Induction of micronucleus and nuclear alterations in fish (Oreochromis niloticus) by a petroleum refinery effluent

Inducción de micronúcleos y otras anormalidades nucleares en Astyanax gr. bimaculatus (Pisces: Characidae) expuestas a fenantreno

“…Entre los efectos no controlables de la inmersión pueden citarse las pérdidas por volatilización, absorción y metabolismo (Prosser et al, 2011). A este respecto, en O. mossambicus se evaluó la respuesta a la exposición a PHE usando la vía de administración IP (1 µg/g PV) e inmersión (1 mg/L); aunque ambas exposiciones indujeron la formación de ciertos tipos de aductos de ADN (etapas primarias de procesos mutagénicos) en bilis, la inyección IP indujo los mayores efectos (Wahidulla y Rajamanickam, 2010 efectos mutagénicos de los HAPs se ha evaluado bajo condiciones controladas de laboratorio registrando la presencia de MN y AN, observándose una relación dosis-respuesta (Baršienè et al, 2006;Hoshina et al, 2008;Vanzella et al, 2007 (Costa et al, 2011;Souza y Fontanetti, 2006 (Fig. 3B).…”

<b>Influence of natural radon and metal contamination on surface water quality from a Brazilian Semiarid Region

“…Mutagenic assays have been very useful tools for detecting genetic damage caused by exposure to xenobiotics in plants, animals and humans (Garcia et al, 2011;Hoshina et al, 2008;Fenech et al, 2011).…”

“…Mutagenicity tests have been applied to evaluate different pollutants and contaminants in freshwater ecosystems (Misík, et al, 2011;Vincent-Huber, Heas-Moisan, & Munschy, 2012). Micronuclei (MN) test is one of the most used assays in different models to determine chromosomal damage caused by the presence of compounds and mixture of substances (Misík, et al, 2011;Garcia et al, 2011;Hoshina, Angelis, & Marin-Morales, 2008). Tradescantia pallida plants have been widely used to perform micronuclei test provingto be an excellent biondicator for environmental quality assessment (Garcia et al, 2011;Leal, Crispim, Frota, Kelecom, & Silva, 2008;Thewes, Endres Júnior, & Droste, 2011).…”

“…Tradescantia pallida plants have been widely used to perform micronuclei test provingto be an excellent biondicator for environmental quality assessment (Garcia et al, 2011;Leal, Crispim, Frota, Kelecom, & Silva, 2008;Thewes, Endres Júnior, & Droste, 2011). In the same way, the use of Oreochromis niloticus fish due to its capability of adaptation to different environmental conditions, have also been extensively used in freshwater reservoirs to perform this mutagenic assay (Hoshina et al, 2008;Carvalho, Bernusso, Araújo, Espíndola, & Fernandes, 2012;Soliman & Ibrahim, 2012). Facing a probably natural contamination due to the geological characteristics of the Brazilian semiarid region, the adverse climatic conditions that lead to restrict the water availability for the population and the absence of studies of water quality of the Riacho das Cachoeiras Dam, a strategic regional water reservoir, the present study aimed to evaluate water quality for human consumption considering i) the assessment of radon and metal contamination and ii) the evaluation of mutagenic effect using micronuclei tests in Tradescantia pallida and Oreochromis niloticus..…”

Avaliação do potencial genotóxico e mutagênico de amostras de águas de recursos hídricos que recebem efluentes urbanos e industriais do pólo ceramista da cidade de Santa Gertrudes - SP

“…Em geral, para a realização do ensaio do micronúcleo, podem ser amostradas células de vários tecidos como brânquias e epitélio [38], fígado [39], rim [10,40,41] e sangue periférico [40][41][42][43][44][45][46][47][48]. No entanto, Hayashi et al [30] [42,45], o cádmio [10], o cromo [43,46], o mercúrio e o selênio [43], a mitomicina C [40,44], a ciclofosfamida [10] e o raio-X [44].…”