Induction of Laccase, Lignin Peroxidase and Manganese Peroxidase Activities in White-Rot Fungi Using Copper Complexes

Vršanská, Martina; Voběrková, Stanislava; Langer, Vratislav; Palovčíková, D.; Moulick, Amitava; Adam, Vojtěch; Kopel, Pavel

doi:10.3390/molecules21111553

Cited by 72 publications

(30 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In addition to ligninolytic enzymes, certain whiterot fungi also produce cellulose-degrading enzymes (β-glucosidase, cellobiohydrolase, and β-xylosidase; Vrsanska et al, 2016), resulting in simultaneous degradation of lignin and cellulose components by several strains (Trametes versicolor, Heterobasidium annosum, and Irpex lacteus). Consequently, white-rot fungi can improve digestibility and nutritive value of low-quality forages such as wheat straw and bermudagrass (Akin et al, 1993;Nayan et al, 2018).…”

Section: White-and Brown-rot Fungimentioning

confidence: 99%

Symposium review: Technologies for improving fiber utilization

Adesogan

Arriola

Jiang

et al. 2019

Journal of Dairy Science

107

View full text Add to dashboard Cite

The forage lignocellulosic complex is one of the greatest limitations to utilization of the nutrients and energy in fiber. Consequently, several technologies have been developed to increase forage fiber utilization by dairy cows. Physical or mechanical processing techniques reduce forage particle size and gut fill and thereby increase intake. Such techniques increase the surface area for microbial colonization and may increase fiber utilization. Genetic technologies such as brown midrib mutants (BMR) with less lignin have been among the most repeatable and practical strategies to increase fiber utilization. Newer BMR corn hybrids are better yielding than the early hybrids and recent brachytic dwarf BMR sorghum hybrids avoid lodging problems of early hybrids. Several alkalis have been effective at increasing fiber digestibility. Among these, ammoniation has the added benefit of increasing the nitrogen concentration of the forage. However, few of these have been widely adopted due to the cost and the caustic nature of the chemicals. Urea treatment is more benign but requires sufficient urease and moisture for efficacy. Ammonia-fiber expansion technology uses high temperature, moisture, and pressure to degrade lignocellulose to a greater extent than ammoniation alone, but it occurs in reactors and is therefore not currently usable on farms. Biological technologies for increasing fiber utilization such as application of exogenous fibrolytic enzymes, live yeasts, and yeast culture have had equivocal effects on forage fiber digestion in individual studies, but recent meta-analyses indicate that their overall effects are positive. Nonhydrolytic expansinlike proteins act in synergy with fibrolytic enzymes to increase fiber digestion beyond that achieved by the enzyme alone due to their ability to expand cellulose microfibrils allowing greater enzyme penetration of the cell wall matrix. White-rot fungi are perhaps the biological agents with the greatest potential for lignocellulose deconstruction, but they require aerobic conditions and several strains degrade easily digestible carbohydrates. Less ruminant nutrition research has been conducted on brown rot fungi that deconstruct lignocellulose by generating highly destructive hydroxyl radicals via the Fenton reaction. More research is needed to increase the repeatability, efficacy, cost effectiveness, and onfarm applicability of technologies for increasing fiber utilization.

show abstract

Section: White-and Brown-rot Fungimentioning

confidence: 99%

Symposium review: Technologies for improving fiber utilization

Adesogan

Arriola

Jiang

et al. 2019

Journal of Dairy Science

107

View full text Add to dashboard Cite

show abstract

“…Ligninases and cellulases are important extracellular C-acquiring enzymes involved in litter decomposition. The lignin decomposition system consists of three principle ligninases: laccase, manganese peroxidase (MnP) and lignin peroxidase (LiP) 13 . Cellulose-degrading microorganisms secrete numerous cellulolytic enzymes (mainly cellobiohydrolase, endo-1,4-β-D-glucanase (EG) and β-glucosidase (BG)), which act synergistically to completely degrade lignocellulosic biomass 14,15 .…”

Section: Introductionmentioning

confidence: 99%

Litter quality drives the differentiation of microbial communities in the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau

