Induction of mitochondrial aldehyde dehydrogenase by submergence facilitates oxidation of acetaldehyde during re‐aeration in rice

Tsuji, Hiroyuki; Meguro, Naoki; Suzuki, Yasuhiro; Tsutsumi, Nobuhiro; Hirai, Atsushi; Nakazono, Mikio

doi:10.1016/s0014-5793(03)00631-8

Cited by 81 publications

(91 citation statements)

References 38 publications

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“…Mitochondrial isoforms of AlDh2 are encoded by at least two gene copies in the rice nuclear genome. ALDH2a gene was expressed in various organs at much higher levels than ALDH2b in darkgrown seedlings under submergence (13,17,18). our results showed that ALDH2a gene expression was comparable in both dark-grown and light/dark-grown rice seedlings under submergence (Fig.…”

Section: Resultsmentioning

confidence: 50%

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Submergence-InducedADHandALDHGene Expression inJaponicaandIndicaRice with Contrasting Levels of Seedling Vigor under Submergence Stress

Manangkil

Mori

et al. 2009

Biotechnology & Biotechnological Equipment

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Section: Resultsmentioning

confidence: 50%

“…2). tsuji et al (18) suggested that rice ALDH2a mRnA was accumulated during submergence in order to quickly metabolize acetaldehyde produced upon reaeration. expression of ALDH2a gene was markedly enhanced under submergence, while that of ALDH2b gene was almost completely inhibited (Fig.…”

Section: Resultsmentioning

confidence: 99%

Submergence-InducedADHandALDHGene Expression inJaponicaandIndicaRice with Contrasting Levels of Seedling Vigor under Submergence Stress

Manangkil

Mori

et al. 2009

Biotechnology & Biotechnological Equipment

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“…2). Expression of ALDH2a is usually associated with the ability to metabolize acetaldehyde during reaeration because, although mRNA accumulates during low-oxygen treatment and not upon reaeration, enzyme activity increases only when plants are transferred from submergence to aerobic conditions (Tsuji et al, 2003). ALDH activity correlates with anoxia tolerance in varieties of Echinocloa crus-galli and it has been proposed that ALDH may detoxify acetaldehyde formed through alcoholic fermentation during anaerobic germination (Fukao et al, 2003).…”

Section: Anaerobic Responses In Ricementioning

confidence: 99%

Transcript Profiling of the Anoxic Rice Coleoptile

Lasanthi-Kudahettige

Magneschi²,

Loreti³

et al. 2007

Plant Physiology

301

369

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Rice (Oryza sativa) seeds can germinate in the complete absence of oxygen. Under anoxia, the rice coleoptile elongates, reaching a length greater than that of the aerobic one. In this article, we compared and investigated the transcriptome of rice coleoptiles grown under aerobic and anaerobic conditions. The results allow drawing a detailed picture of the modulation of the transcripts involved in anaerobic carbohydrate metabolism, suggesting up-regulation of the steps required to produce and metabolize pyruvate and its derivatives. Sugars appear to play a signaling role under anoxia, with several genes indirectly up-regulated by anoxia-driven sugar starvation. Analysis of the effects of anoxia on the expansin gene families revealed that EXPA7 and EXPB12 are likely to be involved in rice coleoptile elongation under anoxia. Genes coding for ethylene response factors and heat shock proteins are among the genes modulated by anoxia in both rice and Arabidopsis (Arabidopsis thaliana). Identification of anoxiainduced ethylene response factors is suggestive because genes belonging to this gene family play a crucial role in rice tolerance to submergence, a process closely related to, but independent from, the ability to germinate under anoxia. Genes coding for some enzymes requiring oxygen for their activity are dramatically down-regulated under anoxia, suggesting the existence of an energysaving strategy in the regulation of gene expression.Higher plants are aerobic organisms that rapidly die when oxygen availability is limited due to soil flooding (Voesenek et al., 2006). Species originating from semiaquatic environments are, however, able to cope with flooding stress. They can survive complete submergence for weeks and some even have the capacity to grow vigorously and produce flowers and seeds in permanently water-saturated soils. In this context, a wellknown crop is rice (Oryza sativa), which produces high yields even when it is grown in water-logged rice paddies. A broad range of metabolic and morphological adaptations characterizes these tolerant species. Floodtolerant plants have developed the capacity to generate ATP without the presence of oxygen (fermentative metabolism) and/or to develop specific morphologies (e.g. air channels, enhanced shoot elongation) that improve the entrance of oxygen

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“…Transcript abundance from nuclearencoded genes for components of both the cytochrome and alternative oxidases in mitochondria were reduced during anoxia and induced by air adaptation. The same authors have shown recently a specific isoform of mitochondrial aldehyde dehydrogenase (ALDH2a) is induced on re-aeration of hypoxic rice seedlings, putatively to defend against damage from acetaldehyde accumulation post-submergence (24).…”

mentioning

confidence: 99%

Changes in the Mitochondrial Proteome during the Anoxia to Air Transition in Rice Focus around Cytochrome-containing Respiratory Complexes

Millar

Trend

Heazlewood

2004

Journal of Biological Chemistry

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The ability of rice seedlings to grow from dry seed under anoxia provides a rare opportunity in a multicellular eukaryote to study the stages of mitochondrial biogenesis triggered by oxygen availability. The function and proteome of rice mitochondria synthesized under 6 days of anoxia following 1 day of air adaptation have been compared with mitochondria isolated from 7-day aerobically grown rice seedlings. Rice coleoptiles grown under anoxia, and the mitochondria isolated from them respired very slowly compared with airadapted and air-grown seedlings. Immunodetection of key mitochondrial protein markers, isoelectric focusing electrophoresis followed by SDS-PAGE to make soluble mitochondria proteome maps, and shotgun sequencing of mitochondrial proteins by liquid chromatographytandem mass spectrometry all revealed similar patterns of the major function categories of mitochondrial proteins from both anoxic and air-adapted samples. Activity analysis showed respiratory oxidases markedly increased in activity during the air adaptation of seedlings. Blue-native electrophoresis followed by SDS-PAGE of mitochondrial membrane proteins clearly showed the very low abundance of assembled b/c 1 complex and cytochrome c oxidase complex in the mitochondrial membrane in anoxic samples and the dramatic increase in the abundance of these complexes on air adaptation. Total heme content, cytochrome absorbance spectra, and the electron carrier, cytochrome c, also increased markedly on air adaptation. These results likely reflect limited heme synthesis for cytochrome assembly in the absence of oxygen and represent a discrete and reversible blockage of full mitochondrial biogenesis in this anoxia-tolerant species.

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Induction of mitochondrial aldehyde dehydrogenase by submergence facilitates oxidation of acetaldehyde during re‐aeration in rice

Cited by 81 publications

References 38 publications

Submergence-InducedADHandALDHGene Expression inJaponicaandIndicaRice with Contrasting Levels of Seedling Vigor under Submergence Stress

Submergence-InducedADHandALDHGene Expression inJaponicaandIndicaRice with Contrasting Levels of Seedling Vigor under Submergence Stress

Transcript Profiling of the Anoxic Rice Coleoptile

Changes in the Mitochondrial Proteome during the Anoxia to Air Transition in Rice Focus around Cytochrome-containing Respiratory Complexes

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