Induction of neural differentiation in rat C6 glioma cells with taxol

Chao, Chuan-Chuan; Kan, Daphne; Lo, Ta-Hsuan; Lu, Kuo‐Shyan; Chien, Chung‐Liang

doi:10.1002/brb3.414

Cited by 23 publications

(12 citation statements)

References 37 publications

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“…It can be seen that GFAP was abundantly expressed in the normal brain tissue zone but was barely expressed in the glioma zone. This was consistent with the previous findings, which demonstrated that C6 cells remain in an undifferentiated state and cannot be recognized by GFAP [43]. Based on their histological traits and biological behaviour, C6 glioma cells have been reported to correspond to human anaplastic astrocytoma of grade II/III [44], indicating that QDs-c(RGDyk)NP might be used as a tracer of human glioma in the future.…”

Section: He and Immunohistochemistry Staining Of Gliomasupporting

confidence: 92%

c(RGDyk)-modified nanoparticles encapsulating quantum dots as a stable fluorescence probe for imaging-guided surgical resection of glioma under the auxiliary UTMD

Xiong

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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Surgical resection remains the preferred approach for some patients with glioblastoma (GBM), and eradication of the residual tumour niche after surgical resection is very helpful for prolonging patient survival. However, complete surgical resection of invasive GBM is difficult because of its ambiguous boundary. Herein, a novel targeting material, c(RGDyk)-poloxamer-188, was synthesized by modifying carboxyl-terminated poloxamer-188 with a glioma-targeting cyclopeptide, c(RGDyk). Quantum dots (QDs) as fluorescent probe were encapsulated into the self-assembled c(RGDyk)-poloxamer-188 polymer nanoparticles (NPs) to construct glioma-targeted QDs-c(RGDyk)NP for imaging-guided surgical resection of GBM. QDs-c(RGDyk)NP exhibited a moderate hydrodynamic diameter of 212.4 nm, a negative zeta potential of-10.1 mV and good stability. QDs-c(RGDyk)NP exhibited significantly lower toxicity against PC12 and C6 cells and HUVECs than free QDs. Moreover, in vitro cellular uptake experiments demonstrated that QDs-c(RGDyk)NP specifically targeted C6 cells, making them display strong fluorescence. Combined with ultrasound-targeted microbubble destruction (UTMD), QDs-c(RGDyk)NP specifically accumulated in glioma tissue in orthotropic tumour rats after intravenous administration, evidenced by ex vivo NIR fluorescence imaging of bulk brain and glioma tissue sections. Furthermore, fluorescence imaging with QDs-c(RGDyk)NP guided accurate surgical resection of glioma. Finally, the safety of QDs-c(RGDyk)NP was verified using pathological HE staining. In conclusion, QDs-c(RGDyk)NP may be a potential imaging probe for imaging-guided surgery.

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Section: He and Immunohistochemistry Staining Of Gliomasupporting

confidence: 92%

c(RGDyk)-modified nanoparticles encapsulating quantum dots as a stable fluorescence probe for imaging-guided surgical resection of glioma under the auxiliary UTMD

Xiong

et al. 2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…Induced neural differentiation of paclitaxel-treated C6 glioma cells through cell morphologic changes with expression of neural markers was recently reported [33]. Extended morphology of αB-crystallin-overexpressing cells but not αB-crystallin knockdown cells was dramatically reduced by a microtubule stabilization agent, paclitaxel, indicating that stretched morphology is dependent on αB-crystallin-assisted microtubule dynamic instability (Fig 5).…”

Section: Resultssupporting

confidence: 51%

Small Heat Shock Protein αB-Crystallin Controls Shape and Adhesion of Glioma and Myoblast Cells in the Absence of Stress

