Induction of Mouse Peritoneum Mesenchymal Stem Cells into Germ Cell-Like Cells Using Follicular Fluid and Cumulus Cells-Conditioned Media

Mirzaeian, Leila; Eftekhari‐Yazdi, Poopak; Esfandiari, Fereshteh; Eivazkhani, Farideh; Valojerdi, Mojtaba Rezazadeh; Moini, Ashraf; Fathi, Rouhollah

doi:10.1089/scd.2018.0149

Cited by 15 publications

(12 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Besides, the repair and reconstruction of peritoneum mesothelial cells can be performed through the secretion of growth factors (bFGF and VEGF), cytokines, and extracellular matrix [142]. Characterized peritoneum MSCs (PeMSCs) were differentiated into ovarian celllike cells using 10% human follicular fluid and 50% human cumulus-CM for 21 days, and the expression of oocyte (Zp3 and Gdf9), germ cell (Ddx4 amd Dazl), GC (Amh), and theca cell (Lhr) markers was assessed [143]. Skin-derived MSCs (SMSCs) improved the ovarian follicle microenvironment of mice with POF, and the levels of pro-inflammatory cytokines TNF-α, TGF-β, IL-8, IL-6, IL-1β, and IFN-γ significantly decreased [144].…”

Section: Effects Of Other Mscs On Pofmentioning

confidence: 99%

Human mesenchymal stem cell treatment of premature ovarian failure: new challenges and opportunities

Shan

et al. 2021

Stem Cell Res Ther

View full text Add to dashboard Cite

Premature ovarian failure (POF) is one of the common disorders found in women leading to 1% female infertility. Clinical features of POF are hypoestrogenism or estrogen deficiency, increased gonadotropin level, and, most importantly, amenorrhea. With the development of regenerative medicine, human mesenchymal stem cell (hMSC) therapy brings new prospects for POF. This study aimed to describe the types of MSCs currently available for POF therapy, their biological characteristics, and their mechanism of action. It reviewed the latest findings on POF to provide the theoretical basis for further investigation and clinical therapy.

show abstract

Section: Effects Of Other Mscs On Pofmentioning

confidence: 99%

Human mesenchymal stem cell treatment of premature ovarian failure: new challenges and opportunities

Shan

et al. 2021

Stem Cell Res Ther

View full text Add to dashboard Cite

show abstract

“…Mirzaeian et al. (2018) achieved a same result when they induced mouse peritoneum mesenchymal stem cells with 10% human FF or 50% human CCM, resulting in round OLCs with 50 μm diameter at day 21 of differentiation [ 46 ]. On the basis of the results; the generated OLCS in FF group were larger than the CCM group.…”

Section: Discussionmentioning

confidence: 99%

Wharton's jelly derived mesenchymal stem cells differentiate into oocyte like cells in vitro by follicular fluid and cumulus cells conditioned medium

Zolfaghar

Mirzaeian

Beiki

et al. 2020

Heliyon

Self Cite

View full text Add to dashboard Cite

Wharton's jelly derived-mesenchymal stem cells (WJ-MSCs) have a same developmental origin with primordial germ cells. WJ-MSCs perhaps differentiate into oocyte and germ like-cells (OLCs/GLCs) in the presence of appropriate inducers. Human follicular fluid (FF) and cumulus cells conditioned medium (CCM) are naturally rich sources for oocyte development. The aim of this study was to evaluate WJ-MSCs potential for differentiating into OLCs and GLCs exposed to FF and CCM. WJ-MSCs were cultured in two different induction media (10% FF, 10% CCM) for 21 days. Morphological changes and expression of developmental genes were evaluated on days 0, 7, 14 and 21 of culture. Also, on 21 st day of culture, the expression of oocyte and germ cell proteins investigated using immunofluorescence staining. Appearance of round shaped cells from 7 th day onwards indicated that WJ-MSCs can differentiate into OLCs when exposed to FF and CCM. The size of produced OLCs and expression of oocyte specific genes and proteins were increased more positively in FF group rather than CCM group. Although, WJ-MSCs could differentiate into OLCs by FF and CCM, however, the induction potential of FF for producing OLCs was better than CCM.

