Induction of IFNT-Stimulated Genes by Conceptus-Derived Exosomes during the Attachment Period

Nakamura, Keigo; Kusama, Kaoru; Bai, Rulan; Sakurai, ﻿Toshihiro; Isuzugawa, Kazuto; Godkin, James D.; Suda, Yoshihito; Imakawa, Kazuhiko

doi:10.1371/journal.pone.0158278

Cited by 87 publications

(107 citation statements)

References 45 publications

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“…There is an upregulation of the AKR1B1 protein in the ovine conceptus at Days 15 and 17 of pregnancy (Nakamura et al, 2016), and AKR1B1 is identified as the most potent PGFS in the bovine endometrium (Lacroix Pépin, Chapdelaine, & Fortier, 2013). Dimer formation exists in the aldo-keto reductase 2 (AKR2), AKR6, and AKR7 families (Penning, 2003), and AKR1B1 may also form a dimer with higher activity than the monomer.…”

Section: It Is Through Transcervical Intrauterine (Body Of the Uterus)mentioning

confidence: 99%

Expression profiles of interferon‐stimulated gene 15 and prostaglandin synthases in the ovine lymph nodes during early pregnancy

Yang

Wang

Liu

et al. 2018

Molecular Reproduction Devel

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Lymph nodes are distributed all over the body and are part of the lymphatic system. The interferon‐stimulated gene 15 kDa protein (ISG15) and prostaglandins (PGs) are involved in the establishment of pregnancy and are expressed in the uterus during early pregnancy in sheep. In this study, the ovine lymph nodes were obtained on Day 16 of the estrous cycle, and Days 13, 16, and 25 of pregnancy, and the expression of ISG15 and PG synthases, including cyclooxygenase 1 (COX‐1), COX‐2, prostaglandin E (PGE) synthase (PTGES), and a PGF synthase (aldo‐keto reductase family 1, member B1, AKR1B1) were detected by quantitative real‐time polymerase chain reaction, western blot analysis, and immunohistochemistry analysis. Our results showed that there were peaks in the expression of mRNAs and the proteins of ISG15, COX‐1, COX‐2, PTGES, and AKR1B1 in the lymph nodes during early pregnancy and that the COX‐2 and AKR1B1 proteins were limited to the subcapsular sinus and lymph sinuses. In conclusion, the ISG15, COX‐1, COX‐2, PTGES, and AKR1B1 were upregulated in the maternal lymph nodes, which may be beneficial for the development of conceptus, maternal systemic immunoregulation, and anti‐luteolysis during early pregnancy in sheep.

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Section: It Is Through Transcervical Intrauterine (Body Of the Uterus)mentioning

confidence: 99%

Expression profiles of interferon‐stimulated gene 15 and prostaglandin synthases in the ovine lymph nodes during early pregnancy

Yang

Wang

Liu

et al. 2018

Molecular Reproduction Devel

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“…Extracellular vesicles have been identified in vivo in body fluids, including amniotic fluid, urine, and blood, and isolated from uterine fluid in sheep and women [18, 20, 21, 43, 44]. Furthermore, recent publications have demonstrated that EVs are released from human endometrial epithelial cells [18, 37, 44–46], extravillous and villous trophoblast cells [47], and primary trophoblast from term placenta [44, 48]. …”

Section: Extracellular Vesiclesmentioning

confidence: 99%

Extracellular Vesicles in the Intrauterine Environment: Challenges and Potential Functions

Nguyen

Simpson

Salamonsen

et al. 2016

Biology of Reproduction

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Extracellular vesicles (EVs), including exosomes (30–150 nm) and microvesicles (100–1500 nm), play important roles in mediating cell-cell communication. Such particles package distinct cargo elements, including lipids, proteins, mRNAs, microRNAs, and DNA, that vary depending on the cell of origin and its phenotype. This cargo can be horizontally transferred to target cells where its components can reprogram the recipient cell to modify its function. EVs have been identified within the uterine cavity of women, sheep, and mice, where they contribute to the microenvironment of sperm transport, and of blastocyst and endometrial preparation for implantation. It is likely that exosomes and microvesicles carry different cargo and coordinate different roles in this intrauterine environment. Understanding and defining these subtypes of EVs is important for future functional studies and clinical translation. Here we critically review the various purification and validation procedures for extracellular vesicle analysis and discuss what is known of endometrial-derived exosome cargo and of their hormonal regulation. The current knowledge of the functions of uterine exosomes, with respect to sperm transport and function, and of their actions on trophectodermal cells to promote implantation are summarized and evaluated in their physiological context. Given the potential importance of this form of cell-cell interactions within the reproductive tract, the critical issues discussed will guide new insights in this rapidly expanding field.

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“…For quantitative real-time PCR (qPCR) analyses, isolated RNA (total 250 ng) was reverse-transcribed to cDNA using ReverTra Ace qRNA RT Kit (Toyobo, Osaka, Japan), and the resulting cDNA (RT template) was stored at 4°C until use [21]. …”

Section: Methodsmentioning

confidence: 99%

Increase in complement iC3b is associated with anti-inflammatory cytokine expression during late pregnancy in mice

et al. 2017

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Immunological tolerance between fetal allograft and mother is crucial for pregnancy establishment and maintenance; however, these mechanisms particularly those during the latter part of pregnancy have not been definitively elucidated. The aim of this study was to examine the presence and potential function of innate immunity characteristic to the middle to late pregnancy. We first characterized up-regulated proteins in decidua from day 11 pregnant (P11) mice using 2D-PAGE, followed by MALDI-TOF/MS analysis. These analyses identified increased complement component 3 (C3) and its derivatives in P11 decidua. We then found that in the decidual tissues, C3 mRNA increased on P15 and remained high on P19. C3 is converted to C3b and then iC3b by complement component factor I (Cfi) and complement receptor 1-like protein (Crry), both of which were present in P19 placentas. In addition, iC3b proteins and its receptor CR3 (Cd11b/Cd18) in decidual and placental tissues increased toward the latter phase of pregnancy. Moreover, CR3 subunit CD11b protein was predominantly localized to spongiotrophoblast layer in the P19 placenta. Because iC3b is known to induce anti-inflammatory cytokine production, the analysis was extended to examine changes in pro- and anti-inflammatory cytokines, Il12, Il10, and Tgfb1. Il12 expression decreased in P15 and P19 placenta, while high mRNA expression of Il10 and Tgfb1 was found in P19 placental tissues. Furthermore, placental Il10 and Tgfb1 mRNAs were down-regulated when pregnant mice were treated with an anti-C3 antibody, detecting C3, C3b and iC3b. These results indicated that C3 derivatives, in particular, iC3b and its receptor CR3 were up-regulated at the fetal-maternal interface, and suggest that iC3b may regulate the placental expression of anti-inflammatory cytokines, IL10 and TGFB1, during the latter phase of pregnancy.

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Induction of IFNT-Stimulated Genes by Conceptus-Derived Exosomes during the Attachment Period

Cited by 87 publications

References 45 publications

Expression profiles of interferon‐stimulated gene 15 and prostaglandin synthases in the ovine lymph nodes during early pregnancy

Expression profiles of interferon‐stimulated gene 15 and prostaglandin synthases in the ovine lymph nodes during early pregnancy

Extracellular Vesicles in the Intrauterine Environment: Challenges and Potential Functions

Increase in complement iC3b is associated with anti-inflammatory cytokine expression during late pregnancy in mice

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