Induction of microsomal cytochrome P450s by tire‐leachate compounds, habitat components of Aedes albopictus mosquito larvae

Abstract

Benzothiazole (BZT) and its derivatives are the major leachate compounds of automobile tires, the principal breeding habitat of Aedes albopictus, particularly in the United States. Effects of the compounds on insecticide toxicity, and activities and expression of microsomal cytochrome P450s (P450s) in the mosquito larvae were examined. Mosquito larvae were more tolerant to carbaryl, rotenone, and temephos when they were pre-exposed to tire-leachate compounds, particularly BZT. There was no change in toxicity from the aldrin treatment by BZT. The effect of BZT was reversed when a P450 inhibitor, piperonyl butoxide, was applied in admixture with the insecticides. Microsomes from BZT-treated larvae had increased peroxidation activity of tetramethylbenzidine. This correlated with increased intensity of SDS-PAGE protein bands corresponding to molecular weights of 59 and 62 kD, which were detected as heme-containing proteins, a characteristic of P450s. The results suggest that BZT induces P450s, which detoxify insecticides and thus cause insecticide tolerance in the mosquito larvae. Arch.

Biochemical characterization of deltamethrin resistance in a laboratory-selected strain of Aedes aegypti

“…Our study also revealed monomorphic CYTP450 that was more intense in DR strain. A similar correlation was encountered in Aedes albopictus (Suwanchaichinda and Brattsten 2002).…”

Actual contamination of the Danube and Sava rivers at Belgrade (2013)

“…27 TBP was found in small amounts in all samples, except in the sample from near Vinča where higher concentrations were detected. 30 It could be associated with the residues of automobile tires. They are well known contaminants in river water, sewage effluents and river sediments.…”

The role of environment in shaping the genetic diversity of the subalpine mosquito, Aedes rusticus (Diptera, Culicidae)

“…Although the phenomenon of Bt resistance has been associated with a mutation of gut receptors, this insecticide may have a role in the selection of larvae with better detoxifying abilities. Such a cumulative impact of both environmental and man‐made chemical variables on the evolution of metabolic resistance against xenobiotics is widely observed in mosquito populations (Suwanchaichinda & Brattsten 2002).…”

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