2016

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Cell shape and adhesion and their proper controls are fundamental for all biological systems. Mesenchymal cells migrate at an average rate of 6 to 60 μm/hr, depending on the extracellular matrix environment and cell signaling. Myotubes, fully differentiated muscle cells, are specialized for power-generation and therefore lose motility. Cell spreading and stabilities of focal adhesion are regulated by the critical protein vinculin from immature myoblast to mature costamere of differentiated myotubes where myofibril Z-band linked to sarcolemma. The Z-band is constituted from microtubules, intermediate filaments, cell adhesion molecules and other adapter proteins that communicate with the outer environment. Mesenchymal cells, including myoblast cells, convert actomyosin contraction forces to tension through mechano-responsive adhesion assembly complexes as Z-band equivalents. There is growing evidence that microtubule dynamics are involved in the generation of contractile forces; however, the roles of microtubules in cell adhesion dynamics are not well determined. Here, we show for the first time that αB-crystallin, a molecular chaperon for tubulin/microtubules, is involved in cell shape determination. Moreover, knockdown of this molecule caused myoblasts and glioma cells to lose their ability for adhesion as they tended to behave like migratory cells. Surprisingly, αB-crystallin knockdown in both C6 glial cells and L6 myoblast permitted cells to migrate more rapidly (2.7 times faster for C6 and 1.3 times faster for L6 cells) than dermal fibroblast. On the other hand, overexpression of αB-crystallin in cells led to an immortal phenotype because of persistent adhesion. Position of matured focal adhesion as visualized by vinculin immuno-staining, stress fiber direction, length, and density were clearly αB-crystallin dependent. These results indicate that the small HSP αB-crystallin has important roles for cell adhesion, and thus microtubule dynamics are necessary for persistent adhesion.

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“…Hence it will be of interest to investigate if NA affects the activation of GSK-3β and/or Akt and, if so, how these effects are related to intracellular calcium levels. Another important question is whether NA could induce the differentiation of glioma stem cells, as reported recently for other anti-cancer drugs such as taxol64. Although we did not observe any apparent changes in the level of stemness marker Nestin or differentiation marker β-tubulin with immunostaining (Figs 1 and 6J,K), more quantitative methods such as real-time RT-PCR and Western blot are needed to determine if there are any relatively subtle effects of NA on glioma differentiation.…”

Section: Discussionmentioning

confidence: 94%

Nicotinic acid inhibits glioma invasion by facilitating Snail1 degradation

Shi

et al. 2017

Sci Rep

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Malignant glioma is a formidable disease that commonly leads to death, mainly due to the invasion of tumor cells into neighboring tissues. Therefore, inhibition of tumor cell invasion may provide an effective therapy for malignant glioma. Here we report that nicotinic acid (NA), an essential vitamin, inhibits glioma cell invasion in vitro and in vivo. Treatment of the U251 glioma cells with NA in vitro results in reduced invasion, which is accompanied by a loss of mesenchymal phenotype and an increase in cell-cell adhesion. At the molecular level, transcription of the adherens junction protein E-cadherin is upregulated, leading to accumulation of E-cadherin protein at the cell-cell boundary. This can be attributed to NA’s ability to facilitate the ubiquitination and degradation of Snail1, a transcription factor that represses E-cadherin expression. Similarly, NA transiently inhibits neural crest migration in Xenopus embryos in a Snail1-dependent manner, indicating that the mechanism of action for NA in cell migration is evolutionarily conserved. We further show that NA injection blocks the infiltration of tumor cells into the adjacent brain tissues and improves animal survival in a rat model of glioma. These results suggest that NA treatment may be developed into a potential therapy for malignant glioma.

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Induction of neural differentiation in rat C6 glioma cells with taxol

Cited by 23 publications

References 37 publications

c(RGDyk)-modified nanoparticles encapsulating quantum dots as a stable fluorescence probe for imaging-guided surgical resection of glioma under the auxiliary UTMD

c(RGDyk)-modified nanoparticles encapsulating quantum dots as a stable fluorescence probe for imaging-guided surgical resection of glioma under the auxiliary UTMD

Small Heat Shock Protein αB-Crystallin Controls Shape and Adhesion of Glioma and Myoblast Cells in the Absence of Stress

Nicotinic acid inhibits glioma invasion by facilitating Snail1 degradation

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