Induction of Long-Term Memory CD8<sup>+</sup>T Cells for Recall of Viral Clearing Responses against Influenza Virus

Deliyannis, Georgia; Jackson, David C.; Ede, Nicholas J.; Zeng, Weiguang; Hourdakis, Irene; Sakabetis, Evaline; Brown, Lorena E.

doi:10.1128/jvi.76.9.4212-4221.2002

Cited by 75 publications

(76 citation statements)

References 52 publications

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“…The peptide epitopes that form the core of these vaccines do not elicit any anti-viral antibodies and we have previously determined by depletion studies that the CD4 + T cells induced in response to lipopeptide vaccines do not participate in viral clearance [9]. The pulmonary protection we observe is therefore largely, if not completely, dependent upon the activity of CD8 + T cells directed towards the highly conserved epitope within the nucleoprotein.…”

Section: Resultsmentioning

confidence: 73%

Intranasal lipopeptide primes lung‐resident memory CD8⁺ T cells for long‐term pulmonary protection against influenza

et al. 2006

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The longevity of the influenza virus-specific CD8 + T cell response following intranasal delivery of a synthetic lipopeptide was investigated and the characteristics and location of the cells associated with viral clearance examined. The lipopeptide, incorporating an epitope for CD8 + T cells and another for CD4 + T cells with the lipid moiety S-[2,3-bis(palmitoyloxy)propyl]cysteine (Pam2Cys) attached, induced potent and long-lived pulmonary protection. Both the lipopeptide and its largely unprotective non-lipidated counterpart elicited comparable numbers of CD8 + T cells in the spleen, which was the main location of the memory pool. However, the lipopeptide, unlike the non-lipidated peptide, also induced a substantial memory population that remained in the lungs and was rapidly activated upon viral challenge months later. These lipopeptide-induced lung-resident CD8 + T cells were also very similar in number and IFN-c-secreting potential to those induced by prior exposure to the virus itself and are likely mediators of initial viral clearance prior to recruitment from the expanding lymph node T cell pool. Significant clearing responses were demonstrated as late as 9 months post-lipopeptide vaccination. This study shows that CD8 + T cells primed by the lipopeptide are not only long-lived but can take up residence in the lung where they are important early mediators of pulmonary protection.

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Section: Resultsmentioning

confidence: 73%

Intranasal lipopeptide primes lung‐resident memory CD8⁺ T cells for long‐term pulmonary protection against influenza

et al. 2006

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“…We noticed that CMI responses to HEV in the convalescent chimpanzees were still detectable 3-4 years after infection despite the absence of chronicity in HEV infection. Previous reports support the presence of long-lived CMI responses after viral infection (Deliyannis et al, 2002;Effros, 2000;Van Epps et al, 2002) and vaccination (Hammarlund et al, 2003). Addition of IL-7 and IL-15, cytokines which specifically stimulate memory responses (Guimond et al, 2005;Jennes et al, 2002), significantly increased the frequency of Ag-specific T cells in these convalescent chimpanzees.…”

Section: Discussionmentioning

confidence: 90%

Characterization of hepatitis E-specific cell-mediated immune response using IFN-γ ELISPOT assay

Shata

Barrett

Shire

et al. 2007

Journal of Immunological Methods

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In developing countries, hepatitis E (HEV) and hepatitis A (HAV) are the major causes of acute viral hepatitis with similar fecooral modes of transmission. In contrast to the high seroprevalence of hepatitis A infection, a low seroprevalence of HEV among children in endemic areas has been reported. These data suggest the possibility that silent HEV infection is undiagnosed by the current available methods. Many of the serological tests used for HEV diagnosis have poor specificity and are unable to differentiate among different genotypes of HEV. Moreover, the RT-PCR used for HEV isolation is only valid for a brief period during the acute stage of infection. Cell-mediated immune (CMI) responses are highly sensitive, and long lasting after sub-clinical infections as shown in HCV and HIV. Our objective was to develop a quantitative assay for cell-mediated immune (CMI) responses in HEV infection as a surrogate marker for HEV exposure in silent infection. Quantitative assessment of the CMI responses in HEV will also help us to evaluate the role of CMI in HEV morbidity. In this study, an HEV-specific interferon-gamma (IFN-γ) ELISPOT assay was optimized to analyze HEV-specific CMI responses. We used peripheral blood mononuclear cells (PBMC) and sera from experimentally infected chimpanzees and from seroconverted and control human subjects to validate the assay. The HEV-specific IFN-γ ELISPOT responses correlated strongly and significantly with anti-HEV ELISA positive/negative results (rho=0.73, p=0.02). Moreover, fine specificities of HEV-specific T cell responses could be identified using overlapping HEV ORF2 peptides.

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“…12 The infectivity of the virus stock was determined by plaque formation in MDCK cells 12 Influenza virus-specific T cells are raised in culture using viruspulsed syngeneic antigen-presenting cells (APC). During their generation, antitumor activity is conferred on them by transduction with a retroviral vector encoding a chimeric anti-erbB2 receptor.…”

Section: Virus and Peptidesmentioning

confidence: 99%

Antitumor activity of dual-specific T cells and influenza virus

et al. 2007

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Induction of Long-Term Memory CD8⁺T Cells for Recall of Viral Clearing Responses against Influenza Virus

Abstract: Induction of cytotoxic T-cell-mediated virus-clearing responses by influenza virus T cell determinant-con-

Cited by 75 publications

References 52 publications

Intranasal lipopeptide primes lung‐resident memory CD8⁺ T cells for long‐term pulmonary protection against influenza

Intranasal lipopeptide primes lung‐resident memory CD8⁺ T cells for long‐term pulmonary protection against influenza

Characterization of hepatitis E-specific cell-mediated immune response using IFN-γ ELISPOT assay

Antitumor activity of dual-specific T cells and influenza virus

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Induction of Long-Term Memory CD8+T Cells for Recall of Viral Clearing Responses against Influenza Virus

Abstract: Induction of cytotoxic T-cell-mediated virus-clearing responses by influenza virus T cell determinant-con-

Cited by 75 publications

References 52 publications

Intranasal lipopeptide primes lung‐resident memory CD8+ T cells for long‐term pulmonary protection against influenza

Intranasal lipopeptide primes lung‐resident memory CD8+ T cells for long‐term pulmonary protection against influenza

Characterization of hepatitis E-specific cell-mediated immune response using IFN-γ ELISPOT assay

Antitumor activity of dual-specific T cells and influenza virus

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Product

Resources

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Induction of Long-Term Memory CD8⁺T Cells for Recall of Viral Clearing Responses against Influenza Virus

Intranasal lipopeptide primes lung‐resident memory CD8⁺ T cells for long‐term pulmonary protection against influenza

Intranasal lipopeptide primes lung‐resident memory CD8⁺ T cells for long‐term pulmonary protection against influenza