Induction of multi-epitopespecific antibodies against HIV-1 by multi-epitopevaccines

Ding, Jun; He, Fei; Lu, Yun; Xiao, Yi; Chen, Yinghua

doi:10.1007/bf02901157

Cited by 7 publications

(5 citation statements)

References 21 publications

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“…The epitope vaccine has higher safety than the traditional live attenuated vaccine. A large number of epitope vaccines have been tried on other pathogens and have good vaccine efficacy [ 38 , 39 ]. In addition, the ability of epitope vaccines to address mutations in virus strains is incomparably better than that of traditional vaccines, especially for viruses whose wild strains have strong mutation abilities, such as CDV.…”

Section: Discussionmentioning

confidence: 99%

Self-assembling ferritin nanoparticles coupled with linear sequences from canine distemper virus haemagglutinin protein elicit robust immune responses

Wang

Qiao

et al. 2022

J Nanobiotechnol

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Background Canine distemper virus (CDV), which is highly infectious, has caused outbreaks of varying scales in domestic and wild animals worldwide, so the development of a high-efficiency vaccine has broad application prospects. Currently, the commercial vaccine of CDV is an attenuated vaccine, which has the disadvantages of a complex preparation process, high cost and safety risk. It is necessary to develop a safe and effective CDV vaccine that is easy to produce on a large scale. In this study, sequences of CDV haemagglutinin (HA) from the Yanaka strain were aligned, and three potential linear sequences, termed YaH3, YaH4, and YaH5, were collected. To increase the immunogenicity of the epitopes, ferritin was employed as a self-assembling nanoparticle element. The ferritin-coupled forms were termed YaH3F, YaH4F, and YaH5F, respectively. A full-length HA sequence coupled with ferritin was also constructed as a DNA vaccine to compare the immunogenicity of nanoparticles in prokaryotic expression. Result The self-assembly morphology of the proteins from prokaryotic expression was verified by transmission electron microscopy. All the proteins self-assembled into nanoparticles. The expression of the DNA vaccine YaHF in HEK-293T cells was also confirmed in vitro. After subcutaneous injection of epitope nanoparticles or intramuscular injection of DNA YaHF, all vaccines induced strong serum titres, and long-term potency of antibodies in serum could be detected after 84 days. Strong anti-CDV neutralizing activities were observed in both the YaH4F group and YaHF group. According to antibody typing and cytokine detection, YaH4F can induce both Th1 and Th2 immune responses. The results of flow cytometry detection indicated that compared with the control group, all the immunogens elicited an increase in CD3. Simultaneously, the serum antibodies induced by YaH4F and YaHF could significantly enhance the ADCC effect compared with the control group, indicating that the antibodies in the serum effectively recognized the antigens on the cell surface and induced NK cells to kill infected cells directly. Conclusions YaH4F self-assembling nanoparticle obtained by prokaryotic expression has no less of an immune effect than YaHF, and H4 has great potential to become a key target for the easy and rapid preparation of epitope vaccines. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

Self-assembling ferritin nanoparticles coupled with linear sequences from canine distemper virus haemagglutinin protein elicit robust immune responses

Wang

Qiao

et al. 2022

J Nanobiotechnol

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“…For the primitive cell, the angle constants α, β, and c are 90 °. e axial length constants a, b, and c are 3.776 Å, 3.776 Å, and 9.486 Å, respectively [2][3][4]. Figure 1 shows the schematical picture of crystal geometry and basis vector direction for anatase TiO 2 in which the red sphere represents oxygen and the gray sphere represents titanium.…”

Section: Models and Computational Detailsmentioning

confidence: 99%

“…Titanium dioxide (TiO 2 ) is a kind of environmentally friendly material, and it has advantages such as chemical stability, easy fabrication, nontoxic release, and cheap raw material. TiO 2 matrix materials can be applied widely in fields such as coatings, rubbers, fibers, cosmetics, functional building materials, and modern electronic industries [1][2][3][4][5]. In general, the TiO 2 matrix materials can be prepared experimentally by the hydrothermal method, sol-gel method, and solid-state reaction method, and the method described by Anh et al [3].…”

