Induction of keratinocyte apoptosis by photosensitizing chemicals plus UVA

Kurita, Masako; Shimauchi, Takatoshi; Miwa, Koichi; Atarashi, Kenji; Mori, Koji; Tokura, Y.

doi:10.1016/j.jdermsci.2006.10.010

Cited by 30 publications

(22 citation statements)

References 30 publications

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“…The procedure involves exposing the cornea to 30 mW of UV-A irradiation for 3 min in the presence of riboflavin instead of the regular protocol of 3 mW for 30 min, with the total energy delivered being the same in both methods 30. The adverse and cytotoxic effects of high UV-A irradiation has been well documented on skin epithelium or keratinocytes 31. Though there have been studies on the effect of ACXL on animal eyes, there have been none on human LECs 11 32 33.…”

Section: Discussionmentioning

confidence: 99%

Safety profile of accelerated corneal cross-linking versus conventional cross-linking: a comparative study on ex vivo-cultured limbal epithelial cells

et al. 2014

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Section: Discussionmentioning

confidence: 99%

Safety profile of accelerated corneal cross-linking versus conventional cross-linking: a comparative study on ex vivo-cultured limbal epithelial cells

et al. 2014

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“…More recent causative agents include cosmetic or sunscreen products such as 6-methylcoumarin, musk ambrette, benzophenone. Likewise, there have been various culprit drugs to evoke photosensitivity, including chlorpromazine, promethazine, quinolones, such as sparfloxacin and enoxacin, piroxicum, afloqualone, and non-steroidal anti-inflammatory drugs (Kurita et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

“…For phototoxicity testing, the in vitro 3T3 NRU phototoxicity test has been adopted as an alternative to in vivo phototoxicity test (OECD n°432). In vitro replacements for the in vivo photoallergy test have also been sought (Tokura, 2000;Lovell and Jones, 2000;Barratt et al, 2000;Neumann et al, 2005;Onoue and Tsuda, 2005;Kurita et al, 2007, Hoya et al, 2009Karschk et al, 2010), but to date no accepted alternative is available to identify the photoallergenic potential of new chemicals.…”

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confidence: 99%

Establishment of an in vitro photoallergy test using NCTC2544 cells and IL-18 production

Galbiati

Martı́nez

Bianchi

et al. 2013

Toxicology in Vitro

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Differentiation between photoallergenic and phototoxic reactions induced by low molecular weight compounds represents a current problem. The use of keratinocytes as a potential tool for the detection of photoallergens as opposed to photoirritants is considered an interesting strategy for developing in vitro methods. We have previously demonstrated the possibility to use the human keratinocyte cell line NCTC2455 and the production of interleukin-18 (IL-18) to screen low molecular weight sensitizers. The purpose of this work was to explore the possibility to use the NCTC2544 assay to identify photoallergens and discriminate from phototoxic chemicals.First, we identified suitable condition of UV-irradiation (3.5 J/cm 2 ) by investigating the effect of UVAirradiation on intracellular IL-18 on untreated or chloropromazine (a representative phototoxic compound)-treated NCTC2544 cells. Then, the effect of UVA-irradiation over NCTC2544 cells treated with increasing concentrations of 15 compounds including photoallergens (benzophenone, 4-ter-butyl-4-methoxydibenzoylmethane, 2-ethylexyl-p-methoxycinnamate, ketoprofen, 6-methylcumarin); photoirritant and photoallergen (4-aminobenzoic acid, chlorpromazine, promethazine); photoirritants (acridine, ibuprofen, 8-methoxypsoralen, retinoic acid); and negative compounds (lactic acid, SDS and p-phenilendiamine) was investigated. Twenty-four hours after exposure, cytotoxicity was evaluated by the MTT assay or LDH leakage, while ELISA was used to measure the production of IL-18. At the maximal concentration assayed with non-cytotoxic effects (CV80 under irradiated condition), all tested photoallergens induced a significant and a dose-dependent increase of intracellular IL-18 following UVA irratiation, whereas photoirritants failed.We suggest that this system may be useful for the in vitro evaluation of the photoallergic potential of chemicals.

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“…The ROS generated by UVA radiation include singlet oxygen, superoxide, hydrogen peroxide and hydroxyl radical [14–16]. It is well established that these ROS not only exert oxidative damage, but may also play a central role in oxidative stress signaling pathways leading to cellular damage/apoptosis or adaptive responses [17–19]. Therefore, it may be thought that the antioxidant cell status could be critical in regulating cell behavior [20].…”

Section: Introductionmentioning

confidence: 99%

UVA Irradiation of Dysplastic Keratinocytes: Oxidative Damage versus Antioxidant Defense

Nechifor

Niculite

Urs

et al. 2012

IJMS

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UVA affects epidermal cell physiology in a complex manner, but the harmful effects have been studied mainly in terms of DNA damage, mutagenesis and carcinogenesis. We investigated UVA effects on membrane integrity and antioxidant defense of dysplastic keratinocytes after one and two hours of irradiation, both immediately after exposure, and 24 h post-irradiation. To determine the UVA oxidative stress on cell membrane, lipid peroxidation was correlated with changes in fatty acid levels. Membrane permeability and integrity were assessed by propidium iodide staining and lactate dehydrogenase release. The effects on keratinocyte antioxidant protection were investigated in terms of catalase activity and expression. Lipid peroxidation increased in an exposure time-dependent manner. UVA exposure decreased the level of polyunsaturated fatty acids, which gradually returned to its initial value. Lactate dehydrogenase release showed a dramatic loss in membrane integrity after 2 h minimum of exposure. The cell ability to restore membrane permeability was noted at 24 h post-irradiation (for one hour exposure). Catalase activity decreased in an exposure time-dependent manner. UVA-irradiated dysplastic keratinocytes developed mechanisms leading to cell protection and survival, following a non-lethal exposure. The surviving cells gained an increased resistance to apoptosis, suggesting that their pre-malignant status harbors an abnormal ability to control their fate.

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Induction of keratinocyte apoptosis by photosensitizing chemicals plus UVA

Cited by 30 publications

References 30 publications

Safety profile of accelerated corneal cross-linking versus conventional cross-linking: a comparative study on ex vivo-cultured limbal epithelial cells

Safety profile of accelerated corneal cross-linking versus conventional cross-linking: a comparative study on ex vivo-cultured limbal epithelial cells

Establishment of an in vitro photoallergy test using NCTC2544 cells and IL-18 production

UVA Irradiation of Dysplastic Keratinocytes: Oxidative Damage versus Antioxidant Defense

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