Induction of Mitotic Catastrophe in Human Cervical Cancer Cells After Administration of Aloe-emodin

Trybus, Wojciech; Król, Teodora; Trybus, Ewa; Stachurska, Anna; Kopacz-Bednarska, Anna; Król, Grzegorz

doi:10.21873/anticanres.12443

Cited by 13 publications

(7 citation statements)

References 19 publications

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“…Emodin also induced the degradation of phalloidin-labeled actin filaments, as well as the formation of its aggregates in the cytoplasm of cells. Similar changes have also been observed in the tested cells following the action of another anthraquinone, aloe-emodin [ 53 ]. The effect of the combined action of emodin and vinblastine was the intensification of changes in the structure of the cytoskeleton, as documented in Figure 9 , where numerous actin deposits can be observed, and its condensation is also visible in the rounded cells.…”

Section: Discussionsupporting

confidence: 79%

Emodin Sensitizes Cervical Cancer Cells to Vinblastine by Inducing Apoptosis and Mitotic Death

Trybus

Trybus²,

Król³

2022

IJMS

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In recent years, studies on the effects of combining novel plant compounds with cytostatics used in cancer therapy have received considerable attention. Since emodin sensitizes tumor cells to chemotherapeutics, we evaluated changes in cervical cancer cells after its combination with the antimitotic drug vinblastine. Cellular changes were demonstrated using optical, fluorescence, confocal and electron microscopy. Cell viability was assessed by MTT assay. The level of apoptosis, caspase 3/7, Bcl-2 protein, ROS, mitochondrial membrane depolarization, cell cycle and degree of DNA damage were analyzed by flow cytometry. The microscopic image showed indicators characteristic for emodin- and vinblastine-induced mitotic catastrophe, i.e., multinucleated cells, giant cells, cells with micronuclei, and abnormal mitotic figures. These compounds also increased blocking of cells in the G2/M phase, and the generated ROS induced swelling and mitochondrial damage. This translated into the growth of apoptotic cells with active caspase 3/7 and inactivation of Bcl-2 protein and active ATM kinase. Emodin potentiated the cytotoxic effect of vinblastine, increasing oxidative stress, mitotic catastrophe and apoptosis. Preliminary studies show that the combined action of both compounds, may constitute an interesting form of anticancer therapy.

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Section: Discussionsupporting

confidence: 79%

Emodin Sensitizes Cervical Cancer Cells to Vinblastine by Inducing Apoptosis and Mitotic Death

Trybus

Trybus²,

Król³

2022

IJMS

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“…Trybus W et al, 2018 [18] Induction of Mitotic Catastrophe in Human Cervical Cancer Cells After Administration of Aloe-emodin.…”

Section: Autor/ano Título Metodologiamentioning

confidence: 99%

Evidências sobre a ação de compostos do Aloe vera em células cancerígenas: uma revisão da literatura

Borges¹,

Hoefel

2022

Rev Fitos

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A pesquisa tem como objetivo encontrar evidências científicas que apontem a ação anticancerígena dos compostos aloe-emodina e aloína em células neoplásicas. Este estudo consiste em uma revisão bibliográfica sobre o tema, de artigos publicados em revistas indexadas nas bases de dados online PubMed, Lilacs e SciELO, utilizando os descritores de forma combinada: “câncer”, “aloe-emodina” e “aloína”. As buscas foram realizadas em março de 2020, optando por artigos publicados nos últimos 5 anos, nos idiomas inglês e português. Foram selecionados artigos que atendam aos seguintes critérios de inclusão: artigos originais; ensaios clínicos; estudos pré-clínicos e que se enquadram no tema pesquisado. Após aplicar os critérios de inclusão, foram selecionados 15 artigos que abordassem o efeito antineoplásico dos componentes do Aloe vera em células cancerígenas humanas. Os estudos in vitro e in vivo citados neste trabalho revelaram que os compostos derivados do Aloe vera (aloe-emodina e aloína) tiveram resultados satisfatórios quanto à atividade antineoplásica em diferentes linhas celulares de câncer humano. Porém, essas substâncias não foram testadas diretamente em seres humanos, revelando que são necessários experimentos clínicos para comprovar a eficácia do Aloe vera no tratamento do câncer.

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“…Aloe-emodin inhibits cell cycle progression, proliferation, growth, and survival, but enhances the apoptosis and radiosensitivity of CC cells. 154 -156 The anticancer mechanisms of caffeic acid include the enhancement of apoptotic cell death, chemosensitivity, and cell cycle arrest and the blockage of the proliferation and EMT of CC cells; these pharmacological effects are exhibited via the modulation of transforming growth factor (TGF)-β, HIF-1, p53, mitochondrial, and adenosine monophosphate-activated protein kinase (AMPK) pathways. 157 -161 Catechins possess anticancer potential that may cause growth-suppression and apoptosis of CC cells mediated by caspase activation and induction of oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

A Comprehensive Understanding of the Anticancer Mechanisms of FDY2004 Against Cervical Cancer Based on Network Pharmacology

Lee¹,

Kang²,

Park³

et al. 2021

Natural Product Communications

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Herbal drugs are continuously being developed and used as effective therapeutics for various cancers, such as cervical cancer (CC); however, their mechanisms of action at a systemic level have not been explored fully. To study such mechanisms, we conducted a network pharmacological investigation of the anti-CC mechanisms of FDY2004, an herbal drug consisting of Moutan Radicis Cortex, Persicae Semen , and Rhei Radix et Rhizoma. We found that FDY2004 inhibited the viability of human CC cells. By performing pharmacokinetic evaluation and network analysis of the phytochemical components of FDY2004, we identified 29 bioactive components and their 116 CC-associated pharmacological targets. Gene ontology enrichment analysis showed that the modulation of cellular functions, such as apoptosis, growth, proliferation, and survival, might be mediated through the FDY2004 targets. The therapeutic targets were also key components of CC-associated oncogenic and tumor-suppressive pathways, including PI3K-Akt, human papillomavirus infection, IL-17, MAPK, TNF, focal adhesion, and viral carcinogenesis pathways. In conclusion, our data present a comprehensive insight for the mechanisms of the anti-CC properties of FDY2004.

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Induction of Mitotic Catastrophe in Human Cervical Cancer Cells After Administration of Aloe-emodin

Abstract: Aloe-emodin induces mitotic catastrophe in cervical cancer cells.

Cited by 13 publications

References 19 publications

Emodin Sensitizes Cervical Cancer Cells to Vinblastine by Inducing Apoptosis and Mitotic Death

Emodin Sensitizes Cervical Cancer Cells to Vinblastine by Inducing Apoptosis and Mitotic Death

Evidências sobre a ação de compostos do Aloe vera em células cancerígenas: uma revisão da literatura

A Comprehensive Understanding of the Anticancer Mechanisms of FDY2004 Against Cervical Cancer Based on Network Pharmacology

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