Section: Introductionmentioning

confidence: 99%

Oxygen Dependence of Formation, Electronic State Transition, and Spin Polarization for Anatase TiO2: A Comprehensive Study

Shan

Fan

Sun

et al. 2022

Advances in Condensed Matter Physics

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The stability, geometry, microstructure, and specie combination together with the electronic states of the anatase TiO2 with oxygen defect content of 0%, 3.125%, 6.25%, and 12.5% have been intensively studied within the framework of the density functional theory method. The results show that the TiO2 with an oxygen defect is not as stable as intrinsic TiO2. The compound formation enthalpy Ef and the oxygen defect formation energy value tend to be larger for a higher defect content, and the oxygen defect gets harder to be formed. The bonds within the TiO6 polyhedron are different and not geometrically symmetrical. The bond strengths show distinct diversity, and the primitive cell of anatase TiO2 show spatial expansion when there are oxygen defects. All bands moved down to the low energy region, and two impurity energy band levels emerged for the anatase TiO2 with oxygen defect. The energy band gap is decreased from 3.085 eV to 1.165 eV, 1.0015 eV, and 0.43 eV. There are generally 7 peaks for the spin density of states function, corresponding to their 5 main bands. For the anatase TiO2 with an oxygen defect content of 12.5%, the spin density of states functions are not horizontal ordinate symmetrical near −1.12 eV and 0.31 eV. They are formed by oxygen defect energy levels, which is the result of the Ti d and O p state electron polarization. Transitions from weak paramagnetic to antiferromagnetic are found for the anatase TiO2 with oxygen defect.

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“…Some of the genes related to pollen development have been cloned. For example, tapetum-specific genes RA8 and RA39 (Jeon et al 1999;Ding et al 2002); tapetum-and microspore-specific gene OsPRP1 (Wu et al 2003); floral-preferentially expressed gene RA68 (Wu and Wang 2004); anther-specific gene Profilin (Ye et al 2001); anther loculus-specific gene OsCP1 (Lee et al 2004); autochromosome synapsis-related gene in meiosis OsDMC1 (Ding et al 2001); and the chalcone synthase-like gene D5 (Zheng et al 2000). Although there have been many studies dealing with pollen development, the underlying molecular mechanisms of rice pollen development remain largely unknown.…”

Section: Introductionmentioning

confidence: 98%

Isolation and Characterization of a Microsporocyte-Specific Gene, OsMSP, in Rice

Jiang

et al. 2009

Plant Mol Biol Rep

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A spikelet-specific cDNA clone, OsMSP (Oryza sativa meiosis serine protease), was isolated from a rice cDNA library constructed by suppression subtraction hybridization (SSH). Its expression level was significantly reduced in the thermo-sensitive genic male-sterile rice line at sterile temperature (30/26°C, day/night) compared with that at fertile temperature (23/21°C, day/night) conditions. In contrast, no significant differences were observed in the normal fertile line under the above two sets of temperature conditions. The cDNA is 2,867 bp in length with an open reading frame (ORF) encoding 837 amino acid residues. Northern blots revealed that OsMSP transcripts were specifically present in spikelets at meiosis, and reached the highest level at early microspore stage. mRNA in situ hybridization confirmed that OsMSP was a rice microsporocyte-specific gene and its expression was limited to early microspore developmental stage. BLAST and phylogenetic analyses revealed that OsMSP shared high similarities with subtilisin-like serine protease genes from other organisms at both nucleotide and amino acid levels.

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Induction of multi-epitopespecific antibodies against HIV-1 by multi-epitopevaccines

Cited by 7 publications

References 21 publications

Self-assembling ferritin nanoparticles coupled with linear sequences from canine distemper virus haemagglutinin protein elicit robust immune responses

Self-assembling ferritin nanoparticles coupled with linear sequences from canine distemper virus haemagglutinin protein elicit robust immune responses

Oxygen Dependence of Formation, Electronic State Transition, and Spin Polarization for Anatase TiO2: A Comprehensive Study

Isolation and Characterization of a Microsporocyte-Specific Gene, OsMSP, in Rice

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