Induction of high titers of anti-IgE by immunization of inbred mice with syngeneic IgE.

Nisonoff²

1990

Self Cite

Inoculation of syngeneic IgE into 2-to 12-day-old mice results in prolonged synthesis of anti-IgE antibodies without further challenge. These anti-IgE antibodies may be largely responsible for the long-term inhibition of synthesis of IgE that is known to result from a perinatal challenge with IgE. This conclusion is supported by the effect of passive inoculation of syngeneic polyclonal anti-IgE antibodies into young mice, which similarly results in selective inhibition of IgE synthesis. Further evidence is the close relationship between the age dependency of IgE-induced inhibition of subsequent IgE synthesis and the ability of IgE to induce anti-IgE antibodies. IgE synthesis was monitored at the level of secretion by B cells as well as serum IgE levels and IgE antibody responses.

Section: Methodsmentioning

confidence: 99%

“…The loss of responsiveness was attributed to tolerization of T cells by endogenous IgE. That B cells are not tolerant was shown by the capacity of mice of any age to produce anti-IgE in response to a keyhole limpet hemocyanin (KLH)-IgE conjugate (2). T cells can also be tolerized on the day of birth by i.p.…”

mentioning

confidence: 95%

Inhibition of IgE synthesis by anti-IgE: role in long-term inhibition of IgE synthesis by neonatally administered soluble IgE.

Nisonoff²

1990

Self Cite

“…The immune tolerance of adult mice to endogenous IgE resides in their T cells. The responsiveness of B cells is shown by the ability of mice to produce high titers of serum antibodies (up to 1 mg/ml) specific for isotypic determinants of IgE when challenged with a keyhole limpet hemocyanin (KLH) conjugate of syngeneic monoclonal IgE (3). KLH evidently provides the necessary T-cell help because adult mice do not respond to unconjugated IgE.…”

Section: Introductionmentioning

confidence: 99%

IgE-secreting cells in the thymus: correlation with induction of tolerance to IgE.

Nisonoff

1992

Self Cite

“…Recently we reported the induction of high titers (to 1 mg/ ml) of syngeneic anti-IgE with isotypic specificity in adult A/J mice (1). The immunogen used was a copolymer of keyhole limpet hemocyanin (KLH) with an A/J monoclonal antibody (mAb) of the IgE class.…”

mentioning

confidence: 99%

“…Wells of microtiter plates were coated with affinity-purified rabbit anti-IgD (TEPC-1017; 1 ,ug/ml), saturated with 2.5% horse serum, and then exposed to varying dilutions of serum. Development was done with '25I-labeled affinity-purified rabbit anti-IgD (40 ng in 0.1 ml).…”

mentioning

confidence: 99%

Induction and properties of syngeneic murine anti-immunoglobulin D.

Thorbecke²,

Nisonoff³

1988

Self Cite

High titers of autoantibodies directed to isotypic determinants of IgD were produced by inoculation of syngeneic monoclonal IgD, conjugated covalently to keyhole limpet hemocyanin, into adult or neonatal inbred mice. Antiidiotypic antibodies were induced at the same time. The average affinity of the mouse antibodies (Ka 9 107 M-1) is similar to that of rabbit anti-IgD and of syngeneic anti-IgE induced by the same procedure. Results indicate that B cells of the mice are not tolerant to serum IgD and that tolerance is maintained at the level of T cells. Direct interaction of the syngeneic anti-IgD with cell-surface IgD was minimal, and there was no convincing evidence that cell-surface IgD was down-regulated in the anti-IgD-producing mice. Further studies, preferably employing monoclonal anti-IgD, are required to determine whether epitopes on cell-surface IgD can be recognized by syngeneic anti-IgD. The ability to generate in vivo high titers of anti-IgD should facilitate the production of such monoclonal antibodies.