Induction of immune and adjuvant immunoglobulin G responses in mice by Brucella lipopolysaccharide

Moreno, Edgardo; Kurtz, R S; Berman, David T.

doi:10.1128/iai.46.1.74-80.1984

Cited by 27 publications

(24 citation statements)

References 28 publications

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“…Alternatively, LPS-EC is known to be less effective in stimulating macrophages in C3H/HeJ mice than in other mouse strains (31), and this may have reduced the antibody responses. These results are in agreement with previous studies which showed that LPS-BA could induce anti-LPS antibodies in C3H/HeJ B cells, whereas LPS-EC was less effective (20,21). The protein associated with the LPS-BA used in this study may have provided an adjuvant effect as described by others for other LPS preparations (22,30).…”

Section: Discussionsupporting

confidence: 93%

“…This distinguishes TNP-LPS-BA from the TI-2 antigen TNP-Ficoll, which could not induce responses in these mice, allowing us to classify LPS-BA as a TI-1 carrier. As expected (18,21,26), the known TI-1 antigens TNP-BA and TNP-LPS-EC and the TD antigen DNP-KLH were all effective in triggering anti-TNP immunoglobulin responses in these mice.…”

Section: Discussionsupporting

confidence: 85%

“…Although LPS-BA has been shown previously to induce anti-LPS antibody responses (20,21), it has not been used as a carrier for other determinants. In order to determine whether TNP-LPS-BA behaves as a TI-1 antigen, BALB/c, athymic, and CBA/N mice were studied.…”

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confidence: 99%

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Lipopolysaccharide from Brucella abortus behaves as a T-cell-independent type 1 carrier in murine antigen-specific antibody responses

et al. 1993

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In order to determine the carrier nature of lipopolysaccharide from Brucella abortus (LPS-BA) in evoking humoral responses, normal and immunodeficient mice were immunized with trinitrophenyl (TNP)-conjugated LPS-BA (TNP-LPS-BA) and the responses were compared with those to known T-dependent and T-independent antigens. TNP-LPS-BA, like T-independent type 1 (TI-1) antigens such as TNP-BA and TNP-LPS from Escherichia coli (TNP-LPS-EC), generated anti-TNP responses in BALB/c, athymic BALB/c nulnu, and CBAIN mice. In contrast, N-2,4-dinitrophenyl-j8-alanylglycylglycyl-substituted keyhole limpet hemocyanin, a typical T-dependent antigen, was not immunogenic in athymic mice, and TNP-Ficoll (T-independent type 2) was ineffective in eliciting humoral responses in CBA/N mice. These results indicate that LPS from B. abortus acts as a TI-i carrier in generating antibody responses. In C3H/HeJ mice, TNP-LPS-BA generated higher-titer immunoglobulin Gi (IgGI), IgG2a, and IgG2b anti-TNP antibodies than TNP-LPS-EC. Compared with those from BALB/c mice, pure resting B cells isolated from C3H/HeJ mice exhibited a 30-fold lower proliferative response to LPS-EC, whereas the LPS-BA response was reduced to a lesser extent (5-fold). This suggests that the disparity observed in antibody titers was due to different abilities of LPS from B. abortus and E. coli to stimulate C3H/HeJ B cells. The ability of LPS from B. abortus to act as a carrier in generating humoral immune responses indicates that LPS-BA can be substituted for whole B. abortus organisms in vaccine development. * Corresponding author. polysaccharides such as TNP-Ficoll are TI-2 antigens. Typically, TI-1 and TI-2 antigens are able to induce IgM antibody production in athymic nude mice, whereas a TD antigen, e.g., DNP-KLH, will induce little or no antibody

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 85%

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Lipopolysaccharide from Brucella abortus behaves as a T-cell-independent type 1 carrier in murine antigen-specific antibody responses

et al. 1993

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“…Subunit vaccines have been used but are not highly effective in protecting cattle from subsequent infection and disease (8,41). Factors which usually influence the development of immunity against brucellosis in mice are related to the nature and dose of immunogen and the manner in which the immunizing epitopes are presented to the host's immune surveillance system (2,3,5,10,19,20,27,31,32). The overall immunity induced involves both cell-mediated and humoral antibody effects, which probably act together in preventing and eliminating infection.…”