show abstract

“…[41][42][43][44] One of the sources of peritoneal mesothelium is the MPM. 20,34 Although previous studies have investigated the influences of decelluarized F I G U R E 2 DNA content of intact and decellularized IMPM and DMPM (n = 4 for each group). IMPM and DMPM.…”

Section: Discussionmentioning

confidence: 99%

“…In an earlier study, the presence of mesothelial stem cells in MPM was confirmed. 34 Under sterile conditions, the MPM of the adult NMRI mice (6-8 weeks old) were isolated and washed in PBS containing penicillin and streptomycin. Finally, it was cultured in a DMEM medium containing glutamine and nonessential amino acids as explants for a week.…”

Section: Preparation Of Pmscs As a Feeder Layer And Collection Of Their Conditioned Mediummentioning

confidence: 99%

Co‐culture with peritoneum mesothelial stem cells supports the in vitro growth of mouse ovarian follicles

Sarabadani

Tavana

Mirzaeian

et al. 2021

J Biomedical Materials Res

Self Cite

View full text Add to dashboard Cite

The important roles played by the ovarian microenvironment and cell interactions in folliculogenesis suggest promising approaches for in vivo growth of ovarian follicles using appropriate scaffolds containing suitable cell sources. In this study, we have investigated the growth of early preantral follicles in the presence of decellularized mesenteric peritoneal membrane (MPM), peritoneum mesothelial stem cells (PMSCs), and conditioned medium (CM) of PMSCs. MPM of mouse was first decellularized; PMSCs were isolated from MPM and cultured and their conditioned medium (CM) was collected. Mouse follicles were separated into four groups: (1) culture in base medium (control), (2) culture in decellularized MPM (DMPM), (3) co-culture with PMSCs (Co-PMSCs), and (4) culture in CM of PMSCs (CM-PMSCs). Qualitative and quantitative assessments were performed to evaluate intact mesenteric peritoneal membrane (IMPM) as well as decellularized ones. After culturing the ovarian follicles, follicular and oocyte diameter, viability, eccentric oocyte percentage, and estradiol hormone amounts were evaluated. Quantitative and qualitative evaluations confirmed removal of cells and retention of the essential fibers in MPM after the decellularization process. Follicular parameters showed that Co-PMSCs better support in vitro growth and development of ovarian follicles than the other groups. The eccentric rate and estradiol production were statistically higher for the Co-PMSCs group than for the CM-PMSCs and control groups. Although the culture of early preantral follicles on DMPM and CM-PMSCs could improve in vitro follicular growth, co-culture of follicles with PMSCs showed even greater improvements in terms of follicular growth and diameter. K E Y W O R D S conditioned medium, decellularized ECM, mesenteric peritoneal membrane, mesothelial stem cells, ovarian follicle culture | INTRODUCTIONChemo-and radiation therapies lead to early menopause, premature ovarian insufficiency (POI), and/or infertility in women. 1,2 One solution to this problem is the transplantation of fresh or frozen ovaries and ovarian tissues, but the risks of recurrence of malignancy in the cells and allogenic rejection of transplants have led to interest in the development of alternate techniques such as in vitro culture of isolated ovarian follicles. [2][3][4][5] Current follicular culture systems are inefficient due to inconsistent development of both somatic and germinal components of the ovarian follicles. 6 Moreover, in vitro cultured follicles have much smaller diameters and are poorer in quality than in vivo follicles. 7 There have been many studies in recent years aimed at improving in vitro follicle development through the use of various growth factors, supplements, and serum components. 6,8 The use of different matrices and co-cultures with supportive somatic cells and/or their

show abstract

Induction of Mouse Peritoneum Mesenchymal Stem Cells into Germ Cell-Like Cells Using Follicular Fluid and Cumulus Cells-Conditioned Media

Cited by 15 publications

References 25 publications

Human mesenchymal stem cell treatment of premature ovarian failure: new challenges and opportunities

Human mesenchymal stem cell treatment of premature ovarian failure: new challenges and opportunities

Wharton's jelly derived mesenchymal stem cells differentiate into oocyte like cells in vitro by follicular fluid and cumulus cells conditioned medium

Co‐culture with peritoneum mesothelial stem cells supports the in vitro growth of mouse ovarian follicles

Contact Info

Product

Resources

About