Induction of Hyphae in Cultures of Pityrosporum by Cholesterol and Cholesterol Esters

Porro, M. Nazzaro; Passi, S.; Caprilli, F.; Mercantini, R.

doi:10.1111/1523-1747.ep12687967

Cited by 69 publications

(18 citation statements)

References 17 publications

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“…As demonstrated by the control noninoculated CSSS, the underneath face of CSSS which is offered to Malassezia growth in the bioassay is normally free of these yeasts. It has been previously shown that these fungi can grow after inoculation on lipid-enriched human stratum corneum [36,37,40]. We presently confirm this feature and the potent anti-Malassezia activity of topically applied ketoconazole.…”

Section: Discussionsupporting

confidence: 85%

Corneofungimetry Bioassay on <i>Malassezia</i> spp. under Ketoconazole and Desonide Influences

Piérard-Franchimont

Vroome²,

Cauwenbergh³

et al. 2005

Skin Pharmacol Physiol

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Background: Glucocorticoids can boost some Malassezia-driven dermatoses. However, both antifungals and topical corticosteroids improve lesions of seborrheic dermatitis. Objective: To revisit the topical activity of the antifungal ketoconazole and the corticosteroid desonide on Malassezia growth on human stratum corneum. Material and Methods: The computer-assisted corneofungimetry bioassay was used to compare the growth of M. furfur, M. globosa and M. restricta on human stratum corneum coated with olive oil. Four blinded gel formulations were tested. They contained either 2% ketoconazole, 0.05% desonide or a combination of 2% ketoconazole and 0.05% desonide; one gel was unmedicated. Untreated stratum corneum and specimens coated with a 2% ketoconazole cream were used as negative and positive comparators, respectively. A total of 45 samples (15 M. furfur, 15 M. globosa, and 15 M. restricta) were used for each test formulation in this randomized, double-blind study. Results: The 2% ketoconazole gel and cream and the combination of 2% ketoconazole and 0.05% desonide formulation abated similarly and significantly the M. furfur, M. globosa and M. restricta growth. The 3 species were similarly sensitive to these formulations. By contrast, no significant inhibitory effect was yielded by the 0.05% desonide gel and the vehicle. Conclusion: The presence of 0.05% desonide does not impair or improve the Malassezia susceptibility to 2% ketoconazole when growing on lipid-enriched human stratum corneum.

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Section: Discussionsupporting

confidence: 85%

Corneofungimetry Bioassay on <i>Malassezia</i> spp. under Ketoconazole and Desonide Influences

Piérard-Franchimont

Vroome²,

Cauwenbergh³

et al. 2005

Skin Pharmacol Physiol

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“…In vitro , the addition of glucose or asparagine to the culture medium promotes growth of the yeast 59 . Cholesterol and cholesterol esters added to culture also stimulate the induction of hyphae 61 …”

Section: Immune Response To Malassezia In Pityriasis Versicolormentioning

confidence: 99%

Pityriasis versicolor

Gupta

Bluhm

Summerbell

2002

Acad Dermatol Venereol

153

168

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“…It was also noted that some of media components can stimulate in vitro the formation of mycelium by Malassezia sp ., for example Porro et al . obtained the growth of Malassezia sp . hyphal by adding cholesterol and cholesterol esters to the medium.…”

Section: Discussionmentioning

confidence: 99%

Comparison of lipid profiles of Malassezia pachydermatis strains isolated from dogs with otitis externa and without clinical symptoms of disease

Czyżewska

Siemieniuk

Pyrkowska

et al. 2015

Mycoses

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Malassezia pachydermatis can cause infections of the skin and mucous membranes, especially in animals. It becomes a problem also in medicine. It is considered that metabolic disorders as well as hormonal and immunological status of the host promote diseases caused by M. pachydermatis. Here we consider whether specific features of fungi could also favour infections. We checked whether there are differences in lipid profiles between strains obtained from dogs with otitis externa and strains obtained from healthy dogs. Lipid profiles were determined using thin layer chromatography and gas chromatography-mass spectrometry. All analyses were carried out on 32 strains derived from dogs with otitis externa and 31 strains isolated from dogs without symptoms of disease. The results show that strains isolated from dogs without symptoms of otitis externa are characterised by a higher content of fatty acids. They contain significantly more behenic and lignoceric acids on medium without addition of lipids, and more oleic acid and total monounsaturated fatty acids on medium with lipids supplementation. These strains have also a higher content of esters of ergosterol and triglycerides. Data obtained show differences which may be specific features of M. pachydermatis-specific strains related to the ability of infection, which could be not directly related of the host condition.

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Induction of Hyphae in Cultures of Pityrosporum by Cholesterol and Cholesterol Esters

Cited by 69 publications

References 17 publications

Corneofungimetry Bioassay on <i>Malassezia</i> spp. under Ketoconazole and Desonide Influences

Corneofungimetry Bioassay on <i>Malassezia</i> spp. under Ketoconazole and Desonide Influences

Pityriasis versicolor

Comparison of lipid profiles of Malassezia pachydermatis strains isolated from dogs with otitis externa and without clinical symptoms of disease

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