Induction of Mutation in Streptococcus diacetilactis by Nitrosoguanidine and Ultraviolet Irradiation

Kuila, R. K.; Ranganathan, B.; Dutta, Santanu; Laxminarayana, H.

doi:10.3168/jds.s0022-0302(71)85838-1

Cited by 11 publications

(2 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Lactic acid bacteria starter strains, which play important roles in the processing of fermented dairy products, have been genetically improved to advance or stabilize desirable traits since the 1970s. In the early years, LAB strains were exposed to the mutagenic action of N-methyl- N′-nitro-N-nitrosoguanidine (MNNG) or to UV irradiation and desirable mutants were obtained (Kuila et al, 1971;Miyamoto et al, 1983). In the 1980s, LAB were found to have plasmid DNA sequences that code such significant traits as lactose metabolism, protease production, and citrate metabolism.…”

Section: Introductionmentioning

confidence: 99%

Binding of Mutagens to Exopolysaccharide Produced by Lactobacillus plantarum Mutant Strain 301102S

Tsuda

Hara

Miyamoto

2008

Journal of Dairy Science

View full text Add to dashboard Cite

Exopolysaccharide (EPS) was produced by Lactobacillus plantarum 301102 on exposure to the mutagenic action of acridine orange and novobiocin. The biological characteristics of this mutant strain 301102S were the same as those of the parent strain, but fermented milk prepared with the mutant strain showed antimutagenic activity on 3-amino-1,4-dimethyl-5H-pyrido indole. Only EPS-bound cells of strain 301102S showed binding ability to mutagens such as heterocyclic amines, and the mutagens were inactivated by binding to EPS. The binding ability was affected by pH; the greatest percentage binding was noted at pH 8.0. Addition of Mg(2+) and sodium dodecyl sulfate, but not oxgall, inhibited the binding ability. Therefore, the binding mechanism of the EPS may consist of ion-exchange and hydrophobic bonds, and the EPS would bind mutagens in the intestine.

show abstract

Section: Introductionmentioning

confidence: 99%

Binding of Mutagens to Exopolysaccharide Produced by Lactobacillus plantarum Mutant Strain 301102S

Tsuda

Hara

Miyamoto

2008

Journal of Dairy Science

View full text Add to dashboard Cite

show abstract

“…Chemical mutagenesis of LAB has been undertaken in a number of instances using N‐methyl‐N′‐nitro‐N‐nitrosoguanidine (NTG). NTG has been used to induce an increase in the formation of diacetyl and acetaldehyde in Streptococcus diacetylactis (Kuila et al. 1971).…”

Section: Introductionmentioning

confidence: 99%