Induction of macrophage DNA synthesis <i>In vitro</i>, by non‐immunological inflammatory sera: Effect of irradiation and thymus or bone marrow cell reconstitution

Pelletier, Monique; Girre, Catherine; Giroud, J. P.

doi:10.1002/path.1711310208

Cited by 7 publications

(2 citation statements)

References 9 publications

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“…However, since our fraction has a molecular weight between 50(02000 it may be assumed that it is not either a monokine or lymphokine. The existence of a mitogenic factor for macrophages has been demonstrated in the serum of rats having undergone an acute nonspecific inflammatory reaction [15] which has similar molecular weight characteristics to the factor described here [16]. This factor is thought to be of~a peptidic nature since the mitogenic activity for macrophages can be destroyed by prior incubation with trypsin or pronase (unpublished observations).…”

Section: Influence Of Fractionated Inflammatory Serum On 100 the CL Rmentioning

confidence: 57%

Modification of the chemiluminescent response of rat thymocytes to concanavalin A by the acute non-specific inflammatory process

et al. 1984

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Using the technique of concanavalin A-induced chemiluminescence (CL) as a measure of lymphocyte reactivity it has been demonstrated that the acute non-specific inflammatory process initiated by a non-diffusible, non-antigenic, endotoxin free irritant, was able to enhance the CL response of rat thymocytes. Maximum enhancement was observed 48 h after initiation of the inflammatory reaction. Serum derived from animals undergoing an acute inflammatory reaction as used above was fractionated using the technique of ultrafiltration to yield a fraction of molecular weight range 500-2000 daltons. When this fraction was cultured in vitro for 22 h with normal thymocytes, the CL response to concanavalin A was greatly enhanced when compared to thymocytes cultured in the presence of an equivalent fraction of normal serum. These findings demonstrate that during an acute non-immunological inflammatory reaction the function of the hosts thymocytes is enhanced. Furthermore, a low molecular weight factor (500-2000), with thymocyte stimulating activity, may be recovered from the serum of such animals very quickly (within 2 h) after initiation of the acute inflammation.

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Section: Influence Of Fractionated Inflammatory Serum On 100 the CL Rmentioning

confidence: 57%

Modification of the chemiluminescent response of rat thymocytes to concanavalin A by the acute non-specific inflammatory process

et al. 1984

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“…Additional studies using inflammatory exudate from calcium pyrophosphate injected rats demonstrated that the exudate, when cultured with spleen cells, was able to modulate their response to the mitogen phytohaemagglutinin (PHA) [3]. It was suggested that the mitogenie activity of inflammatory serum could be related to both thymic and bone marrow cells and that this cellular activity is enhanced when inflammation is present [5]. For example, sera obtained from such animals reduced the chemotactic responsiveness of polymorphonuclear leukocytes [4].…”

Section: Introductionmentioning

confidence: 99%

Anin vitro effect of an acute non-specific inflammatory serum on rat lymphocytes proliferative response to PHA

et al. 1984

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This study has demonstrated that serum obtained from animals undergoing an acute inflammatory reaction induced by an intrapleural injection of dextran is able to modulate the proliferative response to PHA of lymph node and spleen cells in vitro. This response is dependent on the concentration of the inflammatory serum and on the time of collection of the serum during the acute inflammatory process. At low concentrations of serum (0.5%) stimulatory activity was observed at all time points. At higher concentrations (1%) inhibitory activity was present in 24 and 72 h sera. These results support the previous observation that the acute non-specific inflammatory reaction is able to modify the function of different cell types. It has been suggested that both stimulatory and inhibitory factors are present and the balance between these changes during the course of the inflammatory reaction.

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Modification of thymo-dependent and thymo-independent antibody responses during non-specific acute inflammation

Florentin¹,

Pelletier²,

Giroud³

1984

International Journal of Immunopharmacology

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Induction of macrophage DNA synthesis In vitro, by non‐immunological inflammatory sera: Effect of irradiation and thymus or bone marrow cell reconstitution

Cited by 7 publications

References 9 publications

Modification of the chemiluminescent response of rat thymocytes to concanavalin A by the acute non-specific inflammatory process

Modification of the chemiluminescent response of rat thymocytes to concanavalin A by the acute non-specific inflammatory process

Anin vitro effect of an acute non-specific inflammatory serum on rat lymphocytes proliferative response to PHA

Modification of thymo-dependent and thymo-independent antibody responses during non-specific acute inflammation

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