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confidence: 99%

Alteration of protective and serologic responses in BALB/c mice vaccinated with chemically modified versus nonmodified proteins of Brucella abortus 19

Pugh

Tabatabai

1994

Infect Immun

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A study was conducted to determine whether the covalent chemical modification of BruceUla abortus 19 salt-extractable proteins (BCSP) and BCSP derivatives would modulate the immune responses in BALB/c mice. Salt-extractable proteins BCSP 0-70 and BCSP 70-100 were modified with acetoacetic anhydride, and recombinant proteins rBCSP20 (20 kDa), rBCSP31 (31 kDa), and rBCSP45 (45 kDa) were modified with succinic and dodecanoyl anhydrides. Four weeks after mice were vaccinated with the different preparations, principal and control mice were challenge exposed with a virulent culture of B. abortus 2308, and mice were necropsied 2 weeks later. Serum samples were obtained immediately before mice were challenge exposed and at necropsy. Sera were tested for specific immunoglobulin M (IgM) and G (IgG) antibodies by using an enzyme-linked immunosorbent assay. Acylation decreased the immune responses (increased IgG antibodies and reduced spleen CFU and splenomegaly) induced by both BCSP 0-70 and BCSP 70-100. Modification of the recombinant proteins by dodecanoyl and succinic anhydrides had no effect on the protection induced; however, the IgG serologic responses to the homologous and heterologous proteins were altered. Monophosphoryl lipid A markedly enhanced the immunogenicity of BCSP 0-70.

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“…We have shown previously that the immunogenic and adjuvant properties of preparations of Brucella abortus endotoxin were different from those of lipopolysaccharide (LPS) from Escherichia coli (13). Consistent with previous reports (16,22), E. coli LPS did not stimulate significant primary or seondary specific antibody responses in LPShyporesponsive C3H/HeJ mice and induced the production of immunoglobulin M (IgM) and only low levels of IgG antibodies in congenic LPS-responsive C3H/HeAu mice, as well as in euthymic and athymic BALB/c mice.…”

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Influence of endotoxin-protein in immunoglobulin G isotype responses of mice to Brucella abortus lipopolysaccharide

Kurtz¹,

Berman²

1986

Infect Immun

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Brucella abortus endotoxin preparations, containing approximately 5 to 6% protein, induce strong immune and adjuvant immunoglobulin G (IgG) responses as compared with Escherichia coli endotoxin preparations, with equivalent amounts of protein, which induce responses in which IgM antibody predominates. Using an enzyme-linked immunoassay with isotype-specific conjugates, we found that antibody of all four subclasses of IgG were evoked during the course of the immune responses of C3H/HeAu mice to B. abortus endotoxin. Secondary responses of endotoxin-hyporesponsive C3H/HeJ mice were similar to those seen in C3H/HeAu mice, although lower levels of antibody were produced during their primary responses. The primary responses of BALB/c athymic mice consisted almost entirely of IgG3, and IgGl appeared following a second injection.The effects of lipopolysaccharide (LPS)-associated protein on the immunogenic properties of B. abortus endotoxin were examined by comparing responses to endotoxin with those to a purified B. abortus LPS containing <1% protein. The endotoxin evoked strong primary and secondary responses in which antibody directed to LPS determinants consisted mainly of IgG3 and those to the protein determinants were largely IgGl antibody. Primary and secondary responses to purified LPS consisted mainly of IgG3 antibody. The potential mechanism of the contribution of protein to the immunogenic properties of the endotoxin as well as possible immune mechanisms involved in these responses are discussed. 728 on August 3, 2020 by guest

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Induction of immune and adjuvant immunoglobulin G responses in mice by Brucella lipopolysaccharide

Cited by 27 publications

References 28 publications

Lipopolysaccharide from Brucella abortus behaves as a T-cell-independent type 1 carrier in murine antigen-specific antibody responses

Lipopolysaccharide from Brucella abortus behaves as a T-cell-independent type 1 carrier in murine antigen-specific antibody responses

Alteration of protective and serologic responses in BALB/c mice vaccinated with chemically modified versus nonmodified proteins of Brucella abortus 19

Influence of endotoxin-protein in immunoglobulin G isotype responses of mice to Brucella abortus lipopolysaccharide

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