Induction of Nutritional Mutants of Glutamic Acid Bacteria and Their Amino Acid Accumulation

“…In amino acid metablolism, some antagonism between different amino acids (16,17,18) and feed-back mechanisms (19,20) have already been known. Therefore, effects of many amino acids other than threonine on this fermentation were also examined.…”

Section: Results (I) Growing Culture Experiments With Chemically Defimentioning

confidence: 99%

“…(d) In the recent advance in f ermentative production of amino acids, auxotrophic mutants of M. glutamicus have played significant roles, for example, biotin-less strains in glutamic acid fermentation (21), homoserine-less strains in lysine fermentation (20), arginine-less strains in ornithine fermentation (30) and so forth. In order to achieve good yields of products in those fermentations, appropriate amounts of required substances were always necessary.…”

Section: Discussionmentioning

confidence: 99%

“…(a) Strains used : Among many auxotrophs of Micrococcus glutamicus isolated by NAKAYAMA et al (11) and many other species, two strains, i. e., M. glutamicus 534-185 and 534-Co147 (threonine-less mutants) were found to show a considerable accumulation of L-homoserine in their culture broths (7).…”

Section: Experimental Methods (I) Growing Culture Experimentsmentioning

confidence: 99%

“…(c) Effect of L-threonine on the enzyme activity for homoserine and lysine biosynthesis NAKAYAMA et al (20) reported that both threonine and homoserine strongly inhibited the enzyme activity for lysine production in the lysine fermentation with a homoserine requirring mutant of M. glutamicus.…”

Section: (Ii) Experiments With Intact Cell Suspensionmentioning

confidence: 99%

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Negative Feed-Back Control Caused by Threonine and Methionine in L-Homoserine Fermentation

Samejima

Nara

Fujita

et al. 1961

J. Gen. Appl. Microbiol.

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Lysine production from hydrocarbon by micrococcus species

Chatterjee

1982

Acta Biotechnologica

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SunimaryA bacterium isolated from Assam (India) soil was found to accumulate L-lysine in the mineral salt-hydrocarbon medium and identified to be a strain of Micrococcus luteus. The strain is able to grow and accumulate I-lysine in a purely synthetic medium, but supplementation of the synthetic medium with casamino acid or yeast extract or both, improves the yield. The entire fermentation period can be divided into a growth phase and a production phase, which can be prolonged by adjustment of the pH to the neutral range. Among the different hydrocarbon and nitrogen sources tested straight run gas oil (4%) and ammonium sulphate (0.4y0), respectively, were found most suitable. Erythromycin at 1 g / m l level inhibited growth but stimulated lysine excretion. An inoculum level of 10% (v/v) of the medium was optimal for lysine production. The yield of lysine under optimal conditions waa found to be 3.25 g per litre of the medium. Lysine has been isolated in crystalline form from the fermented broth by ion exchange resin chromatography and found to be pure sample of L-isomer.

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Induction of Nutritional Mutants of Glutamic Acid Bacteria and Their Amino Acid Accumulation

Cited by 28 publications

References 3 publications

STUDIES ON LYSINE FERMENTATION III. Α, Ε-Diaminopimelic ACID ACCUMULATION AND DIAMINOPIMELIC ACID DECARBOXYLASE

STUDIES ON LYSINE FERMENTATION III. Α, Ε-Diaminopimelic ACID ACCUMULATION AND DIAMINOPIMELIC ACID DECARBOXYLASE

Negative Feed-Back Control Caused by Threonine and Methionine in L-Homoserine Fermentation

Lysine production from hydrocarbon by micrococcus species

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