Zheng

Chen

Liu

et al. 2018

Sci Rep

View full text Add to dashboard Cite

Cellulose and lignin are the main polymeric components of the forest litter horizon. We monitored microbial community composition using phospholipid fatty acid (PLFA) analysis and investigated the ligninolytic and cellulolytic enzyme activities of the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau. The activities of ligninolytic and cellulolytic enzymes and the biomass of microbial PLFAs were higher in the initial stage of litter decomposition than in the latter stage in the three vegetation types (coniferous forest, alpine shrubland and alpine meadow). Soil microbial community structure varied significantly over the course of litter decomposition in the three vegetation types. Furthermore, the BIOENV procedure revealed that the carbon to nitrogen (C:N) ratio, carbon to phosphorus (C:P) ratio and moisture content (MC) were the most important determinants of microbial community structure in the initial stage of litter decomposition, whereas pH and the lignin concentration were the major factors influencing the microbial community structure in the later stage of litter decomposition. These findings indicate that litter quality drives the differentiation of microbial communities in the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau.

show abstract

“…It is well known that white-rot fungus secrete extracellular enzymes (mainly laccase, manganese peroxidase, lignin peroxidase) to degrade lignin. These enzymes all need the transport of metal ions, Cu, Zn, Fe, Mn [25,26]. Also, coenzymes are a kind of compounds with special chemical structure and function, which widely participate in enzyme-catalyzed redox reactions and undertake the function of transferring electrons, atoms or groups in enzyme-catalyzed reactions, for example, NAD + or NADP + carry reducing hydrogen.…”

Section: Differential Gene Expression As a Function Of Wood Stimulusmentioning

confidence: 99%

Transcriptomics analysis and co-expression network revealing candidate genes for laccase activity of Lenzites gibbosa

Chen¹,

Chi²,

Zhao³

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Lenzites gibbosa is a common white-rot fungus of Polyporaceae in the cold temperate zone which cause spongy white decay of wood. Results In this study, the lignin degradation pathway of L. gibbosa at 5-time points under wood treatment was studied by RNA-Req technology. A total of 5232 DEGs were identified from 15 libraries. In 0–3 days, the mycelia are in the adaptive stage, and the mycelia began to proliferate within 3–5 days. After 5 days, the number of DGEs decreased significantly, the mycelium growth entered the platform stage, and the life activity was basically stable. In the secondary metabolism, oxidoreductase such as laccase, 2-oxoglutarate-Fe (II) type oxidoreductase, peroxisomal hydratase-dehydrogenase, dual-functional monooxygenase dominated and increased steadily, and manganese peroxidase appeared in the middle stage. With the accumulation of lignin intermediate products, P450 and ABC transporters were from the inhibition to the activation. Weighted gene co-expression network analysis (WGCNA) showed that among the 24 modules 6 modules were significantly correlated with laccase activity and the most correlated were turquoise and blue module. The central hub genes were also identified, including gene_7458, gene_61, gene_7458, gene_1741, gene_11087 which were consistent with the DGEs. These genes have high connectivity, module membership, and gene significance in the module. The enrichment analysis of GO and KEGG pathway indicated that the genes involved in cell cycle, citrate cycle (TCA cycle), nicotinate and nicotinamide metabolism, mitochondrial outer membrane, succinate dehydrogenase, carboxypeptidase and exopeptidase activity, flavin adenine dinucleotide binding, oxidoreductase activity, acting on the CH-CH group of donors, quinone or related compound as acceptor pathway were highly related to laccase synthesis pathway. Construction of gene co-expression network and hypothetical L. gibbosa laccase synthesis pathway. Conclusion This study focused on the screening of L. gibbosa degradation of lignin-related genes. And, this is the first study reporting co-expression patterns of a gene network in L. gibbosa laccase activity after wood treatment which is helpful to understand the synthesis pathway of laccase and improve the activity of laccase, which can be used to increase the rate of lignin degradation by L. gibbosa in the further.

show abstract

Induction of Laccase, Lignin Peroxidase and Manganese Peroxidase Activities in White-Rot Fungi Using Copper Complexes

Cited by 72 publications

References 44 publications

Symposium review: Technologies for improving fiber utilization

Symposium review: Technologies for improving fiber utilization

Litter quality drives the differentiation of microbial communities in the litter horizon across an alpine treeline ecotone in the eastern Tibetan Plateau

Transcriptomics analysis and co-expression network revealing candidate genes for laccase activity of Lenzites gibbosa

Contact Info

Product

Resources

About