Induction of mucosal and systemic humoral immune responses in murine system by intranasal immunization with peptide antigens of P. vivax and CpG oligodeoxynucleotide (ODN) in microparticle delivery

Bhat, Ajaz A.; Seth, Ratanesh; Babu, J. Moses; Biswas, Subrata; Rao, D. N.

doi:10.1016/j.intimp.2009.06.008

Cited by 17 publications

(10 citation statements)

References 63 publications

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“…Similarly, synthetic CpG-ODNs can stimulate B cells, monocytes, macrophages and dendritic cells (DCs), inducing the secretion of pro-inflammatory and type 1 cytokines, resulting in the generation of a type 1-biased immune response [31][32][33]. CpG-ODNs have shown to enhance antibody response in mice for P.Vivax-, HIV-, anthraxand Y. pestis-derived epitopic sequences in mice model [20,32,34]. In our study, splenocytes, LP and PP cells obtained from F1 MAP with CpG-ODN-immunized mice and stimulated with F1-MAP showed enhanced T cell proliferation in vitro in different strains of mice.…”

Section: Discussionmentioning

confidence: 99%

Multiple Antigen Peptide Containing B and T Cell Epitopes of F1 Antigen of Yersinia pestis Showed Enhanced Th1 Immune Response in Murine Model

et al. 2013

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Yersinia pestis is a facultative bacterium that can survive and proliferate inside host macrophages and cause bubonic, pneumonic and systemic infection. Apart from humoral response, cell-mediated protection plays a major role in combating the disease. + showing Th1 response. Thus, the study highlights the importance of Th1 cytokine and existence of CD4 + and CD8 + immune response. This study proposes a new perspective for the development of vaccination strategies for Y. pestis that trigger T cell immune response.

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Section: Discussionmentioning

confidence: 99%

Multiple Antigen Peptide Containing B and T Cell Epitopes of F1 Antigen of Yersinia pestis Showed Enhanced Th1 Immune Response in Murine Model

et al. 2013

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“…Synthetic oligodeoxynucleotides with a complete phosphorothioate backbone containing two optimal mouse CpG motifs (ODN1826, TCC ATG ACG TTC CTG ACG TT; Operon Biotechnologies GmbH, Germany) were used as adjuvant (7).…”

Section: Methodsmentioning

confidence: 99%

Glycoprotein G of Herpes Simplex Virus 2 as a Novel Vaccine Antigen for Immunity to Genital and Neurological Disease

Görander

Harandi

Lindqvist

et al. 2012

J Virol

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The envelope glycoproteins of herpes simplex virus 1 (HSV-1) and HSV-2, with the exception of glycoprotein G, elicit cross-reactive B-and T-cell responses. Human vaccine trials, using the cross-reactive glycoproteins B and D, have shown no protection against genital HSV-2 infection or disease. In this study, the mature form of glycoprotein G (mgG-2) of HSV-2 was used for immunization of mice, either alone or in combination with adjuvant CpG, followed by an intravaginal challenge with a lethal dose of a fully virulent HSV-2 strain. Mice immunized with mgG-2 plus CpG showed low disease scores and a significantly higher survival rate (73%) than mice immunized with mgG-2 alone (20%) or controls (0%). Accordingly, limited numbers of infectious HSV-2 particles were detected in the spinal cord of mice immunized with mgG-2 plus CpG. The observed protection was associated with a gamma interferon (IFN-␥) response by splenic CD4 ؉ T cells upon antigen restimulation in vitro and in vaginal washes 1 day postinfection. The majority of sera collected from mice immunized with mgG-2 plus CpG showed macrophagemediated antibody-dependent cellular cytotoxicity and antibody-dependent complement-mediated cytolysis, while no neutralization activity was observed. In conclusion, we have shown that immunization with the type-specific mgG-2 protein in combination with CpG could elicit protective immunity against an otherwise lethal vaginal HSV-2 challenge. The mgG-2 protein may therefore constitute a promising HSV-2 vaccine antigen to be considered for future human trials.

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“…Sera of mice contained predominantly IgG2a/IgG2b isotypes and anti-Pvs24 antiserum significantly reduced the oocyst development in mosquito midgut during mosquito membrane feeding assay. Immunofluorescence assay showed strong immunoreactivity of peptide specific antisera with pre-erythrocytic and erythrocytic antigens of the parasite (Bhat et al, 2009). This observation promoted us to study the cellmediated immune response in terms of lymphoproliferative response and cytokine levels in three compartments viz.…”

Section: Introductionmentioning

confidence: 94%

“…The peptide antigens investigated in the present study are immunodominant and conserved sequences in parasite (Bhat et al, 2009). Peptide-based vaccines focus the host's immune response on specific epitopes known to play a role in protective immunity.…”

Section: Introductionmentioning

confidence: 99%

Induction of Cell-Mediated Immune Responses to Peptide Antigens ofP. vivaxin Microparticles Using Intranasal Immunization

Bhat

Seth

Kumar

et al. 2010

Immunological Investigations

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T-cells play a critical role in resistance to malaria, not only because they function as helper cells for an antibody response, but also because they serve as effector cells. Such cellular immunity is directly implicated in protection from sporozoites as well as from blood stage parasites. The aim of this study was to induce cell mediated immune responses to peptide antigens of Plasmodium vivax co-encapsulated with CpG oligodeoxynucleotide (ODN) in microparticles. In the present study, we have investigated the immunomodulatory effects of two CpG adjuvants, CpG 1826 and CpG 2006 to the five peptide antigens of Plasmodium vivax derived from circumsporozoite protein, merozoite surface protein-1, apical membrane antigen-1 and gametocyte surface antigen (Pvs24) in microparticle delivery. The T-cell proliferation response study of the cells collected from spleen, lamina propria and peyer's patches showed significantly high (p<0.001) stimulation index when primed with peptide antigens in microparticles co-encapsulating CpG ODN adjuvant as compared to peptide alone primed mice. The cytokine measurement profile of IFN-gamma, TNF-alpha, IL-2, IL-4 and IL-10 in culture supernatants of cells primed with peptide antigens in microparticles co-encapsulating CpG ODN showed higher levels of IFN- gamma followed by TNF-alpha and IL-2, with relatively low levels of IL-4 and IL-10.

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Induction of mucosal and systemic humoral immune responses in murine system by intranasal immunization with peptide antigens of P. vivax and CpG oligodeoxynucleotide (ODN) in microparticle delivery

Cited by 17 publications

References 63 publications

Multiple Antigen Peptide Containing B and T Cell Epitopes of F1 Antigen of Yersinia pestis Showed Enhanced Th1 Immune Response in Murine Model

Multiple Antigen Peptide Containing B and T Cell Epitopes of F1 Antigen of Yersinia pestis Showed Enhanced Th1 Immune Response in Murine Model

Glycoprotein G of Herpes Simplex Virus 2 as a Novel Vaccine Antigen for Immunity to Genital and Neurological Disease

Induction of Cell-Mediated Immune Responses to Peptide Antigens ofP. vivaxin Microparticles Using Intranasal Immunization

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