Induction of immunostimulatory cytokine genes expression in human PBMCs by a novel semiquinone glucoside derivative (SQGD) isolated from a Bacillus sp. INM-1

Kumar, Raj; Sharma, Rakesh Kumar; Bansal, Deen Dayal; Patel, Dev Dutt; Mishra, Saurabh; Miteva, Lyuba; Dobreva, Zlatka Georgieva; Gadjeva, V.; Stanilova, Spaska

doi:10.1016/j.cellimm.2010.11.005

Cited by 17 publications

(23 citation statements)

References 35 publications

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“…Earlier studies revealed that induction of the pro-survival factor NF-kB led to enhanced G-CSF expression (Chang et al, 1995). Our previous findings also showed that SQGD treatment enhanced Rel A (p65 component of activated NF-kB) expression in human PBMC (Kumar et al, 2011b). Therefore, present findings could help us to establish a direct link between SQGD-mediated G-CSF induction and subsequently NF-kB activation.…”

Section: Effects Of Treatments On M-csf Expression In Bone Marrow Cellsmentioning

confidence: 86%

A semiquinone glucoside derivative isolated fromBacillussp. INM-1 provides protection against 5-fluorouracil-induced immunotoxicity

Mishra

Malhotra

Gupta

et al. 2014

Journal of Immunotoxicology

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Section: Effects Of Treatments On M-csf Expression In Bone Marrow Cellsmentioning

confidence: 86%

A semiquinone glucoside derivative isolated fromBacillussp. INM-1 provides protection against 5-fluorouracil-induced immunotoxicity

Mishra

Malhotra

Gupta

et al. 2014

Journal of Immunotoxicology

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“…Although protein expression of all three subunits will need to be determined when subunit-specific antibodies are available in the future, the rapid induction of p35 by PAMPs suggests that fish macrophages and DCs may be the major producers of IL-12 isoforms early in response to pathogens, as seen in mammals [1,2,35]. Increased p40 expression may not only enhance the secretion of IL-12 heterodimers but also enhance the secretion of p40 itself especially when p35 is limited.…”

Section: Discussionmentioning

confidence: 99%

Differential expression, modulation and bioactivity of distinct fish IL‐12 isoforms: Implication towards the evolution of Th1‐like immune responses

2014

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IL-12 is a heterodimeric cytokine composed of an α-chain (p35) and a β-chain (p40). Primarily produced by APCs, IL-12 induces IFN-γ production in T, B and NK cells. IL-12 drivesTh1-cell differentiation and IFN-γ secretion to promote cell-mediated immunity, which is essential in the defence against intracellular pathogens. The importance of IL-12 in Th1 responses is echoed by its targeted suppression by intracellular pathogens evading cell-mediated immunity. IL-12 subunits have been identified recently in fish, although reported bioactivities are limited to higher vertebrates. Here, we report the cloning of a p35 gene and two divergent p40 genes (p40b and p40c), capable of producing two functional IL-12 isoforms (p35/p40b and p35/p40c) in rainbow trout. Trout IL-12 isoforms possess distinct bioactivities with respect to the induction of IFN-γ, IL-10 and p40c expression. Trout IL-12 isoforms are differentially expressed and modulated in vivo, exhibiting specific gene expression profiles in bacterial, viral and parasitic infection models, and in vitro in stimulated macrophage and leucocyte cultures. These data imply that alternative or additional pathogen-specific Th-like cell populations may exist in fish. This study will facilitate a broader understanding of the evolutionary processes driving host-pathogen interactions and Th1-like immune responses in lower vertebrates.Keywords: Bioactivity r IL-12 r Infection r Rainbow trout r Th1 immune responses Additional supporting information may be found in the online version of this article at the publisher's web-site Introduction IL-12 is a pleiotropic cytokine belonging to the IL-12 family of composite heterodimeric cytokines, with each member consisting of one of three α-chains (p35, p28 and p19) and one of two β-chains (p40 and Ebi3). Structurally related to the IL-6 ligand and receptor families, each chain in mammals is encoded by a single gene [1][2][3][4][5]. Currently, the IL-12 family consists of four cytokine members each with a unique α/β subunit pairing, namely, IL-12 (p35/p40), Correspondence: Dr. Tiehui Wang e-mail: t.h.wang@abdn.ac.uk (p19/p40), IL-27 (p28/Ebi3) and IL-35 (p35/Ebi3), all of which are known to have key roles in the regulation of T-cell responses [2,3,6].Also known as NK-cell stimulatory factor [7] and cytotoxic lymphocyte maturation factor [8], IL-12 is produced primarily by APCs, such as macrophages and DCs, and has been shown to have direct proliferative and cytolytic activities towards pre-activated T cells and NK cells [7][8][9]. IL-12 is a major player in the activation of innate immunity and in the induction of IFN-γ, the cytokine that defines Th1-type immune responses in higher vertebrates. In doing so, IL-12 acts as a crucial molecular switch between innate and adaptive immunity driving Th1 cell differentiation and expansion, whilst inhibiting IL-4 and antagonising Th2 responses [2,3,6,10]. Eur. J. Immunol. 2014Immunol. . 44: 1541Immunol. -1551 Hence, IL-12 is an important prerequisite for the effective clearance of mostly intrace...

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“…Thus, up-regulation of NFKB1 and NFKB2 expression might contribute to the NF-κB-dependent induction of a number of chemokines and cytokines. It has been shown that an increase of transcriptional level of NF-κB was accompanied by the induction of immunostimulatory cytokines in human PBMC and various proteins in cancer cells [24,25].…”

Section: Functions Genesmentioning

confidence: 99%

Gene expression profile of human peripheral blood mononuclear cells induced by Staphylococcus aureus lipoteichoic acid

Kang¹,

Kim²,

Jang³

et al. 2012

International Immunopharmacology

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Induction of immunostimulatory cytokine genes expression in human PBMCs by a novel semiquinone glucoside derivative (SQGD) isolated from a Bacillus sp. INM-1

Cited by 17 publications

References 35 publications

A semiquinone glucoside derivative isolated fromBacillussp. INM-1 provides protection against 5-fluorouracil-induced immunotoxicity

A semiquinone glucoside derivative isolated fromBacillussp. INM-1 provides protection against 5-fluorouracil-induced immunotoxicity

Differential expression, modulation and bioactivity of distinct fish IL‐12 isoforms: Implication towards the evolution of Th1‐like immune responses

Gene expression profile of human peripheral blood mononuclear cells induced by Staphylococcus aureus lipoteichoic acid

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