Induction of immune responses in cattle with a DNA vaccine encoding glycoprotein C of bovine herpesvirus-1

Gupta, Praveen Kumar; Saini, Mohini; Gupta, L. K.; Rao, V. D. P.; Bandyopadhyay, S.; Butchaiah, G.; Garg, G. K.; Garg, Seema

doi:10.1016/s0378-1135(00)00304-7

Cited by 31 publications

(28 citation statements)

References 41 publications

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“…In the present study, we describe the characterisation and the evaluation of several DNA vaccines in cattle in similar conditions. Although these DNA vaccines conferred an immune response comparable to that described by other groups [17,54], they induced weaker immune response and virological protection than the best protocol developed previously with conventional marker vaccine [20]. Therefore, a further experiment will aim at optimising some of the parameters known to modulate the efficacy of DNA vaccines such as the adjuvancy of immunostimulatory CpG motifs in the plasmid backbone [43].…”

Section: Discussionmentioning

confidence: 95%

“…Further DNA vaccination experiments performed in mice and cattle have shown that a construct encoding a secreted form of BoHV-1 gD induces a higher immune response than membrane-associated full-length gD [4,54]. Different routes of DNA administration to cattle have been tested such as intradermic or intramuscular injection [10,17,54] and biolistic delivery (gene-gun) of gold particles coated with DNA vaccines [6,33,34]. Mucosal administration of plasmid DNA via a gene-gun [34] or into suppositories [35] has also been shown to induce systemic as well as mucosal immunity.…”

Section: Introductionmentioning

confidence: 99%

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Genetic immunisation of cattle against Bovine herpesvirus 1: glycoprotein gD confers higher protection than glycoprotein gC or tegument protein VP8

et al. 2005

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-Bovine herpesvirus 1 (BoHV-1) has frequently been used as a model for testing parameters affecting DNA immunisation in large animals like cattle. However, the selection of target antigens has been poorly studied, and most of the experiments have been conducted in mice. In the present study, we demonstrated in cattle that a DNA vaccine encoding BoHV-1 glycoprotein gD induces higher neutralising antibody titres than vaccines encoding BoHV-1 gC. Additionally, we show that a DNA vaccine encoding a secreted form of gD induces a higher immune response than a vaccine encoding full-length gD. However, the enhanced immunogenicity associated with the secretion of gD could not be extended to the glycoprotein gC. The current study also describes for the first time the development and the evaluation of a DNA vaccine encoding the major tegument protein VP8. This construct, which is the first BoHV-1 plasmid vaccine candidate that is not directed against a surface glycoprotein, induced a high BoHV-1 specific cellular immunity but no humoral immune response. The calves vaccinated with the constructs encoding full-length and truncated gD showed a non-significant tenfold reduction of virus excretion after challenge. Those calves also excreted virus for significantly (p < 0.05) shorter periods (1.5 days) than the nonvaccinated controls. The other constructs encoding gC and VP8 antigens induced no virological protection as compared to controls. Altogether the DNA vaccines induced weaker immunity and protection than conventional marker vaccines tested previously, confirming the difficulty to develop efficient DNA vaccines in large species.BoHV-1 / DNA immunisation / gC / gD / VP8

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Section: Discussionmentioning

confidence: 95%

Section: Introductionmentioning

confidence: 99%

Genetic immunisation of cattle against Bovine herpesvirus 1: glycoprotein gD confers higher protection than glycoprotein gC or tegument protein VP8

et al. 2005

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“…The latest vaccine approaches consist of plasmid DNA vaccines containing sequences encoding for the three major immunodominant BoHV-1 glycoproteins gC [64], gB [117] or gD [153,199,200]. These constructs raised the putative vaccination of cattle by needle free delivery methods which would be advantageous to avoid losses due to tissue damage encountered by classical vaccine delivery methods [83,206].…”

Section: New Trends In Bohv-1 Vaccinesmentioning

confidence: 99%

Bovine herpesvirus 1 infection and infectious bovine rhinotracheitis

et al. 2007

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-Bovine herpesvirus 1 (BoHV-1), classified as an alphaherpesvirus, is a major pathogen of cattle. Primary infection is accompanied by various clinical manifestations such as infectious bovine rhinotracheitis, abortion, infectious pustular vulvovaginitis, and systemic infection in neonates. When animals survive, a life-long latent infection is established in nervous sensory ganglia. Several reactivation stimuli can lead to viral re-excretion, which is responsible for the maintenance of BoHV-1 within a cattle herd. This paper focuses on an updated pathogenesis based on a molecular characterization of BoHV-1 and the description of the virus cycle. Special emphasis is accorded to the impact of the latency and reactivation cycle on the epidemiology and the control of BoHV-1. Several European countries have initiated BoHV-1 eradication schemes because of the significant losses incurred by disease and trading restrictions. The vaccines used against BoHV-1 are described in this context where the differentiation of infected from vaccinated animals is of critical importance to achieve BoHV-1 eradication.

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“…Furthermore, herpesvirus gC can bind to the complement component C3b and inhibit the cascade of complement activation [8]. The gC of BHV-1 contains many determinants and can induce strong cytotoxic T cell responses [9][10][11]. However, the nature and function of DEV gC remains unkonwn.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the gene encoding glycoprotein C of duck enteritis virus

Liu

Zhao

et al. 2008

Virus Genes

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A total of 2,718 bp of DNA fragment was amplified from the C-KCE strain of duck enteritis virus (DEV) genome using thermal asymmetric interlaced PCR. This newly identified viral DNA fragment contained two non-overlapping open reading frames (ORFs) oriented from the 5' to 3' direction. The first ORF was comprised of 43.5% G + C and contained the full-length genomic sequence of the UL44 gene (1,296 bp) encoding 431 amino acid residues of DEV glycoprotein C (gC). The second ORF encoded a partial peptide of the UL43 gene. The sequences of DNA and deduced amino acids of the DEV gC gene shared high homology with other members of known herpesviruses, supporting the classification of DEV. Phylogenetic analysis of the DEV gC gene revealed that the gC gene had a close evolutionary relationship with the subfamily of Alphaherpesvirinae.

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Induction of immune responses in cattle with a DNA vaccine encoding glycoprotein C of bovine herpesvirus-1

Cited by 31 publications

References 41 publications

Genetic immunisation of cattle against Bovine herpesvirus 1: glycoprotein gD confers higher protection than glycoprotein gC or tegument protein VP8

Genetic immunisation of cattle against Bovine herpesvirus 1: glycoprotein gD confers higher protection than glycoprotein gC or tegument protein VP8

Bovine herpesvirus 1 infection and infectious bovine rhinotracheitis

Characterization of the gene encoding glycoprotein C of duck enteritis virus

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