carp [13]. The main advantage of gynogenesis using diploid eggs is that it avoids the treatment for doubling the chromosomes, thus not only simplifying the technological process, but also increasing the survival rate of the gynogenetic fish.…”

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confidence: 99%

Distant hybridization leads to different ploidy fishes

Liu

2010

Sci. China Life Sci.

188

136

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Distant hybridization makes it possible to transfer the genome of one species to another, which results in changes in phenotypes and genotypes of the progenies. This study shows that distant hybridization or the combination of this method with gynogenesis or androgenesis lead to different ploidy fishes with genetic variation, including fertile tetraploid hybrids, sterile triploid hybrids, fertile diploid hybrids, fertile diploid gynogenetic fish, and their derived progenies. The formations of the different ploidy fishes depend on the genetic relationship between the parents. In this study, several types of distant hybridization, including red crucian carp (Carassius auratus red var.) (2n=100, abbreviated as RCC) (female) x common carp (Cyprinus carpio L.) (2n=100, abbreviated as CC) (male), and RCC (2n=100) (female) x blunt snout bream (Megalobrama amblycephala) (2n=48, abbreviated as BSB) (male) are described. In the distant hybridization of RCC (female) x CC (male), bisexual fertile F(3)-F(18) allotetraploid hybrids (4n=200, abbreviated as 4nAT) were formed. The diploid hybrid eggs and diploid sperm generated by the females and males of 4nAT developed into diploid gynogenetic hybrids and diploid androgenetic hybrids, respectively, by gynogenesis and androgenesis, without treatment for doubling the chromosome. Improved tetraploid hybrids and improved diploid fishes with genetic variation were derived from the gynogenetic hybrid line. The improved diploid fishes included the high-body RCC and high-body goldfish. The formation of the tetraploid hybrids was related to the occurrence of unreduced gametes generated from the diploid hybrids, which involved in premeiotic endoreduplication, endomitosis, or fusion of germ cells. The sterile triploid hybrids (3n=150) were produced on a large scale by crossing the males of tetraploid hybrids with females of diploid fish (2n=100). In another distant hybridization of RCC (female) x BSB (male), different ploidy fishes were obtained, including diploid bisexual fertile natural gynogenetic fish (2n=100), sterile triploid hybrids (3n=124), and bisexual fertile tetraploid hybrids (4n=148). Furthermore, two kinds of pentaploid hybrids (5n=172 and 5n=198) were formed. The biological characteristics and the mechanisms of formation of the different ploidy fish were compared and discussed at the cellular and molecular level. The results indicated distant hybridization or the combination of this method with gynogenesis or androgenesis affects the formation of different ploidy fish with genetic variation.

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Induction of heterozygous and homozygous diploid gynogenesis in Betta splendens (Regan) using hydrostatic pressure

Cited by 12 publications

References 28 publications

Masculinization of fighting fish, Betta splendens Regan, using synthetic or natural androgens

Masculinization of fighting fish, Betta splendens Regan, using synthetic or natural androgens

Induction of gynogenesis and gonad development in the muskellunge

Distant hybridization leads to different ploidy fishes

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