Induction of increased calcium uptake in mouse T lymphocytes by concanavalin A and its modulation by cyclic nucleotides

Freedman, M. H.; Raff, Martin; Gomperts, Bastien D.

doi:10.1038/255378a0

Cited by 260 publications

(59 citation statements)

References 22 publications

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“…In mouse T lymphocytes, 'gated channels' for Ca", which could be opened directly by Con A binding to membrane glycoproteins, have been postulated, the operation of which may be regulated by cyclic nucleotides (18). From these results, combined with the present data, co-interaction between protein kinase A and protein kinase C in platelets is suggested to be involved in the inhibitory effect of Con A on 5-HT transport.…”

Section: Discussionsupporting

confidence: 60%

Effect of Concanavalin A on Serotonin Transport into Blood Platelets: Possible Involvement of Protein Kinase C

Jikoh

Nishio

Okugawa

et al. 1990

Japanese Journal of Pharmacology

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Section: Discussionsupporting

confidence: 60%

Effect of Concanavalin A on Serotonin Transport into Blood Platelets: Possible Involvement of Protein Kinase C

Jikoh

Nishio

Okugawa

et al. 1990

Japanese Journal of Pharmacology

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“…In addition, the influence of temperature, pH, a metabolic inhibitor, and potential competitive inhibitors was examined. The influence of temperature was examined by comparing antibiotic uptake at 4 and 37°C. The pH profile of CLDM uptake was measured in media that had been preadjusted to different external pH values (from 5.0 to 8.5) by the addition of 10 N HCl or 10 N NaOH.…”

Section: Methodsmentioning

confidence: 99%

High-performance liquid chromatography measurement of antimicrobial concentrations in polymorphonuclear leukocytes

Koga

1987

Antimicrob Agents Chemother

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High-performance liquid chromatography was used to determine the penetration of 19 antimicrobial agents into human polymorphonuclear leukocytes. The ratios of the intracellular concentration to the extraceHlular concentration of ampicillin, piperacillin, cefazolin, ceftizoxime, cefpimizole, and ceftazidime were all less than 0.6. Lincomycin showed a high intracellular-to-extraceliular ratio (3.0), while clindamycin achieved a ratio of 15.5, which was the highest ratio of all of the 19 tested antibiotics. Ratios for rifampin, isoniazid, chloramphenicol, and trimethoprim were 8.2, 1.1, 9.6, and 6.1, respectively. Six quinolone-class antimicrobial agents had ratios from 2.2 to 8.2. Flucytosine showed a penetration ratio of 4.6. Clindamycin uptake was temperature dependent and occurred best with live polymorphonuclear leukocytes; sodium fluoride, adenosine, and puromycin were inhibitory. The results obtained in this study correlate well with the results of other studies involving radioisotopic methods. This indicates that high-performance liquid chromatography is a useful method for determining the intracellular penetration of antimicrobial agents.

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“…Thus, the ACTH action to increase tissue cAMP concentration in both adrenal cortex and adipose tissue is calcium dependent (35)(36)(37). Further, in lymphoid cells in vitro, the mitogen concanavalin A induces calcium-dependent increases in the uptake of both 3-0-MG and glucose (38,39).…”

Section: Effects Of Alprenololmentioning

confidence: 99%

Studies of the mechanism by which 3,5,3'- triiodothyronine stimulates 2-deoxyglucose uptake in rat thymocytes in vitro. Role of calcium and adenosine 3':5'-monophosphate.

Segal¹,

Ingbar²

1981

J. Clin. Invest.

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A B S T R A C T The present experiments were designed to explore the mechanism whereby 3,5,3'-triiodothyronine (T3) stimulates the uptake of 2-deoxy-D-glucose (2-DG) in rat thymocytes in vitro. Addition of T3 evoked a transient, dose-related increase in cellular cyclic (c) AMP concentrations, evident within 5 min, followed soon by an increase in 2-DG uptake. The effects of T3 on both cAMP concentration and 2-DG uptake were dependent upon the presence of extracellular calcium. Epinephrine also induced a sequential increase in thymocyte cAMP concentration and 2-DG uptake. These responses were more prompt than those to T3, but were calcium independent. As with their combined effects on 2-DG uptake, T3 and epinephrine produced synergistic or additive effects on cellular cAMP concentration. Dibutyryl cAMP also stimulated 2-DG uptake, an effect that was more prompt than that of epinephrine, and, like that of epinephrine, was calcium independent.Prior or simultaneous addition of L-alprenolol (10 AM), which, we have previously shown, blocks the effect of both T3 and epinephrine on 2-DG uptake, also blocked the increase in thymocyte cAMP concentration induced by these agents. In contrast, Lalprenolol failed to block the increase in 2-DG uptake produced by dibutyryl cAMP. On the basis of these observations we suggest that T3 increases 2-DG uptake in the rat thymocyte by increasing the cellular concentration of cAMP, which then acts to enhance sugar transport. The increase in 2-DG uptake induced by epinephrine is also mediated by an increase in cAMP concentration. The greater

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Induction of increased calcium uptake in mouse T lymphocytes by concanavalin A and its modulation by cyclic nucleotides

Cited by 260 publications

References 22 publications

Effect of Concanavalin A on Serotonin Transport into Blood Platelets: Possible Involvement of Protein Kinase C

Effect of Concanavalin A on Serotonin Transport into Blood Platelets: Possible Involvement of Protein Kinase C

High-performance liquid chromatography measurement of antimicrobial concentrations in polymorphonuclear leukocytes

Studies of the mechanism by which 3,5,3'- triiodothyronine stimulates 2-deoxyglucose uptake in rat thymocytes in vitro. Role of calcium and adenosine 3':5'-monophosphate.

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