Induction of Interferons (IFNs) and Tumor Necrosis Factor (TNF) in Mice by a Novel Glycolipid Trehalose 2,3,6′‐Trimycolate from <i>Rhodococcus aurantiacus (Gordona aurantiaca)</i>

Fujita, Takako; Sugimoto, Nobuko; Yokoi, Fusa; Ohtsubo, Yoshikazu; Ikutoh, Masatoshi; Kato, Yoshiko; Natsuhara, Y.; Oka, Shiro; Yano, Ikuya

doi:10.1111/j.1348-0421.1990.tb03169.x

Cited by 8 publications

(4 citation statements)

References 27 publications

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“…(4,10,24) Not surprisngly, TNF-a protein was detected in the supernatants from lung minces obtained 5 days after injection of TDM emulsions. The TNF-a response varied greatly, approximately 20-fold, among mice treated with the glycolipid (range 48-1168 pg/ml, mean 416 pg/ml, median 332 pg/ml).…”

Section: Relationship Of Lung Tnf-a Expression To Inflammatory Intensmentioning

confidence: 99%

Cytokine Message and Protein Expression During Lung Granuloma Formation and Resolution Induced by the Mycobacterial Cord Factor Trehalose-6,6′-Dimycolate

Perez

Roman

Roser

et al. 2000

Journal of Interferon & Cytokine Research

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Trehalose-6,6'-dimycolate (TDM), or cord factor, is a mycobacterial cell wall component that induces granuloma formation and proinflammatory cytokine production in vivo and in vitro. The purpose of this work was to better understand the mechanisms by which TDM promotes lung granuloma formation. This was accomplished by characterizing cytokine mRNA expression during TDM-induced alveolitis culminating in cohesive granuloma development. A single intravenous injection of TDM given to C57BL/6 mice produced lung granulomas that peaked in number 5 days after challenge and were nearly resolved by 14 days. mRNA in whole lung preparations was quantitated by bioluminescent RT-PCR. Tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), and IL-6 were significantly elevated during granuloma development and decreased during granuloma resolution. There were no detectable changes in mRNA for interferon-y (IFN-y), IL-2, IL-4, IL-5, IL-10, and IL-12(p40). The level of TNF-alpha protein extracted from lung minces highly correlated with morphologic indices of granulomatous inflammation, indicating that it may be an important modulator of the inflammatory intensity induced by TDM. TDM may interact specifically with macrophages in vivo, as evidenced by induction of TNF-alpha, IL-1beta, and IL-6, but not IFN-gamma, protein in bone marrow-derived macrophages from C57BL/6 mice. TDM may therefore play an important role early in macrophage activation during the host granulomatous response to mycobacteria.

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Section: Relationship Of Lung Tnf-a Expression To Inflammatory Intensmentioning

confidence: 99%

Cytokine Message and Protein Expression During Lung Granuloma Formation and Resolution Induced by the Mycobacterial Cord Factor Trehalose-6,6′-Dimycolate

Perez

Roman

Roser

et al. 2000

Journal of Interferon & Cytokine Research

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“…It has been widely assumed that cord factor is directly responsible for the wasting associated with mycobacterial infections. Recently, cord factor has also been found to be a potent immunomodulator, possessing granuloma-forming activity and antitumor activity (7,18,28).…”

Section: Pectedmentioning

confidence: 99%

Increase of T-cell receptor gamma/delta-bearing T cells in cord blood of newborn babies obtained by in vitro stimulation with mycobacterial cord factor

et al. 1991

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Cord blood T lymphocytes proliferated in vitro in response to mycobacterial organisms but did not proliferate in the presence of tuberculin purified protein derivative. Components recognized by cord blood T cells were resistant to protease digestion. In contrast, T lymphocytes derived from tuberculin-positive adult peripheral blood proliferated when stimulated by the protease-sensitive component of mycobacterial organisms or purified protein derivative, confirming that adult T cells respond to protein components whereas cord blood T cells respond to the nonpeptide component of mycobacteria. In vitro culture of cord blood lymphocytes stimulated by either mycobacterial lysates or the lipid fraction showed increases in the numbers of T-cell receptor (TcR)-y/6 T lymphocytes with no changes in the numbers of TcR aLlp T lymphocytes in contrast to the

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“…This corresponds to the data by Schelhaas [5], who identified Glc, Man and Ara by gas chromatography. Immunostimulating activity was also found in other Rhodococcus species [15][16][17]. These reports differ not only in the species but relate to either glcyolipids [15] or whole cells [16,17].…”

Section: Discussionmentioning

confidence: 96%

Detection, isolation and partial characterization of an immunostimulating glycoprotein from Rhodococcus fascians

Butschak

Karsten

Schelhaas

et al. 2006

International Immunopharmacology

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Induction of Interferons (IFNs) and Tumor Necrosis Factor (TNF) in Mice by a Novel Glycolipid Trehalose 2,3,6′‐Trimycolate from Rhodococcus aurantiacus (Gordona aurantiaca)

Abstract: The immunomodifying activity of a novel mycoloyl glycolipid, trehalose 2,3,6'-trimycolate (GaGM), from a unique psychrophilic acid-fast bacterium.

Cited by 8 publications

References 27 publications

Cytokine Message and Protein Expression During Lung Granuloma Formation and Resolution Induced by the Mycobacterial Cord Factor Trehalose-6,6′-Dimycolate

Cytokine Message and Protein Expression During Lung Granuloma Formation and Resolution Induced by the Mycobacterial Cord Factor Trehalose-6,6′-Dimycolate

Increase of T-cell receptor gamma/delta-bearing T cells in cord blood of newborn babies obtained by in vitro stimulation with mycobacterial cord factor

Detection, isolation and partial characterization of an immunostimulating glycoprotein from Rhodococcus fascians

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