Induction of mast cell proliferation, maturation, and heparin synthesis by the rat c-kit ligand, stem cell factor.

Abstract

Induction of mast cell proliferation, maturation, and heparin synthesis by the rat c-kit ligand, stem cell factor (developmental ABSTRACTWe investigated the effects of a newly recognized multifunctional growth factor, the c-kit ligand stem cell factor (SCF), on mouse mast cell proliferation and phenotype.Recombinant rat SCF'64 (rrSCF"64) induced the development of large numbers of dermal mast cells in normal mice in vivo.Many of these mast cells had features of "connective tissue-type mast cells" (CTMC), in that they were reactive both with the heparin-binding fluorescent dye berberine sulfate and with safranin. In vitro, rrSCFI64 induced the proliferation of cloned interleukin 3 (IL-3)-dependent mouse mast cells and primary populations of IL-3-dependent, bone marrow-derived cultured mast cells (BMCMC), which represent immature mast cells, and purified peritoneal mast cells, which represent a type of mature CTMC. BMCMC maintained in rrSCF164 not only proliferated but also matured. Prior to exposure to rrSCF'"4,

Inhibition of mast cells by interleukin‐10 gene transfer contributes to protection against acute myocarditis in rats

“…The concentration of c-kit ligand or SCF, which is known to induce differentiation, aggregation, growth and activation of mast cells [37][38][39][40][41], was reduced by IL-10 gene transfer when compared to that in the acute myocarditis group, as seen in the immunohistochemical staining. Therefore, IL-10 gene transfer may have prevented mast cell degranulation and decreased the growth and density of mast cells via these mechanisms.…”

Differential roles of GATA‐1 and GATA‐2 in growth and differentiation of mast cells

“…We found that the expression of GATA-1 was induced in BMMCs by two cytokines, IL-3 and SCF, which are known to induce growth and differentiation of BMMCs (Rennick et al 1995). SCF has been shown to induce BMMCs to acquire the characteristics of CTMCs which have high histamine and heparin content (Tsai et al 1991). In addition, GATA-1 is detected strongly in the nuclei of PMCs, which are another subtype of CTMCs.…”

Specific and common activities of the FLT3 and KIT tyrosine kinase receptors revealed by the use of cultured mast cells

“…20,21,55 Maturation is dependent on a functional KIT receptor; 56 alternatively it is also mimicked by incubation of the mast cells with purified KL, but only upon IL3 withdrawal. 25 The maturation process can be monitored by the stage specific expression of mast cell proteases (MCP) from an immature to a more mature phenotype: the carboxypeptidase A protease (MC-CPA), the murine mast cell protease 6 (mMCP6), and the mMCP4 protease.…”

“…18 In hematopoietic differentiation, W mutants show defects in very early multilineage progenitors, early and late erythropoiesis, Correspondence: P Dubreuil; Fax: 33 491 260 364 Received 12 January 1997; accepted 3 January 1998 as well as in the development and function of mast cells. 18,19 Activation of KIT in normal mast cells stimulates different responses, their proliferation, [20][21][22] survival, 23 and/or differentiation. 20,21,24,25 KIT also participates in mast cell adhesion to extracellular matrix laid down by fibroblasts.…”

“…18,19 Activation of KIT in normal mast cells stimulates different responses, their proliferation, [20][21][22] survival, 23 and/or differentiation. 20,21,24,25 KIT also participates in mast cell adhesion to extracellular matrix laid down by fibroblasts. [26][27][28][29] The synergistic activities exerted by FLT3 and KIT on a small fraction of cycling HSC suggest that a common transduction pathway exists at an early stage of HSC differentiation, and which results in the expansion of the specific committed progenitor population.…”